Gonadotropin-releasing hormone (GnRH) in pituitary stalk blood from proestrous rats: effects of anesthetics and relationship between stored and released GnRH and luteinizing hormone.

We have studied the effects of various anesthetics on the spontaneous release of gonadotropin-releasing hormone (GnRH) into hypophysial portal vessel blood and used the techniques of prolonged electrical stimulation of the hypothalamus and iv infusion of GnRH to determine more precisely the relationships between GnRH and LH release and between stored hormone and the amount released. A surge of GnRH (measured by RIA) occurred at the expected time of the spontaneous surge of plasma LH in rats anesthetized on proestrus with Althesin (alphaxalone and alphadolone acetate) but not in rats anesthetized with α-chloralose, ketamine hydrochloride, or urethane. The average amount of GnRH released into portal blood during the surge (128 pg) was relatively small compared to the amount stored in the hypothalamus (6–8 ng). Electrical stimulation of the medial preoptic area in rats anesthetized with urethane produced a surge of LH that was similar to the spontaneous LH surge. This induced surge of LH was preceded by a re...

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