Development of Decellularized Cornea by Organic Acid Treatment for Corneal Regeneration.

IMPACT STATEMENT This study successfully developed decellularized corneal scaffolds that were prepared by organic acid, which safely exist in animal tissues and plants. The results showed the highly efficient removal of cell debris from porcine corneas, and excellent preservation of optical properties, extracellular matrix (ECM) architecture, and biomolecules. In addition, decellularized corneal scaffolds revealed excellent biocompatibility and recellularization potential in vitro. In an animal model, the transplanted corneas were completely epithelialized, clear, showed no signs of immune response, and effectively supported stromal keratocytes growth. Hence, this could be a promising scaffold material for corneal tissue engineering applications.

[1]  M. Yeh,et al.  Preparation of acellular scaffold for corneal tissue engineering by supercritical carbon dioxide extraction technology. , 2017, Acta biomaterialia.

[2]  Yan Jin,et al.  Construction of tissue-engineered cornea composed of amniotic epithelial cells and acellular porcine cornea for treating corneal alkali burn. , 2013, Biomaterials.

[3]  G. Khang,et al.  Nature-Derived Aloe Vera Gel Blended Silk Fibroin Film Scaffolds for Cornea Endothelial Cell Regeneration and Transplantation. , 2016, ACS applied materials & interfaces.

[4]  D. Cooper,et al.  Selected physiologic compatibilities and incompatibilities between human and porcine organ systems , 2006, Xenotransplantation.

[5]  A. Hopkinson,et al.  Corneal Decellularization: A Method of Recycling Unsuitable Donor Tissue for Clinical Translation? , 2015, Current eye research.

[6]  D. Maurice.  The structure and transparency of the cornea , 1957, The Journal of physiology.

[7]  J. Mehta,et al.  Management of corneal perforation. , 2011, Survey of ophthalmology.

[8]  Jorge L Alio,et al.  Acellular human corneal matrix sheets seeded with human adipose-derived mesenchymal stem cells integrate functionally in an experimental animal model. , 2015, Experimental eye research.

[9]  Stephen F Badylak,et al.  An overview of tissue and whole organ decellularization processes. , 2011, Biomaterials.

[10]  Gilles Thuret,et al.  Global Survey of Corneal Transplantation and Eye Banking. , 2016, JAMA ophthalmology.

[11]  S. Badylak,et al.  The effect of detergents on the basement membrane complex of a biologic scaffold material. , 2014, Acta biomaterialia.

[12]  V. Carriel,et al.  Effects of Detergent-Based Protocols on Decellularization of Corneas With Sclerocorneal Limbus. Evaluation of Regional Differences. , 2015, Translational vision science & technology.

[13]  Mark Ahearne,et al.  Strategies for developing decellularized corneal scaffolds. , 2013, Experimental eye research.

[14]  Seiichi Funamoto,et al.  Preparation and characterization of decellularized cornea using high-hydrostatic pressurization for corneal tissue engineering. , 2010, Biomaterials.

[15]  Thomas W Gilbert,et al.  Strategies for tissue and organ decellularization , 2012, Journal of cellular biochemistry.

[16]  J. Forrester,et al.  Immunological responses in mice to full-thickness corneal grafts engineered from porcine collagen. , 2007, Biomaterials.

[17]  J. Kohn,et al.  Development and Characterization of Acellular Extracellular Matrix Scaffolds from Porcine Menisci for Use in Cartilage Tissue Engineering. , 2015, Tissue engineering. Part C, Methods.

[18]  H. Mirzadeh,et al.  Novel materials to enhance corneal epithelial cell migration on keratoprosthesis , 2010, British Journal of Ophthalmology.

[19]  Drew M. Pardoll,et al.  Design, clinical translation and immunological response of biomaterials in regenerative medicine , 2016 .

[20]  Fang Wu,et al.  Construction of a Corneal Stromal Equivalent with SMILE-Derived Lenticules and Fibrin Glue , 2016, Scientific Reports.

[21]  A Pizzoferrato,et al.  Cell culture methods for testing biocompatibility. , 1994, Clinical materials.

[22]  R. Gemeinhart,et al.  Decellularized human cornea for reconstructing the corneal epithelium and anterior stroma. , 2012, Tissue engineering. Part C, Methods.

[23]  J. Mehta,et al.  Decellularization of human stromal refractive lenticules for corneal tissue engineering , 2016, Scientific Reports.

[24]  F. O'Brien,et al.  Understanding the effect of mean pore size on cell activity in collagen-glycosaminoglycan scaffolds , 2010, Cell adhesion & migration.

[25]  Yongchun Meng,et al.  Cross-linked decellularized porcine corneal graft for treating fungal keratitis , 2017, Scientific Reports.

[26]  Zuguo Liu,et al.  An Ultra-thin Amniotic Membrane as Carrier in Corneal Epithelium Tissue-Engineering , 2015, Scientific Reports.

[27]  L. Griffiths,et al.  Immunogenicity in xenogeneic scaffold generation: antigen removal vs. decellularization. , 2014, Acta biomaterialia.