Application of membrane distillation for ethanol recovery during fuel ethanol production

Abstract The economic competitiveness of fuel ethanol in comparison with fossil fuels depends on the effective lowering of costs on each step of the process. One of the most promising methods seems to be the application of membrane separation technology. In this study, the focus is on improvement of brewer's wort fermentation. For this purpose, the membrane distillation process for ethanol recovery was implemented. The experimental system consisted of a bioreactor equipped with a capillary polypropylene microfiltration unit. Yeast cells’ count and viability, assimilation of sugars, production of ethanol and fermentation of by-products (glycerol and lactic acid) were monitored during fermentations. The intercellular trehalose as well as Hsp70 and Hsp104 heat shock proteins contents were determined. It was concluded that membrane distillation can be regarded as a straightforward method, which leads to an increase in ethanol production by facilitation of the continuous process, more complete fermentation of sugars, lowering the osmotic pressure in the fermentation broth, decreasing glycerol synthesis level and increasing yeast cells’ number and viability.

[1]  M. C. García-Payo,et al.  Air gap membrane distillation of aqueous alcohol solutions , 2000 .

[2]  Takeshi Matsuura,et al.  Synthetic Membranes and Membrane Separation Processes , 1993 .

[3]  G. Walker,et al.  Physiological and Molecular Responses of Yeasts to the Environment , 2006 .

[4]  K. Smart,et al.  The Osmotic Stress Response of Ale and Lager Brewing Yeast Strains , 2008 .

[5]  M. Gryta,et al.  The influence of polypropylene degradation on the membrane wettability during membrane distillation , 2009 .

[6]  M. Gryta The fermentation process integrated with membrane distillation , 2001 .

[7]  H. Kataoka,et al.  Ethanol stripping by pervaporation using porous PTFE membrane , 1987 .

[8]  T M Swan,et al.  Stress tolerance in a yeast sterol auxotroph: role of ergosterol, heat shock proteins and trehalose. , 1998, FEMS microbiology letters.

[9]  H. Bohnert,et al.  Roles of sugar alcohols in osmotic stress adaptation. Replacement of glycerol by mannitol and sorbitol in yeast. , 1999, Plant physiology.

[10]  K. Verstrepen,et al.  Glucose and sucrose: hazardous fast-food for industrial yeast? , 2004, Trends in biotechnology.

[11]  Kai Sundmacher,et al.  Experimental investigation on a membrane distillation based micro-separator , 2010 .

[12]  S. Dequin,et al.  Stress effect of ethanol on fermentation kinetics by stationary-phase cells of Saccharomyces cerevisiae , 2001, Biotechnology Letters.

[13]  Antoni W. Morawski,et al.  Ethanol production in membrane distillation bioreactor , 2000 .

[14]  Gunnar Eigil Jonsson,et al.  Factors affecting flux and ethanol separation performance in vacuum membrane distillation (VMD) , 2003 .

[15]  Freddy R. Delvaux,et al.  Isolation and Characterization of Brewer's Yeast Variants with Improved Fermentation Performance under High-Gravity Conditions , 2006, Applied and Environmental Microbiology.

[16]  U. von Stockar,et al.  Extractive fermentation of ethanol using membrane distillation , 1989, Biotechnology Letters.

[17]  Won Hi Hong,et al.  Effect of operating variables on the flux and selectivity in sweep gas membrane distillation for dilute aqueous isopropanol , 2001 .

[18]  M.H.V. Mulder,et al.  ETHANOL-WATER SEPARATION BY MEMBRANE DISTILLATION: EFFECT OF TEMPERATURE POLARIZATION , 1987 .

[19]  Rajni Hatti-Kaul,et al.  Downstream Processing in Industrial Biotechnology , 2010 .

[20]  P. Piper The heat shock and ethanol stress responses of yeast exhibit extensive similarity and functional overlap. , 1995, FEMS microbiology letters.

[21]  W. Brandt,et al.  The yeast Saccharomyces cerevisiae stress response protein Hsp12p decreases the gel strength of agarose used as a model system for the β-glucan layer of the cell wall , 2005 .

[22]  Huajiang Huang,et al.  A review of separation technologies in current and future biorefineries , 2008 .

[23]  A. Panek,et al.  Trehalose metabolism in Saccharomyces cerevisiae during alcoholic fermentation , 1997, Biotechnology Letters.

[24]  K. Luyben,et al.  Ethanol production in an integrated fermentation/membrane system. Process simulations and economics , 1993 .

[25]  Fawzi Banat,et al.  Modeling of dilute ethanol-water mixture separation by membrane distillation , 1999 .

[26]  Kaseno,et al.  Effect of Product Removal by a Pervaporation on Ethanol Fermentation , 1998 .

[27]  D. Pejin,et al.  Bioethanol production from corn meal by simultaneous enzymatic saccharification and fermentation with immobilized cells of Saccharomyces cerevisiae var. ellipsoideus , 2009 .

[28]  García-Payo,et al.  Wetting Study of Hydrophobic Membranes via Liquid Entry Pressure Measurements with Aqueous Alcohol Solutions. , 2000, Journal of colloid and interface science.

[29]  J. C. Craig,et al.  Ethanol production in a continuous fermentation/membrane pervaporation system , 1996, Applied Microbiology and Biotechnology.

[30]  A. Peña,et al.  Comparative analysis of trehalose production by Debaryomyces hansenii and Saccharomyces cerevisiae under saline stress , 2005, Extremophiles.

[31]  Siddhartha Datta,et al.  Analysis of the performance of a continuous membrane bioreactor with cell recycling during ethanol fermentation , 1998 .