Graphite nanoplatelets and Caenorhabditis elegans: insights from an in vivo model.

We evaluated the toxicity of graphite nanoplatelets (GNPs) in the model organism Caenorhabditis elegans. The GNPs resulted nontoxic by measuring longevity as well as reproductive capability end points. An imaging technique based on Fourier transform infrared spectroscopy (FT-IR) mapping was also developed to analyze the GNPs spatial distribution inside the nematodes. Conflicting reports on the in vitro antimicrobial properties of graphene-based nanomaterials prompted us to challenge the host-pathogen system C. elegans-Pseudomonas aeruginosa to assess these findings through an in vivo model.

[1]  Nora Khanarian,et al.  In vivo and scanning electron microscopy imaging of up-converting nanophosphors in Caenorhabditis elegans. , 2006, Nano letters.

[2]  S. Brenner.  The genetics of Caenorhabditis elegans. , 1974, Genetics.

[3]  O. Zugasti,et al.  C. elegans: model host and tool for antimicrobial drug discovery , 2011, Disease Models & Mechanisms.

[4]  G. Fudenberg,et al.  Ultrahigh electron mobility in suspended graphene , 2008, 0802.2389.

[5]  Andre K. Geim,et al.  Electric Field Effect in Atomically Thin Carbon Films , 2004, Science.

[6]  M. S. Sarto,et al.  Electromagnetic properties of composites containing graphite nanoplatelets at radio frequency , 2011 .

[7]  Gerald B. Pier,et al.  Lung Infections Associated with Cystic Fibrosis , 2002, Clinical Microbiology Reviews.

[8]  Pinaki Sengupta,et al.  Synthesis of silver nanoparticles in an aqueous suspension of graphene oxide sheets and its antimicrobial activity. , 2011, Colloids and surfaces. B, Biointerfaces.

[9]  S. Doglia,et al.  Tetracycline and its analogues protect Caenorhabditis elegans from β amyloid-induced toxicity by targeting oligomers , 2010, Neurobiology of Disease.

[10]  Dun Zhang,et al.  Synthesis and characterization of silver nanoparticle and graphene oxide nanosheet composites as a bactericidal agent for water disinfection. , 2011, Journal of colloid and interface science.

[11]  Oscar N. Ruiz,et al.  Graphene oxide: a nonspecific enhancer of cellular growth. , 2011, ACS nano.

[12]  Ken Donaldson,et al.  Graphene-based nanoplatelets: a new risk to the respiratory system as a consequence of their unusual aerodynamic properties. , 2012, ACS nano.

[13]  Yanli Chang,et al.  In vitro toxicity evaluation of graphene oxide on A549 cells. , 2011, Toxicology letters.

[14]  P. Wallace.  The Band Theory of Graphite , 1947 .

[15]  R. Piner,et al.  Biocompatible, Robust Free‐Standing Paper Composed of a TWEEN/Graphene Composite , 2010, Advanced materials.

[16]  D. Barra,et al.  Anti-Pseudomonas Activity of Frog Skin Antimicrobial Peptides in a Caenorhabditis elegans Infection Model: a Plausible Mode of Action In Vitro and In Vivo , 2010, Antimicrobial Agents and Chemotherapy.

[17]  Z. Marković,et al.  In vitro comparison of the photothermal anticancer activity of graphene nanoparticles and carbon nanotubes. , 2011, Biomaterials.

[18]  Huan-Cheng Chang,et al.  In vivo imaging and toxicity assessments of fluorescent nanodiamonds in Caenorhabditis elegans. , 2010, Nano letters.

[19]  Jongheop Yi,et al.  Ecotoxicity of silver nanoparticles on the soil nematode Caenorhabditis elegans using functional ecotoxicogenomics. , 2009, Environmental science & technology.

[20]  Omid Akhavan,et al.  Toxicity of graphene and graphene oxide nanowalls against bacteria. , 2010, ACS nano.

[21]  J. Lyczak,et al.  Establishment of Pseudomonas aeruginosa infection: lessons from a versatile opportunist. , 2000, Microbes and infection.

[22]  Liangzhu Feng,et al.  Graphene in biomedicine: opportunities and challenges. , 2011, Nanomedicine.

[23]  G. Lucovsky,et al.  Infrared active optical vibrations of graphite , 1977 .

[24]  A. von Mikecz,et al.  In Caenorhabditis elegans Nanoparticle-Bio-Interactions Become Transparent: Silica-Nanoparticles Induce Reproductive Senescence , 2009, PloS one.

[25]  Shin Sik Choi,et al.  Apoptosis-mediated in vivo toxicity of hydroxylated fullerene nanoparticles in soil nematode Caenorhabditis elegans. , 2012, Chemosphere.

[26]  Klaus Kern,et al.  Atomic hole doping of graphene. , 2008, Nano letters.

[27]  Mark C Hersam,et al.  Minimizing oxidation and stable nanoscale dispersion improves the biocompatibility of graphene in the lung. , 2011, Nano letters.

[28]  S. Doglia,et al.  Fourier transform infrared microspectroscopy as a new tool for nematode studies , 2004, FEBS letters.

[29]  Yuehe Lin,et al.  Graphene and graphene oxide: biofunctionalization and applications in biotechnology , 2011, Trends in Biotechnology.

[30]  C. N. Lau,et al.  Superior thermal conductivity of single-layer graphene. , 2008, Nano letters.

[31]  D. Sattelle,et al.  A role for Caenorhabditis elegans in understanding the function and interactions of human disease genes. , 2000, Human molecular genetics.