Direct assay of tinidazole in human serum by micellar liquid chromatography.

A liquid chromatographic procedure for the direct determination of tinidazole in human serum is presented. It includes the use of a micellar mobile phase consisting of SDS (5.10(-2) M): propan-1-ol; (94:6, v/v) and a mu Bondapak CN column with UV detection at 320 nm. No solvent extraction or deproteinization are necessary. The linearity (0.1-10 mg L), the precision (3%), the reproducibility (1.3%), the recovery (99%), and the detection limit (0.1 mg L) in the tinidazole determination are comparable and sometimes greater than the corresponding tinidazole parameters when deproteinization and conventional reversed-phase HPLC are used. One hundred injections of serum samples do not affect the column life. The procedure is applied to ascertain the pharmacokinetics of 10 mg/kg of tinidazole.

[1]  P. Umapathi,et al.  Simultaneous Quantitative Determination of Tinidazole and Diloxanide Furoate in Tablet Preparations by Difference Spectroscopy , 1994 .

[2]  M. Guermouche,et al.  Direct determination of theophylline in human serum by high-performance liquid chromatography using zwitterionic micellar mobile phase. Comparison with an enzyme multiplied immunoassay technique. , 1993, The Analyst.

[3]  J. Eriksson,et al.  Rapid analysis of peptide toxins in cyanobacteria. , 1988, Journal of chromatography.

[4]  J. Haginaka,et al.  Retention properties of internal-surface reversed-phase silica packing and recovery of drugs from human plasma. , 1987, Journal of chromatography.

[5]  T. Pinkerton,et al.  Characterization of internal surface reversed-phase silica supports for liquid chromatography. , 1985, Journal of chromatography.

[6]  F. Deluccia,et al.  Direct serum injection with micellar liquid chromatography for therapeutic drug monitoring. , 1985, Analytical chemistry.

[7]  S. C. Bhatia,et al.  Electron-capture gas chromatographic assays of 5-nitroimidazole class of antimicrobials in blood. , 1984, Journal of chromatography.

[8]  D. Tonelli,et al.  Determination of metronidazole, misonidazole and its metabolite in serum and urine on RP-18 high-performance thin-layer chromatographic plates , 1981 .

[9]  F. Scharpf,et al.  Sensitive gas chromatographic assay of tinidazole in tissue and plasma. , 1979, Journal of chromatography.

[10]  N. Wood.  GLC analysis of metronidazole in human plasma. , 1975, Journal of pharmaceutical sciences.

[11]  E. Lau,et al.  Colorimetric determination of some N-1-substituted nitroimidazoles. , 1969, Journal of pharmaceutical sciences.

[12]  R. A. Hoodless,et al.  The polarographic determination of dimetridazole in animal feeding stuffs. , 1967, The Analyst.