Effects of adenosine on contractile response of circular muscle in electrically stimulated guinea-pig ileum.

The action of adenosine on the electrically induced mechanical response of circular muscle in isolated guinea-pig ileum has been investigated. Electrical stimulation (0.1 Hz) elicited the twitch response, which was completely abolished by tetrodotoxin (0.2 microM), morphine (1 microM) and atropine (0.1 microM). Adenosine (0.1-100 microM) markedly depressed the twitch response in a concentration-dependent manner, and the concentration-depression curve for adenosine was significantly shifted to the right in the presence of theophylline (30 microM). On the other hand, the contractile responses induced by acetylcholine (1-300 microM) were not effected by adenosine at all. The present investigation suggests that the twitch response is mediated through acetylcholine released from the intramural cholinergic nerves supplying the circular muscle of guinea-pig ileum, and adenosine has an inhibitory effect on the cholinergic transmission, probably via P1-purinoceptors.

[1]  E. Hayashi,et al.  Adenosine and dipyridamole: actions and interactions on the contractile response of guinea‐pig ileum to high frequency electrical field stimulation , 1985, British journal of pharmacology.

[2]  J. Cooper,et al.  Modulation of the release of acetylcholine from ileal synaptosomes by adenosine and adenosine 5'-triphosphate. , 1982, The Journal of pharmacology and experimental therapeutics.

[3]  E. Hayashi,et al.  Sites of actions of adenosine in intrinsic cholinergic nerves of ileal longitudinal muscle from guinea pig. , 1982, European journal of pharmacology.

[4]  J. Cooper,et al.  A Synaptosomal Preparation from the Guinea Pig Ileum Myenteric Plexus , 1981, Journal of neurochemistry.

[5]  M. Costa,et al.  Types of nerves in the enteric nervous system , 1980, Neuroscience.

[6]  T. Ozaki.  Effects of stimulation of Auerbach's plexus on both longitudinal and circular muscles. , 1979, The Japanese journal of physiology.

[7]  E. Hayashi,et al.  THE DEVELOPMENT OF TACHYPHYLAXIS TO ELECTRICAL STIMULATION IN GUINEA‐PIG ILEAL LONGITUDINAL MUSCLES AND THE POSSIBLE PARTICIPATION OF ADENOSINE AND ADENINE NUCLEOTIDES , 1978, British journal of pharmacology.

[8]  E. Hayashi,et al.  Effects of purine compounds on cholinergic nerves. Specificity of adenosine and related compounds on acetylcholine release in electircally stimulated guinea pig ileum. , 1978, European journal of pharmacology.

[9]  G. Burnstock.  A basis for distinguishing two types of purinergic receptor , 1978 .

[10]  K. Kitamura.  Comparative aspects of membrane properties and innervation of longitudinal and circular muscle layers of rabbit jejunum. , 1978, The Japanese journal of physiology.

[11]  M. A. Cook,et al.  Antagonism by methylxanthines of purine nucleotide-and dipyridamole-induced inhibition of peristaltic activity of the guinea pig ileum. , 1977, European journal of pharmacology.

[12]  E. Hayashi,et al.  Comparative studies on anti-nicotinic action of hexamethonium, mecamylamine and adenosine in the guinea pig isolated ileum. , 1977, Japanese Journal of Pharmacology.

[13]  E. Vizi,et al.  The inhibitory effect of adenosine and related nucleotides on the release of acetylcholine , 1976, Neuroscience.

[14]  H. Suzuki,et al.  Electrical and mechanical properties of longitudinal and circular muscles of the guinea-pig ileum. , 1975, The Japanese Journal of Physiology.

[15]  W. Paton,et al.  The origin of acetylcholine released from guinea‐pig intestine and longitudinal muscle strips , 1968, The Journal of physiology.

[16]  J. Harry,et al.  SOME PHARMACOLOGICAL PROPERTIES OF THE CIRCULAR AND LONGITUDINAL MUSCLE STRIPS FROM THE GUINEA-PIG ISOLATED ILEUM. , 1963, British journal of pharmacology and chemotherapy.

[17]  A. Frazer,et al.  THE NATURE OF THE ANTIPERISTALTIC FACTOR FROM WHEAT GLUTEN. , 1963, British journal of pharmacology and chemotherapy.

[18]  J. Harry.  The action of drugs on the circular muscle strip from the guinea-pig isolated ileum. , 1963, British journal of pharmacology and chemotherapy.