Transient accumulation of c-fos RNA following serum stimulation requires a conserved 5′ element and c-fos 3′ sequences

Transcription of the c-fos gene is transiently activated to generate large amounts of unstable c-fos RNA when quiescent fibroblasts are stimulated by polypeptide mitogens or whole serum. A cloned human c-fos gene (c-fosH) transfected into mouse fibroblasts is regulated in a similar manner. An element essential for transcription activation is located between nucleotides -332 and -276 relative to the mRNA cap site. This element has properties similar to those of previously characterized transcription enhancer elements. However, replacement of the 5' activating element by enhancers from SV40 or Moloney murine leukemia virus does not allow regulated c-fosH expression. The study of fusion genes showed that in addition to the 5' activating element, transient accumulation of c-fosH RNA following serum stimulation requires sequences at the 3' end of the c-fosH gene.

[1]  P. Leder,et al.  Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factor , 1983, Cell.

[2]  Brent H. Cochran,et al.  Molecular cloning of gene sequences regulated by platelet-derived growth factor , 1983, Cell.

[3]  E. Wagner,et al.  Differentiation of F9 teratocarcinoma stem cells after transfer of c-fos proto-oncogenes , 1984, Nature.

[4]  E. Chen,et al.  Supercoil sequencing: a fast method for sequencing plasmid DNA , 1985 .

[5]  R. Kamen,et al.  Polyomavirus enhancer contains multiple redundant sequence elements that activate both DNA replication and gene expression , 1985, Molecular and cellular biology.

[6]  T. Curran,et al.  c-fos protein can induce cellular transformation: A novel mechanism of activation of a cellular oncogene , 1984, Cell.

[7]  Tom Maniatis,et al.  Specific transcription and RNA splicing defects in five cloned β-thalassaemia genes , 1983, Nature.

[8]  D. Ish-Horowicz,et al.  Rapid and efficient cosmid cloning , 1981, Nucleic Acids Res..

[9]  R. Lerner,et al.  Nucleotide sequence of Moloney murine leukaemia virus , 1981, Nature.

[10]  S. Tonegawa,et al.  Cell type-specific enhancer element associated with a mouse MHC gene, Eβ , 1984, Nature.

[11]  Michael E. Greenberg,et al.  Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene , 1984, Nature.

[12]  H. Ruley,et al.  Sequence repeats in a polyoma virus DNA region important for gene expression , 1983, Journal of virology.

[13]  K. Yamamoto,et al.  DNA sequences bound specifically by glucocorticoid receptor in vitro render a heterologous promoter hormone responsive in vivo , 1983, Cell.

[14]  W. Schaffner,et al.  A small segment of polyoma virus DNA enhances the expression of a cloned beta-globin gene over a distance of 1400 base pairs. , 1981, Nucleic acids research.

[15]  T. Curran,et al.  Structure of the FBJ murine osteosarcoma virus genome: molecular cloning of its associated helper virus and the cellular homolog of the v-fos gene from mouse and human cells , 1983, Molecular and cellular biology.

[16]  M. Karin,et al.  Activation of a heterologous promoter in response to dexamethasone and cadmium by metallothionein gene 5′-flanking DNA , 1984, Cell.

[17]  R. Mulligan,et al.  Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[18]  R. Kamen,et al.  A region of the polyoma virus genome between the replication origin and late protein coding sequences is required in cis for both early gene expression and viral DNA replication. , 1981, Nucleic acids research.

[19]  R. Müller,et al.  Differential expression of c‐fos in hematopoietic cells: correlation with differentiation of monomyelocytic cells in vitro. , 1984, The EMBO journal.

[20]  D. Melton,et al.  Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. , 1984, Nucleic acids research.

[21]  W. Schaffner,et al.  An SV40 “enhancer trap” incorporates exogenous enhancers or generates enhancers from its own sequences , 1984, Cell.

[22]  M. Finkel,et al.  Virus Induction of osteosarcomas in Mice , 1966, Science.

[23]  J. Reiser,et al.  Three regions upstream from the cap site are required for efficient and accurate transcription of the rabbit β-globin gene in mouse 3T6 cells , 1983, Cell.

[24]  R. Ross,et al.  The platelet-derived growth factor , 1978, Cell.

[25]  I. Verma,et al.  Viral and cellular fos proteins: A comparative analysis , 1984, Cell.

[26]  T. Curran,et al.  Complete nucleotide sequence of a human c-onc gene: deduced amino acid sequence of the human c-fos protein. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[27]  E. Chen,et al.  Supercoil sequencing: a fast and simple method for sequencing plasmid DNA. , 1985, DNA.

[28]  R. Flavell,et al.  β + Thalassemia: Aberrant splicing results from a single point mutation in an intron , 1981, Cell.

[29]  H. Aviv,et al.  Messenger RNA population analysis during erythroid differentiation: a kinetical approach. , 1977, Journal of molecular biology.

