论文信息 - Neuron imaging with Neurolucida--a PC-based system for image combining microscopy.

Neuron imaging with Neurolucida--a PC-based system for image combining microscopy.

Neurolucida is a new software package for performing 3-D neuron mapping and tracing to 0.5 micron precision through the oculars of a microscope. It incorporates computer controlled stage movement in three dimensions. Sections are limited in size only by the stage dimensions. An on-line, direct view image editor is provided as are dynamic image rotations in color and morphometric analyses. Neurolucida is written in 'C' and runs on PC ATs, XTs, and PS/2s using DOS and VGA, EGA, or NTSC graphics. Neurolucida has a video mode compatible with frame grabbers, thereby permitting its application to video microscopy.

[1] Edmund M. Glaser,et al. Snell's law: the bane of computer microscopists , 1982, Journal of Neuroscience Methods.

[2] C Levinthal,et al. Special techniques for the automatic computer reconstruction of neuronal structures. , 1980, Annual Review of Biophysics and Bioengineering.

[3] Edmund M. Glaser,et al. Analysis of thick brain sections by obverse—Reverse computer microscopy: Application of a new, high clarity Golgi—Nissl stain , 1981, Journal of Neuroscience Methods.

[4] Glaser Em,et al. Semiautomatic microscopy as a tool for cytologists. , 1986 .

[5] M. Freire,et al. An inexpensive and interactive microcomputer system for codifying golgi-impregnated neuronal morphology , 1986, Journal of Neuroscience Methods.

[6] Edmund M. Glaser,et al. The image-combining computer microscope — an interactive instrument for morphometry of the nervous system , 1983, Journal of Neuroscience Methods.

[7] James D. Foley,et al. Fundamentals of interactive computer graphics , 1982 .

[8] Peter Sterling,et al. A systematic approach to reconstructing microcircuitry by electron microscopy of serial sections , 1980, Brain Research Reviews.

[9] R. Ranney Mize,et al. The Microcomputer in Cell and Neurobiology Research , 1985 .