Analysis of Lipids in Green Coffee by Ultra-Performance Liquid Chromatography–Time-of-Flight Tandem Mass Spectrometry

Lipid components in green coffee were clarified to provide essential data support for green coffee processing. The types, components, and relative contents of lipids in green coffee were first analyzed by ultra-performance liquid chromatography–time-of-flight tandem mass spectrometry (UPLC-TOF-MS/MS). The results showed that the main fatty acids in green coffee were linoleic acid (43.39%), palmitic acid (36.57%), oleic acid (8.22%), and stearic acid (7.37%). Proportionally, the ratio of saturated fatty acids/unsaturated fatty acids/polyunsaturated fatty acids was close to 5.5:1:5.2. A total of 214 lipids were identified, including 15 sterols, 39 sphingosines, 12 free fatty acids, 127 glycerides, and 21 phospholipids. The main components of sterols, sphingosines, free fatty acids, glycerides, and phospholipids were acylhexosyl sitosterol, ceramide esterified omega-hydroxy fatty acid sphingosine, linoleic acid, and triglyceride, respectively. UPLC-TOF-MS/MS furnished high-quality and accurate information on TOF MS and TOF MS/MS spectra, providing a reliable analytical technology platform for analyzing lipid components in green coffee.

[1]  A. Paraskevopoulou,et al.  Single Origin Coffee Aroma: From Optimized Flavor Protocols and Coffee Customization to Instrumental Volatile Characterization and Chemometrics , 2021, Molecules.

[2]  Claudia Gonzalez Viejo,et al.  Integrating a Low-Cost Electronic Nose and Machine Learning Modelling to Assess Coffee Aroma Profile and Intensity , 2021, Sensors.

[3]  Fenghong Huang,et al.  Comprehensive and High-Coverage Lipidomic Analysis of Oilseeds Based on Ultrahigh-Performance Liquid Chromatography Coupled with Electrospray Ionization Quadrupole Time-of-Flight Mass Spectrometry. , 2021, Journal of agricultural and food chemistry.

[4]  Shen Xiao-jing,et al.  Advances on Chemical Components and Biological Activities of Coffee , 2021 .

[5]  Fang Wei,et al.  Ultrasound-assisted one-phase solvent extraction coupled with liquid chromatography-quadrupole time-of-flight mass spectrometry for efficient profiling of egg yolk lipids. , 2020, Food chemistry.

[6]  Y. Kitamura,et al.  Quantitative and Qualitative Evaluation of Fatty Acids in Coffee Oil and Coffee Residue , 2020 .

[7]  Xiaoqing Guo,et al.  Lipidomics analysis for identifying the geographical origin and lactation stage of goat milk. , 2019, Food chemistry.

[8]  Chang Zheng,et al.  Profiling and quantification of lipids in cold-pressed rapeseed oils based on direct infusion electrospray ionization tandem mass spectrometry. , 2019, Food chemistry.

[9]  Chun-Hui Zhang,et al.  Characterization and discrimination of selected China's domestic pork using an LC-MS-based lipidomics approach , 2019, Food Control.

[10]  H. El‐Seedi,et al.  A review on advanced microencapsulation technology to enhance bioavailability of phenolic compounds: Based on its activity in the treatment of Type 2 Diabetes , 2019, Trends in Food Science & Technology.

[11]  Marco Arlorio,et al.  Assessing fish authenticity by direct analysis in real time-high resolution mass spectrometry and multivariate analysis: discrimination between wild-type and farmed salmon. , 2019, Food research international.

[12]  Hehe Li,et al.  Comparative evaluation of the volatile profiles and taste properties of roasted coffee beans as affected by drying method and detected by electronic nose, electronic tongue, and HS-SPME-GC-MS. , 2019, Food chemistry.

[13]  Shaoquan Liu,et al.  Volatile composition and antioxidant capacity of Arabica coffee , 2013 .

[14]  Edgar Chambers,et al.  Evolution of sensory aroma attributes from coffee beans to brewed coffee. , 2011 .

[15]  Hong Zhang,et al.  Tracking phospholipid profiling of muscle from Ctennopharyngodon idellus during storage by shotgun lipidomics. , 2011, Journal of agricultural and food chemistry.

[16]  Felix Escher,et al.  Coffee roasting and aroma formation: application of different time-temperature conditions. , 2008, Journal of agricultural and food chemistry.