Intercellular bridges between granulosa cells and the oocyte in the elasmobranch Raya asterias

In the present ultrastructural study intercellular bridges, connecting somatic granulosa cells to oocyte, have been detected for the first time and their modifications have been followed during Raja oogenesis. Intercellular bridges make their first appearance in small previtellogenic follicles as connecting devices between small cells and the oocyte. Later on, when the follicular epithelium becomes polymorphic and multilayered, for the presence of small, large, and pyriform‐like cells, intercellular bridges link the oocyte and the different granulosa cells. Intercellular bridges contain ribosomes, whorl of membranes, mitochondria and vacuoles. Such cytoplasmic components are present also in the cell apex of large and pyriform‐like cells thus suggesting, in agreement with other species (Motta et al. J. Exp. Zool., 1996;276:223–241) they may flow toward the oocyte. In this regard the presence of intercellular bridges during the oogenesis of cartilagineous fish may represent a crucial event of the active cooperation between granulosa cells and the oocyte. Anat Rec 255:180–187, 1999. © 1999 Wiley‐Liss, Inc.

[1]  D. Paul,et al.  Female infertility in mice lacking connexin 37 , 1997, Nature.

[2]  T. Tricas,et al.  Periodic gonadal activity and protracted mating in elasmobranch fishes , 1996 .

[3]  P. Andreuccetti,et al.  Regression of the epithelium in late previtellogenic follicles of Podarcis sicula: A case of apoptosis , 1996 .

[4]  P. Andreuccetti,et al.  Role of pyriform cells during the growth of oocytes in the lizard Podarcis sicula , 1995 .

[5]  L. Cooley,et al.  Intercellular cytoplasm transport during Drosophila oogenesis. , 1994, Developmental biology.

[6]  P. Thomas,et al.  Ultrastructural changes in follicle cell-oocyte associations during development and maturation of the ovarian follicle in Atlantic croaker. , 1993, General and comparative endocrinology.

[7]  R. Wallace,et al.  Functional heterologous gap junctions in Fundulus ovarian follicles maintain meiotic arrest and permit hydration during oocyte maturation. , 1993, Developmental biology.

[8]  P. Andreuccetti.  An ultrastructural study of differentiatation of pyriform cells and their contribution to oocyte growth in representative squamata , 1992, Journal of morphology.

[9]  S. Minucci,et al.  Ultrastructural investigation of the corpora atretica of the electric ray, Torpedo marmorata. , 1992, General and comparative endocrinology.

[10]  M. Callebaut.  Pyriform-like and holding granulosa cells in the avian ovarian follicle wall , 1991 .

[11]  M. Callebaut.  Light- and electron-microscopic observations on the relationship between prelampbrush oocytes and surrounding granulosa cells in the laying Japanese quail (Coturnix coturnix japonica). , 1991, Reproduction, nutrition, development.

[12]  R. Wallace,et al.  Ultrastructural aspects of oogenesis and oocyte growth in fish and amphibians. , 1990, Journal of electron microscopy technique.

[13]  J. Eppig,et al.  Interactions between somatic cells and germ cells throughout mammalian oogenesis. , 1990, Biology of reproduction.

[14]  I. B. Wilson,et al.  Light and electron microscope studies on ovarian follicles in the lizard Chalcides ocellatus , 1989 .

[15]  S. Fasano,et al.  Reproductive biology of elasmobranchs with emphasis on endocrines , 1989 .

[16]  T. Iwamatsu,et al.  Oogenesis in the Medaka Oryzias latipes : Stages of Oocyte Development : Developmental Biology , 1988 .

[17]  L. Klosterman.  Ultrastructural and quantitative dynamics of the granulosa of ovarian follicles of the lizard gerrhonotus coeruleus (family anguidae) , 1987, Journal of morphology.

[18]  G. Chieffi,et al.  Regulation of Ovarian Steroidogenesis , 1987 .

[19]  H. W. Beams,et al.  The presence and distribution of gap junctions in the oocyte-follicle cell complex of the zebrafish, Brachydanio rerio. , 1985, Journal of submicroscopic cytology.

[20]  W. Kobayashi.  Communications of Oocyte‐Granulosa Cells in the Chum Salmon Ovary Detected by Transmission Electron Microscopy , 1985 .

[21]  W. Werner,et al.  Intercellular junctions between the follicle cells and oocytes of Xenopus laevis. , 1984, The Journal of experimental zoology.

[22]  J. G. Bluemink,et al.  The ultrastructural organization of gap junctions between follicle cells and the oocyte in Xenopus laevis. , 1984, European Journal of Cell Biology.

[23]  J. Eppig.  The relationship between cumulus cell-oocyte coupling, oocyte meiotic maturation, and cumulus expansion. , 1982, Developmental biology.

[24]  P. Andreuccetti,et al.  The differentiation and proliferation of follicle cells during oocyte growth in Lacerta sicula. , 1979, Journal of embryology and experimental morphology.

[25]  H S Wiley,et al.  Oocyte-follicle cell gap junctions in Xenopus laevis and the effects of gonadotropin on their permeability. , 1979, Science.

[26]  K. Toshimori,et al.  Gap junctions between microvilli of an oocyte and follicle cells in the teleost (Plecoglossus altivelis). , 1979, Zeitschrift fur mikroskopisch-anatomische Forschung.

[27]  P. Andreuccetti,et al.  Intercellular bridges between follicle cells and oocyte during the differentiation of follicular epithelium in Lacerta sicula Raf. , 1978, Journal of cell science.

[28]  N. Gilula,et al.  Cell-to-cell communication and ovulation. A study of the cumulus-oocyte complex , 1978, The Journal of cell biology.

[29]  D. Albertini,et al.  Gap junctions between the oocyte and companion follicle cells in the mammalian ovary , 1976, The Journal of cell biology.

[30]  H. R. Lindner,et al.  Organization of intramembrane particles in freeze-cleaved gap junctions of rat graafian rollicles: optical-diffraction analysis. , 1976, Journal of cell science.

[31]  R. D. Campbell.  Elimination by Hydra interstitial and nerve cells by means of colchicine. , 1976, Journal of cell science.

[32]  David F. Albertini,et al.  THE APPEARANCE AND STRUCTURE OF INTERCELLULAR CONNECTIONS DURING THE ONTOGENY OF THE RABBIT OVARIAN FOLLICLE WITH PARTICULAR REFERENCE TO GAP JUNCTIONS , 1974, The Journal of cell biology.

[33]  C. Taddei.  Significance of pyriform cells in ovarian follicle of Lacerta sicula. , 1972, Experimental cell research.

[34]  W. Neaves,et al.  Intercellular bridges between follicle cells and oocyte in the lizard, Anolis carolinensis , 1971, The Anatomical record.