3D fiber-deposited scaffolds for tissue engineering: influence of pores geometry and architecture on dynamic mechanical properties.

One of the main issues in tissue engineering is the fabrication of scaffolds that closely mimic the biomechanical properties of the tissues to be regenerated. Conventional fabrication techniques are not sufficiently suitable to control scaffold structure to modulate mechanical properties. Within novel scaffold fabrication processes 3D fiber deposition (3DF) showed great potential for tissue engineering applications because of the precision in making reproducible 3D scaffolds, characterized by 100% interconnected pores with different shapes and sizes. Evidently, these features also affect mechanical properties. Therefore, in this study we considered the influence of different structures on dynamic mechanical properties of 3DF scaffolds. Pores were varied in size and shape, by changing fibre diameter, spacing and orientation, and layer thickness. With increasing porosity, dynamic mechanical analysis (DMA) revealed a decrease in elastic properties such as dynamic stiffness and equilibrium modulus, and an increase of the viscous parameters like damping factor and creep unrecovered strain. Furthermore, the Poisson's ratio was measured, and the shear modulus computed from it. Scaffolds showed an adaptable degree of compressibility between sponges and incompressible materials. As comparison, bovine cartilage was tested and its properties fell in the fabricated scaffolds range. This investigation showed that viscoelastic properties of 3DF scaffolds could be modulated to accomplish mechanical requirements for tailored tissue engineered applications.

[1]  M. B. Claase,et al.  Gas plasma etching of PEO/PBT segmented block copolymer films. , 2003, Journal of biomedical materials research. Part A.

[2]  C. V. van Blitterswijk,et al.  The effect of PEGT/PBT scaffold architecture on the composition of tissue engineered cartilage. , 2005, Biomaterials.

[3]  S. Bulstra,et al.  FEMORAL CANAL OCCLUSION IN TOTAL HIP REPLACEMENT USING A RESORBABLE AND FLEXIBLE CEMENT RESTRICTOR , 1996 .

[4]  M. B. Claase,et al.  Design of segmented poly(ether ester) materials and structures for the tissue engineering of bone. , 2002, Journal of controlled release : official journal of the Controlled Release Society.

[5]  Robert E Guldberg,et al.  Microarchitectural and mechanical characterization of oriented porous polymer scaffolds. , 2003, Biomaterials.

[6]  E. Carew,et al.  The Effect of Strain Rate on the Viscoelastic Response of Aortic Valve Tissue: A Direct-Fit Approach , 2004, Annals of Biomedical Engineering.

[7]  A Ahluwalia,et al.  Microsyringe-based deposition of two-dimensional and three-dimensional polymer scaffolds with a well-defined geometry for application to tissue engineering. , 2002, Tissue engineering.

[8]  K. Menard.  Dynamic Mechanical Analysis: A Practical Introduction , 1997 .

[9]  Malcolm N. Cooke,et al.  Use of stereolithography to manufacture critical-sized 3D biodegradable scaffolds for bone ingrowth. , 2003, Journal of biomedical materials research. Part B, Applied biomaterials.

[10]  K. Leong,et al.  The design of scaffolds for use in tissue engineering. Part II. Rapid prototyping techniques. , 2002, Tissue engineering.

[11]  M. Kellomäki,et al.  Dynamic mechanical characterization of biodegradable composites of hydroxyapatite and polylactides. , 2001, Journal of biomedical materials research.

[12]  M. Cima,et al.  Mechanical properties of dense polylactic acid structures fabricated by three dimensional printing. , 1996, Journal of biomaterials science. Polymer edition.

[13]  C. Du,et al.  Biomimetic calcium phosphate coatings on Polyactive 1000/70/30. , 2002, Journal of biomedical materials research.

[14]  A Ratcliffe,et al.  Cartilage and diarthrodial joints as paradigms for hierarchical materials and structures. , 1992, Biomaterials.

[15]  T. Meyvis,et al.  A comparison between the use of dynamic mechanical analysis and oscillatory shear rheometry for the characterisation of hydrogels. , 2002, International journal of pharmaceutics.

[16]  J. J. Grote,et al.  Effect of implantation site on phagocyte/polymer interaction and fibrous capsule formation. , 1988, Biomaterials.

[17]  R. Langer,et al.  Adhesion-mediated signal transduction in human articular chondrocytes: the influence of biomaterial chemistry and tenascin-C. , 2004, Experimental cell research.

[18]  M. Spector,et al.  Rheology of joint fluid in total knee arthroplasty patients , 2002, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[19]  Rainer Schmelzeisen,et al.  Fabrication of soft tissue engineering scaffolds by means of rapid prototyping techniques , 2002 .