[30]  P. Chambon,et al.  The SV40 72 base repair repeat has a striking effect on gene expression both in SV40 and other chimeric recombinants. , 1981, Nucleic acids research.

[31]  J. Vieira,et al.  The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. , 1982, Gene.

[32]  T. Maniatis,et al.  Identification of two distinct regulatory regions adjacent to the human β-interferon gene , 1983, Cell.

[33]  F. Sanger,et al.  DNA sequencing with chain-terminating inhibitors. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[34]  T. Curran,et al.  FBJ murine osteosarcoma virus: identification and molecular cloning of biologically active proviral DNA , 1982, Journal of virology.

[35]  I. Verma,et al.  Expression of c‐onc genes: c‐fos transcripts accumulate to high levels during development of mouse placenta, yolk sac and amnion. , 1983, The EMBO journal.

[36]  A. van der Eb,et al.  A new technique for the assay of infectivity of human adenovirus 5 DNA. , 1973, Virology.

[37]  I. Verma,et al.  Expression of the c-fos gene and of an fos-related gene is stimulated by platelet-derived growth factor. , 1984, Science.

[38]  R Staden,et al.  An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences. , 1982, Nucleic acids research.

[39]  R. Schreiber,et al.  Rapid induction of the expression of proto-oncogene fos during human monocytic differentiation , 1985, Cell.

[40]  P. Gruss,et al.  Activation of SV40 genome by 72-base pair tandem repeats of Moloney sarcoma virus , 1982, Nature.

[41]  Jonathan A. Cooper,et al.  Platelet-derived growth factor induces rapid but transient expression of the c-fos gene and protein , 1984, Nature.

[42]  T. Curran,et al.  Induction of c-fos gene and protein by growth factors precedes activation of c-myc , 1984, Nature.

[43]  J. Banerji,et al.  A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes , 1983, Cell.

[44]  W. Schaffner,et al.  Simian virus 40 enhancer increases RNA polymerase density within the linked gene , 1985, Nature.

[45]  T. Curran,et al.  Stimulation and inhibition of growth by EGF in different A431 cell clones is accompanied by the rapid induction of c‐fos and c‐myc proto‐oncogenes. , 1985, The EMBO journal.

[46]  S. Tonegawa,et al.  A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene , 1983, Cell.

[47]  Hans R. Schöler,et al.  Specific interaction between enhancer-containing molecules and cellular components , 1984, Cell.

[48]  J. Banerji,et al.  Expression of a β-globin gene is enhanced by remote SV40 DNA sequences , 1981, Cell.

[49]  R. Treisman,et al.  Simian virus 40 enhancer increases number of RNA polymerase II molecules on linked DNA , 1985, Nature.

[50]  G. Church,et al.  B lineage--specific interactions of an immunoglobulin enhancer with cellular factors in vivo. , 1985, Science.

[51]  S. Levy,et al.  Biosynthesis and stability of globin mRNA in cultured erythroleukemic friend cells , 1976, Cell.

[52]  Paolo Sassone-Corsi,et al.  A trans-acting factor is responsible for the simian virus 40 enhancer activity in vitro , 1985, Nature.

[53]  T. Curran,et al.  Induction of c-fos during myelomonocytic differentiation and macrophage proliferation , 1985, Nature.

[54]  T. Curran,et al.  Analysis of FBJ-MuSV provirus and c-fos (mouse) gene reveals that viral and cellular fos gene products have different carboxy termini , 1983, Cell.

[55]  C. Stiles.  The molecular biology of platelet-derived growth factor , 1983, Cell.

[56]  E. Adamson,et al.  Product of the cellular oncogene, c‐fos, observed in mouse and human tissues using an antibody to a synthetic peptide. , 1985, The EMBO journal.

[57]  B. Howard,et al.  Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells , 1982, Molecular and cellular biology.

[58]  T. Gonda,et al.  Expression of myb, myc and fos proto-oncogenes during the differentiation of a murine myeloid leukaemia , 1984, Nature.

[59]  M. Neuberger.  Expression and regulation of immunoglobulin heavy chain gene transfected into lymphoid cells. , 1983, The EMBO journal.

[60]  G. Church,et al.  Cell-type-specific contacts to immunoglobulin enhancers in nuclei , 1985, Nature.

[61]  P. Chambon,et al.  Stimulation of in vitro transcription from heterologous promoters by the simian virus 40 enhancer. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[62]  H. Pelham.  A regulatory upstream promoter element in the Drosophila Hsp 70 heat-shock gene , 1982, Cell.

[63]  W. Herr,et al.  Duplications of a mutated simian virus 40 enhancer restore its activity , 1985, Nature.

[64]  R. Treisman,et al.  Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping. , 1980, Methods in enzymology.

[65]  P. Gruss,et al.  Multiple point mutations affecting the simian virus 40 enhancer. , 1983, Science.