Four-dimensional in vivo X-ray microscopy with projection-guided gating

Visualizing fast micrometer scale internal movements of small animals is a key challenge for functional anatomy, physiology and biomechanics. We combine phase contrast tomographic microscopy (down to 3.3 μm voxel size) with retrospective, projection-based gating (in the order of hundreds of microseconds) to improve the spatiotemporal resolution by an order of magnitude over previous studies. We demonstrate our method by visualizing 20 three-dimensional snapshots through the 150 Hz oscillations of the blowfly flight motor.

[1]  M. Dickinson,et al.  In vivo length oscillations of indirect flight muscles in the fruit fly Drosophila virilis. , 1996, The Journal of experimental biology.

[2]  Wah-Keat Lee,et al.  Real-time phase-contrast x-ray imaging: a new technique for the study of animal form and function , 2007, BMC Biology.

[3]  Marco Stampanoni,et al.  Dose optimization approach to fast X-ray microtomography of the lung alveoli , 2013, Journal of applied crystallography.

[4]  A. P. Marchand.  Diamondoid Hydrocarbons--Delving into Nature's Bounty , 2003, Science.

[5]  S S Sagel,et al.  Gated computed tomography of the human heart. , 1977, Investigative radiology.

[6]  Marco Stampanoni,et al.  Fast reconstruction algorithm dealing with tomography artifacts , 2010, Optical Engineering + Applications.

[7]  S. Wilkins,et al.  Simultaneous phase and amplitude extraction from a single defocused image of a homogeneous object , 2002, Journal of microscopy.

[8]  T. Weitkamp,et al.  ANKAphase: software for single-distance phase retrieval from inline X-ray phase-contrast radiographs. , 2011, Journal of synchrotron radiation.

[9]  W. Kalender.  X-ray computed tomography , 2006, Physics in medicine and biology.

[10]  Kamel Fezzaa,et al.  Tracheal Respiration in Insects Visualized with Synchrotron X-ray Imaging , 2003, Science.

[11]  A. Rack,et al.  High-Speed X-ray Cineradiography for Analyzing Complex Kinematics in Living Insects , 2008 .

[12]  W. Danthanarayana,et al.  Insect Flight , 1986, Proceedings in Life Sciences.

[13]  Peter Modregger,et al.  Spatial resolution in Bragg‐magnified X‐ray images as determined by Fourier analysis , 2007 .

[14]  J W Hicks,et al.  Contribution of gular pumping to lung ventilation in monitor lizards. , 1999, Science.

[15]  Francesco De Carlo,et al.  Use of synchrotron tomography to image naturalistic anatomy in insects , 2008, Optical Engineering + Applications.

[16]  Maria Drangova,et al.  Least-error projection sorting to optimize retrospectively gated cardiac micro-CT of free-breathing mice. , 2012, Medical physics.

[17]  Maria Drangova,et al.  Fast Retrospectively Gated Quantitative Four-Dimensional (4D) Cardiac Micro Computed Tomography Imaging of Free-Breathing Mice , 2007, Investigative radiology.

[18]  G. E. Goslow,et al.  A Cineradiographic Analysis of Bird Flight: The Wishbone in Starlings Is a Spring , 1988, Science.

[19]  H. Krapp,et al.  In Vivo Time-Resolved Microtomography Reveals the Mechanics of the Blowfly Flight Motor , 2014, PLoS biology.

[20]  J. Socha,et al.  Visceral-Locomotory Pistoning in Crawling Caterpillars , 2010, Current Biology.

[21]  Stuart B Hooper,et al.  Synchrotron-based dynamic computed tomography of tissue motion for regional lung function measurement , 2012, Journal of The Royal Society Interface.

[22]  M. Stampanoni,et al.  Real Time Tomography at the Swiss Light Source , 2010 .

[23]  H. Krapp,et al.  Advantages of phase retrieval for fast x-ray tomographic microscopy , 2013 .

[24]  S M Bentzen,et al.  Evaluation of the spatial resolution of a CT scanner by direct analysis of the edge response function. , 1983, Medical physics.

[25]  Tomy dos Santos Rolo,et al.  In vivo X-ray cine-tomography for tracking morphological dynamics , 2014, Proceedings of the National Academy of Sciences.