Identification of Lactaldehyde Dehydrogenase in Methanocaldococcus jannaschii and Its Involvement in Production of Lactate for F420 Biosynthesis

ABSTRACT One of the early steps in the biosynthesis of coenzyme F420 in Methanocaldococcus jannaschii requires generation of 2-phospho-l-lactate, which is formed by the phosphorylation of l-lactate. Preliminary studies had shown that l-lactate in M. jannaschii is not derived from pyruvate, and thus an alternate pathway(s) for its formation was examined. Here we report that l-lactate is formed by the NAD+-dependent oxidation of l-lactaldehyde by the MJ1411 gene product. The lactaldehyde, in turn, was found to be generated either by the NAD(P)H reduction of methylglyoxal or by the aldol cleavage of fuculose-1-phosphate by fuculose-1-phosphate aldolase, the MJ1418 gene product.

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