Expression of β2-adrenergic Receptor mRNA in Peripheral Lung in Asthma and Chronic Obstructive Pulmonary Disease

Previous studies have indicated an increased number of β2-adrenergic receptors (β2AR) on bronchial smooth muscle in fatal asthma. This study evaluates the utility of autopsy lung for studies of gene expression and examines the hypothesis that increased expression of β2AR mRNA in peripheral lung underlies the increased receptor number reported in central airways in fatal asthma. β2AR mRNA levels have been quantitated using the ribonuclease protection assay on RNA from peripheral lung obtained both at autopsy and thoracotomy from subjects with normal lungs as well as subjects with asthma or chronic obstructive pulmonary disease (COPD). Glucocorticosteroid and serum induction of β2AR mRNA in human epidermoid carcinoma A431 cells, which display a high abundance of β2AR receptors, was also examined to provide aliquots of RNA containing relatively high levels of β2AR mRNA for use as positive controls and internal standards. In A431 cells maintained after confluence in serum-free media for 72 h, maximal β2AR mRN...

[1]  J. Hogg,et al.  Characterization of the inflammatory reaction in the peripheral airways of cigarette smokers using immunocytochemistry. , 1992, The American review of respiratory disease.

[2]  M. Caron,et al.  From ligand binding to gene expression: new insights into the regulation of G-protein-coupled receptors. , 1992, Trends in biochemical sciences.

[3]  J. Venter,et al.  Localization of beta 2-adrenoceptor messenger RNA in human and rat lung using in situ hybridization: correlation with receptor autoradiography. , 1991, European journal of pharmacology.

[4]  C. Malbon,et al.  The biology of beta-adrenergic receptors: analysis in human epidermoid carcinoma A431 cells. , 1991, The International journal of biochemistry.

[5]  J. Venter,et al.  Beta-adrenergic receptors. Relationship of primary structure, receptor function, and regulation. , 1990, The American review of respiratory disease.

[6]  J. Paterson,et al.  Autoradiographic localization of beta-adrenoceptors in asthmatic human lung. , 1989, The American review of respiratory disease.

[7]  C. Strader,et al.  Mapping the Functional Domains of the β-adrenergic Receptor , 1989 .

[8]  M. Caron,et al.  cAMP stimulates transcription of the beta 2-adrenergic receptor gene in response to short-term agonist exposure. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[9]  C. Malbon,et al.  Regulation of beta-adrenergic receptors by "permissive" hormones: glucocorticoids increase steady-state levels of receptor mRNA. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[10]  M. Caron,et al.  Beta-adrenergic receptors in hamster smooth muscle cells are transcriptionally regulated by glucocorticoids. , 1988, The Journal of biological chemistry.

[11]  C. Malbon,et al.  Down-regulation of beta-adrenergic receptors: agonist-induced reduction in receptor mRNA levels. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[12]  A. Strosberg,et al.  Structure of the gene for human beta 2-adrenergic receptor: expression and promoter characterization. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[13]  P. Chomczyński,et al.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. , 1987, Analytical biochemistry.

[14]  N. Costlow,et al.  A molecular titration assay to measure transcript prevalence levels. , 1987, Methods in enzymology.

[15]  R. Dixon,et al.  cDNA for the human beta 2-adrenergic receptor: a protein with multiple membrane-spanning domains and encoded by a gene whose chromosomal location is shared with that of the receptor for platelet-derived growth factor. , 1987, Proceedings of the National Academy of Sciences of the United States of America.