Multigenerational amplification of a reference ladder for alleles at locus D1S80.

The interlaboratory typing of DNA specimens that have been amplified at the D1S80 locus necessitates the use of a standard allelic reference ladder. This communication describes a technique in which individual, amplified alleles are isolated, combined, and amplified by PCR to produce a functional reference ladder composed of many of the alleles that occur at this locus. The amplified ladder can serve directly as a template source for production of the next generation of reference ladder. This process, in which each amplified ladder serves as the template for the next has been carried through multiple generations.

[1]  B. Budowle,et al.  Some Considerations for Use of AMP-FLPs for Identity Testing , 1992 .

[2]  A. Sajantila,et al.  Amplification of reproducible allele markers for amplified fragment length polymorphism analysis. , 1992, BioTechniques.

[3]  L. Peltonen,et al.  PCR amplification of alleles at the DIS80 locus: comparison of a Finnish and a North American Caucasian population sample, and forensic casework evaluation. , 1992, American journal of human genetics.

[4]  A. Sajantila,et al.  Identification of individuals with DNA testing. , 1991, Annals of medicine.

[5]  R. Dirnhofer,et al.  Typing of deoxyribonucleic acid (DNA) extracted from compact bone from human remains. , 1991, Journal of forensic sciences.

[6]  L. Peltonen,et al.  The polymerase chain reaction and post-mortem forensic identity testing: application of amplified D1S80 and HLA-DQ alpha loci to the identification of fire victims. , 1991, Forensic science international.

[7]  B Budowle,et al.  Fixed-bin analysis for statistical evaluation of continuous distributions of allelic data from VNTR loci, for use in forensic comparisons. , 1991, American journal of human genetics.

[8]  B Budowle,et al.  Analysis of the VNTR locus D1S80 by the PCR followed by high-resolution PAGE. , 1991, American journal of human genetics.

[9]  J. J. Kearney,et al.  Tracking the violent criminal offender through DNA typing profiles--a national database system concept. , 1991, EXS.

[10]  Y. Nakamura,et al.  Amplification of a variable number of tandem repeats (VNTR) locus (pMCT118) by the polymerase chain reaction (PCR) and its application to forensic science. , 1990, Journal of forensic sciences.

[11]  P. Marynen,et al.  Rapid detection of hypervariable regions by the polymerase chain reaction technique. , 1990, DNA and cell biology.

[12]  B. Budowle,et al.  Modifications to improve the effectiveness of restriction fragment length polymorphism typing. , 1990, Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society.

[13]  K. Klinger,et al.  Amplification of a highly polymorphic VNTR segment by the polymerase chain reaction. , 1989, Nucleic acids research.

[14]  B Budowle,et al.  A simple and sensitive method for quantifying human genomic DNA in forensic specimen extracts. , 1989, BioTechniques.

[15]  A. Jeffreys,et al.  Amplification of human minisatellites by the polymerase chain reaction: towards DNA fingerprinting of single cells. , 1988, Nucleic acids research.

[16]  Y. Nakamura,et al.  Isolation and mapping of a polymorphic DNA sequence (pMCT118) on chromosome 1p [D1S80]. , 1988, Nucleic acids research.

[17]  K. Mullis,et al.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. , 1988, Science.

[18]  K. Mullis,et al.  Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. , 1985, Science.