Characterization of Tunisian pomegranate (Punica granatum L.) cultivars using amplified fragment length polymorphism analysis

The amplified fragment length polymorphism (AFLP) analysis of DNA was used to characterize 34 pomegranate cultivars. By using a combination of six primers, a total of 327 markers were scored with a mean of 57.5. The high percentage of polymorphic bands (ppb) of 94.7 and the resolving power (Rp) collective rate value of 129.14 were scored. Data proved that the tested primers were informative to discriminate among cultivars and to survey the genetic diversity in this fruit crop. It has been assumed that the local pomegranate germplasm is characterized by a typically continuous genetic diversity. The derived dendrogram proved that cultivars are clustered independently from their geographical origin and their denomination. In addition, AFLP permitted the generation of a nearly unlimited number of molecular markers that are reliable in differentiating the cultivars and/or the polyclonal varieties.

[1]  S. Dellaporta,et al.  Maize DNA minipreps , 1983 .

[2]  O. Panaud,et al.  Dynamic conservation of apricot Prunus armeniaca in saharian oases: use of AFLP markers to assess genetic diversity in traditional orchards , 2002, Euphytica.

[3]  P. Vos,et al.  AFLP: a new technique for DNA fingerprinting. , 1995, Nucleic acids research.

[4]  A. Depicker,et al.  AFLP markers reveal high polymorphic rates in ryegrasses (Lolium spp.) , 2000, Molecular Breeding.

[5]  G. Levin Aspects of pomegranate culture in Turkmenistan , 1995 .

[6]  M. Nei,et al.  Mathematical model for studying genetic variation in terms of restriction endonucleases. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[7]  M. Marrakchi,et al.  Diversity of pomegranate (Punica granatum L.) germplasm in Tunisia , 1999, Genetic Resources and Crop Evolution.

[8]  O. Panaud,et al.  Exploration of intra- and inter-population genetic diversity in Hedysarum coronarium L. by AFLP markers , 2005, Genetic Resources and Crop Evolution.

[9]  M. T. Jackson,et al.  Are mapped markers more useful for assessing genetic diversity? , 2000, Theoretical and Applied Genetics.

[10]  S. Dellaporta Maize DNA miniprep , 1985 .

[11]  P. J. Maughan,et al.  Amplified fragment length polymorphism (AFLP) in soybean: species diversity, inheritance, and near-isogenic line analysis , 1996, Theoretical and Applied Genetics.

[12]  V. Laucou,et al.  Silver staining and recovery of AFLP amplification products on large denaturing polyacrylamide gels. , 1997, BioTechniques.

[13]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[14]  T. Moriguchi,et al.  In vitro adventitious shoot formation from anthers of pomegranate , 1987 .

[15]  U. Aksoy,et al.  Suitability of isozyme, RAPD and AFLP markers to assess genetic differences and relatedness among fig (Ficus carica L.) clones , 2001 .

[16]  R. Malmberg Molecular biology of plants , 1985 .

[17]  Roderic D. M. Page,et al.  TreeView: an application to display phylogenetic trees on personal computers , 1996, Comput. Appl. Biosci..

[18]  B J Bassam,et al.  Fast and sensitive silver staining of DNA in polyacrylamide gels. , 1991, Analytical biochemistry.

[19]  M. Wilkinson,et al.  A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars , 1999, Theoretical and Applied Genetics.

[20]  R. Michelmore,et al.  PCR-based fingerprinting using AFLPs as a tool for studying genetic relationships in Lactuca spp. , 1996, Theoretical and Applied Genetics.

[21]  K. Shimamoto,et al.  The Molecular Biology of Rice , 1995, Science.

[22]  A. Ebadi,et al.  RAPD markers reveal polymorphism among some Iranian pomegranate (Punica granatum L.) genotypes , 2006 .

[23]  P. Stam,et al.  Association between relationship measures based on AFLP markers, pedigree data and morphological traits in barley , 1997, Theoretical and Applied Genetics.

[24]  L. Guarino,et al.  Socotra: the Island of Bliss revisited. , 1990 .

[25]  R. Lewis,et al.  Developing an appropriate strategy to assess genetic variability in plant germplasm collections , 1999, Theoretical and Applied Genetics.

[26]  M. Metche,et al.  Quantitative determination of the polyphenolic content of pomegranate peel , 1996, Zeitschrift fur Lebensmittel-Untersuchung und -Forschung.

[27]  M. Knox,et al.  AFLP analysis of the diversity and phylogeny of Lens and its comparison with RAPD analysis , 1996, Theoretical and Applied Genetics.

[28]  R. Krueger,et al.  Genetic Analysis of Egyptian Date (Phoenix dactylifera L.) Accessions Using AFLP Markers , 2005, Genetic Resources and Crop Evolution.