Strains differentiation of Microsporum canis by RAPD analysis using (GACA)4 and (ACA)5 primers.

Molecular analysis of dermatophytes (based on PCR fingerprinting) revealed high clonal differentiation between the genus and species. Microsporum canis (zoophilic dermatophyte, belonging to genus Microsporum), responsible for most cases of tinea capitis in children, tinea corporis in adults and dermatophytoses in cats, is very unique in comparison with other dermatophytes. Results of most molecular studies show that there is no clonal differentiation within M. canis as distinct from other species. The aim of this study was application of (GACA)4 repetitive primer and (ACA)5 primer for typing of M. canis strains isolated from human and animals in Central Poland. Fungal strains: 32 clinical isolates of M. canis, originated from patients from Central Poland; 11 strains isolated from infected cats (6) and dogs (7), reference strains of M. canis (CBS 113480), T rubrum (CBS 120358), T mentagrophytes (CBS 120357) and E. floccosum (CBS 970.95). The genomic DNAs of the strains were used as a template in RAPD reaction. No differentiation was observed for the analyzed M. canis strains using (GACA)4 and (ACA)5 typing.

[1]  Justyna Leibner-Ciszak,et al.  Evaluation of a PCR melting profile method for intraspecies differentiation of Trichophyton rubrum and Trichophyton interdigitale. , 2010, Journal of medical microbiology.

[2]  S. de Hoog,et al.  A virulent genotype of Microsporum canis is responsible for the majority of human infections. , 2007, Journal of medical microbiology.

[3]  P. Stączek,et al.  PCR-RFLP analysis of the dermatophytes isolated from patients in Central Poland. , 2006, Journal of dermatological science.

[4]  J. Guarro,et al.  Inter-single-sequence-repeat-PCR typing as a new tool for identification of Microsporum canis strains. , 2005, Journal of dermatological science.

[5]  R. Cordeiro,et al.  Antifungal susceptibility and genotypical pattern of Microsporum canis strains. , 2005, Canadian journal of microbiology.

[6]  G. S. de Hoog,et al.  Population structure and evolutionary origins of Microsporum canis, M. ferrugineum and M. audouinii. , 2004, Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases.

[7]  S. Kelly,et al.  Restriction Fragment Length Polymorphism Analysis of Ribosomal DNA Intergenic Regions Is Useful for Differentiating Strains of Trichophyton mentagrophytes , 2003, Journal of Clinical Microbiology.

[8]  C. Jackson,et al.  Molecular strain typing of Trichophyton rubrum indicates multiple strain involvement in onychomycosis , 2003, The British journal of dermatology.

[9]  E. Difonzo,et al.  Application of PCR to Distinguish Common Species of Dermatophytes , 2001, Journal of Clinical Microbiology.

[10]  G. S. de Hoog,et al.  Molecular Taxonomy of the Trichophyton rubrum Complex , 2000, Journal of Clinical Microbiology.

[11]  R. Baird,et al.  Rapid Mini-Preparation of Fungal DNA for PCR , 2000, Journal of Clinical Microbiology.

[12]  C. Jackson,et al.  Species Identification and Strain Differentiation of Dermatophyte Fungi by Analysis of Ribosomal-DNA Intergenic Spacer Regions , 1999, Journal of Clinical Microbiology.

[13]  M. Uehara,et al.  Random amplification of polymorphic DNA is useful for the differentiation of several anthropophilic dermatophytes , 1997, Mycoses.

[14]  T. G. Mitchell,et al.  Identification of pathogenic yeasts of the imperfect genus Candida by polymerase chain reaction fingerprinting , 1997, Electrophoresis.

[15]  T. G. Mitchell,et al.  Hybridization probes for conventional DNA fingerprinting used as single primers in the polymerase chain reaction to distinguish strains of Cryptococcus neoformans , 1993, Journal of clinical microbiology.

[16]  D. Ellsworth,et al.  Artifactual variation in randomly amplified polymorphic DNA banding patterns. , 1993, BioTechniques.