Fast detection of E. coli with a novel fluorescent biosensor based on a FRET system between UCNPs and GO@Fe3O4 in urine specimens.

Biosensors based on nanomaterials are becoming a research hotspot for the rapid detection of pathogenic bacteria. Herein, a "turn-on" fluorescent biosensor based on a FRET system was constructed for the fast detection of a representative pathogenic microorganism, namely, E. coli, which causes most urinary tract infections. This biosensor was constructed by utilizing synthesized UCNPs as fluorescent donors with stable luminescence performance in complex biological samples and GO@Fe3O4 as a receptor with both excellent adsorption ability and fluorescence quenching ability. A specific ssDNA selected as an aptamer which could recognize E. coli was immobilized on the UCNPs to form UCNP-Apt nanoprobes. The nanoprobes were adsorbed on the surface of GO@Fe3O4 through the π-stacking interactions between aptamers and GO. In the presence of E. coli, UCNP-Apt nanoprobes detached from GO@Fe3O4 due to the specific recognition of aptamers and bacteria, resulting in obvious fluorescence recovery, and the concentration of bacteria was positively correlated with the intensity of the fluorescence signal; such a "turn-on" signal output mode ensures excellent precision. In addition, the easy magnetic separation of GO@Fe3O4 simplifies the operation process, helping the sensor detect bacteria in 30 minutes with a linear range from 103 to 107 CFU mL-1 and a limit of detection of 467 CFU mL-1. Moreover, recovery test results also showed that the sensor has clinical application potential for the rapid detection of pathogenic microorganisms in complex biological samples.

[1]  A. Rajabzadeh,et al.  Upconverting nanoparticle clustering based rapid quantitative detection of tetrahydrocannabinol (THC) on lateral-flow immunoassay. , 2020, The Analyst.

[2]  M. S. El-shall,et al.  Highly fluorescent hematoporphyrin modified graphene oxide for selective detection of copper ions in aqueous solutions. , 2020, Analytica chimica acta.

[3]  Hans H. Gorris,et al.  Competitive upconversion-linked immunoassay using peptide mimetics for the detection of the mycotoxin zearalenone. , 2020, Biosensors & bioelectronics.

[4]  Shulin Zhao,et al.  Ratiometric fluorescent 3D DNA walker and catalyzed hairpin assembly for determination of microRNA , 2020, Microchimica Acta.

[5]  V. Stein,et al.  Ultrasensitive and Selective Copper(II) Detection: Introducing a Bioinspired and Robust Sensor , 2020, Chemistry.

[6]  Min Wei,et al.  A novel gold nanostars-based fluorescent aptasensor for aflatoxin B1 detection. , 2020, Talanta.

[7]  S. Hultgren,et al.  Urinary tract infections: microbial pathogenesis, host–pathogen interactions and new treatment strategies , 2020, Nature Reviews Microbiology.

[8]  M. Boissinot,et al.  Fast and Accurate Bacterial Species Identification in Urine Specimens Using LC-MS/MS Mass Spectrometry and Machine Learning. , 2019, Molecular & cellular proteomics : MCP.

[9]  Mahmoud Labib,et al.  Detection of pathogenic bacteria via nanomaterials-modified aptasensors. , 2019, Biosensors & bioelectronics.

[10]  Hui Chen,et al.  Fluorescent polymer dots and graphene oxide based nanocomplexes for "off-on" detection of metalloproteinase-9. , 2019, Nanoscale.

[11]  Sai Bi,et al.  Dual-Acceptor-Based Upconversion Luminescence Nanosensor with Enhanced Quenching Efficiency for in Situ Imaging and Quantification of MicroRNA in Living Cells. , 2019, ACS applied materials & interfaces.

[12]  G. Xie,et al.  A novel enzyme-free electrochemical biosensor for rapid detection of Pseudomonas aeruginosa based on high catalytic Cu-ZrMOF and conductive Super P. , 2019, Biosensors & bioelectronics.

[13]  Zhuo Chen,et al.  A Strategy of NIR Dual‐Excitation Upconversion for Ratiometric Intracellular Detection , 2019, Advanced science.

[14]  Sung-Yong Park,et al.  Rapid and in-situ detection of fecal indicator bacteria in water using simple DNA extraction and portable loop-mediated isothermal amplification (LAMP) PCR methods. , 2019, Water research.

[15]  S. M. Taghdisi,et al.  Fluorescent sensor for detection of miR-141 based on target-induced fluorescence enhancement and PicoGreen. , 2019, Talanta.

[16]  Cody S. Carrell,et al.  Rapid bacteria detection at low concentrations using sequential immunomagnetic separation and paper-based isotachophoresis. , 2019, Analytical chemistry.