[20]  C. V. van Blitterswijk,et al.  Biocompatibility of a biodegradable matrix used as a skin substitute: an in vivo evaluation. , 1994, Journal of biomedical materials research.

[21]  R. Landers,et al.  Rapid prototyping of scaffolds derived from thermoreversible hydrogels and tailored for applications in tissue engineering. , 2002, Biomaterials.

[22]  Selim Elhadj,et al.  Implementation of an optical method for the real-time determination of uniaxial strain and vessel mechanics , 2004, IEEE Transactions on Biomedical Engineering.

[23]  C. V. van Blitterswijk,et al.  Design of porous scaffolds for cartilage tissue engineering using a three-dimensional fiber-deposition technique. , 2004, Biomaterials.

[24]  J. Riesle,et al.  Effectiveness and Safety of the PEGT/PBT Copolymer Scaffold as Dermal Substitute in Scar Reconstruction Wounds (Feasibility Trial) , 2004, Cell and Tissue Banking.

[25]  D. Jones,et al.  Dynamic mechanical analysis of polymeric systems of pharmaceutical and biomedical significance. , 1999, International journal of pharmaceutics.

[26]  D. Fyhrie,et al.  Apparent viscoelastic anisotropy as measured from nondestructive oscillatory tests can reflect the presence of a flaw in cortical bone. , 2004, Journal of biomedical materials research. Part A.

[27]  Scott C. Brown,et al.  A three-dimensional osteochondral composite scaffold for articular cartilage repair. , 2002, Biomaterials.

[28]  J. Feijen,et al.  Control of vitamin B12 release from poly(ethylene glycol)/poly(butylene terephthalate) multiblock copolymers. , 2002, Biomaterials.

[29]  L G Griffith,et al.  Survival and function of hepatocytes on a novel three-dimensional synthetic biodegradable polymer scaffold with an intrinsic network of channels. , 1998, Annals of surgery.

[30]  D. Hutmacher,et al.  Scaffolds in tissue engineering bone and cartilage. , 2000, Biomaterials.

[31]  J. Feijen,et al.  A controlled release system for proteins based on poly(ether ester) block-copolymers: polymer network characterization. , 1999, Journal of controlled release : official journal of the Controlled Release Society.

[32]  A. Ahluwalia,et al.  Fabrication of PLGA scaffolds using soft lithography and microsyringe deposition. , 2003, Biomaterials.

[33]  T B F Woodfield,et al.  Scaffolds for tissue engineering of cartilage. , 2002, Critical reviews in eukaryotic gene expression.

[34]  I. Zein,et al.  Fused deposition modeling of novel scaffold architectures for tissue engineering applications. , 2002, Biomaterials.

[35]  G. Lewis.  Key issues involved with the use of miniature specimens in the characterization of the mechanical behavior of polymeric biomaterials--a review. , 2002, Journal of Biomedical Materials Research.

[36]  I Zein,et al.  Mechanical properties and cell cultural response of polycaprolactone scaffolds designed and fabricated via fused deposition modeling. , 2001, Journal of biomedical materials research.

[37]  E B Hunziker,et al.  Optical and mechanical determination of Poisson's ratio of adult bovine humeral articular cartilage. , 1997, Journal of biomechanics.

[38]  R K Korhonen,et al.  Biomechanical properties of knee articular cartilage. , 2003, Biorheology.

[39]  E. Sachlos,et al.  Making tissue engineering scaffolds work. Review: the application of solid freeform fabrication technology to the production of tissue engineering scaffolds. , 2003, European cells & materials.

[40]  Dietmar W. Hutmacher,et al.  Scaffold design and fabrication technologies for engineering tissues — state of the art and future perspectives , 2001, Journal of biomaterials science. Polymer edition.

[41]  P H Krebsbach,et al.  Indirect solid free form fabrication of local and global porous, biomimetic and composite 3D polymer-ceramic scaffolds. , 2003, Biomaterials.

[42]  C. V. van Blitterswijk,et al.  Degradative behaviour of polymeric matrices in (sub)dermal and muscle tissue of the rat: a quantitative study. , 1994, Biomaterials.

[43]  C. Blitterswijk,et al.  The Effect of PEO Ratio on Degradation, Calcification and Bone Bonding of PEO/PBT Copolymer (Polyactive) , 1993 .

[44]  S. Hollister,et al.  Optimal design and fabrication of scaffolds to mimic tissue properties and satisfy biological constraints. , 2002, Biomaterials.