[17]  Samaresh Das,et al.  Vancomycin functionalized WO3 thin film-based impedance sensor for efficient capture and highly selective detection of Gram-positive bacteria. , 2019, Biosensors & bioelectronics.

[18]  X. Qu,et al.  Remote and reversible control of in vivo bacteria clustering by NIR-driven multivalent upconverting nanosystems. , 2019, Biomaterials.

[19]  Jianding Qiu,et al.  Cobalt phosphide nanowires for fluorometric detection and in-situ imaging of telomerase activity via hybridization chain reactions , 2019, Microchimica Acta.

[20]  Ceyhun E. Kirimli,et al.  Rapid, label-free genetic detection of enteropathogens in stool without genetic isolation or amplification. , 2019, Biosensors & bioelectronics.

[21]  A. Wolff,et al.  Rapid detection of Salmonella enterica in food samples by a novel approach with combination of sample concentration and direct PCR. , 2019, Biosensors & bioelectronics.

[22]  J. Bergquist,et al.  Application of triple quadrupole mass spectrometry for the characterization of antibody–drug conjugates , 2019, Analytical and Bioanalytical Chemistry.

[23]  X. Guan,et al.  Rapid and sensitive detection of the activity of ADAM17 using a graphene oxide-based fluorescence sensor. , 2019, The Analyst.

[24]  Ki-Bum Lee,et al.  NIR Biosensing of Neurotransmitters in Stem Cell‐Derived Neural Interface Using Advanced Core–Shell Upconversion Nanoparticles , 2019, Advanced materials.

[25]  Mel S. Lee,et al.  Bacterial detection and identification from human synovial fluids on an integrated microfluidic system. , 2019, The Analyst.

[26]  T. Zhai,et al.  Simultaneous detection of telomerase and miRNA with graphene oxide-based fluorescent aptasensor in living cells and tissue samples. , 2019, Biosensors & bioelectronics.

[27]  Wei Xu,et al.  Detection and Inhibition of Bacteria on a Dual-Functional Silver Platform. , 2018, Small.

[28]  Qianhao Min,et al.  Lighting Up MicroRNA in Living Cells by the Disassembly of Lock-Like DNA-Programmed UCNPs-AuNPs through the Target Cycling Amplification Strategy. , 2018, Small.

[29]  G. Xie,et al.  Voltammetric immunoassay for Mycobacterium tuberculosis secretory protein MPT64 based on a synergistic amplification strategy using rolling circle amplification and a gold electrode modified with graphene oxide, Fe3O4 and Pt nanoparticles , 2018, Microchimica Acta.

[30]  S. Izumi,et al.  Rapid and sensitive detection of neuron specific enolase with a polydopamine coated plasmonic chip utilizing a rear-side coupling method. , 2018, The Analyst.

[31]  Zhenxin Wang,et al.  An upconversion nanoparticle-based fluorescence resonance energy transfer system for effectively sensing caspase-3 activity. , 2018, The Analyst.

[32]  Yi Lu,et al.  Upconversion Luminescence-Activated DNA Nanodevice for ATP Sensing in Living Cells. , 2018, Journal of the American Chemical Society.

[33]  M. Kostrzewa,et al.  Rapid detection of antibiotic resistance by MALDI-TOF mass spectrometry using a novel direct-on-target microdroplet growth assay. , 2017, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[34]  Huijun Zhao,et al.  Few‐Layer Graphdiyne Nanosheets Applied for Multiplexed Real‐Time DNA Detection , 2017, Advanced materials.

[35]  Erqun Song,et al.  Dual-Recognition Förster Resonance Energy Transfer Based Platform for One-Step Sensitive Detection of Pathogenic Bacteria Using Fluorescent Vancomycin-Gold Nanoclusters and Aptamer-Gold Nanoparticles. , 2017, Analytical chemistry.

[36]  J. Perry.  A Decade of Development of Chromogenic Culture Media for Clinical Microbiology in an Era of Molecular Diagnostics , 2017, Clinical Microbiology Reviews.

[37]  Yuchao Li,et al.  Single Upconversion Nanoparticle-Bacterium Cotrapping for Single-Bacterium Labeling and Analysis. , 2017, Small.

[38]  Bianca Sclavi,et al.  Rapid and accurate detection of Escherichia coli growth by fluorescent pH-sensitive organic nanoparticles for high-throughput screening applications. , 2016, Biosensors & bioelectronics.

[39]  Dayong Jin,et al.  Controlling upconversion nanocrystals for emerging applications. , 2015, Nature nanotechnology.