Algae – A quintessential and positive resource of bioethanol production: A comprehensive review

Alternate renewable energy has created a tremendous place for itself in today's scenario of energy studies. Depleting fossil fuels and high environmental pollution leading to threatening climatic changes have led to the search of alternative energy systems. Fuels derived from biological sources, the third generation biofuels – biodiesel, biohydrogen and bioethanol are being explored by researchers and scientists extensively and renewable fuel yielding biomass is now a center of attention. Algae, one such biomass, are unique biological sources with distinctive characters to produce biofuel. They are highly adaptable and can grow in all types of land and water, yielding high content of lipids or carbohydrates. Bioethanol is a biofuel reducing green house gases, carbon-di-oxide emissions and global warming, yet research have been carried out to standardize its potentialities and commercialization. This review throws limelight on potentially utilizing macroalgae for producing bioethanol and application of nanotechnology for extracting carbohydrates for bioethanol production.

[1]  Wu Xiao,et al.  Preparation and characterization of magnetic Fe3O4/CRGO nanocomposites for enzyme immobilization , 2012 .

[2]  P. N. Sarma,et al.  Potential of mixed microalgae to harness biodiesel from ecological water-bodies with simultaneous treatment. , 2011, Bioresource technology.

[3]  Z. Luan,et al.  Montmorillonite–Cu(II)/Fe(III) oxides magnetic material for removal of cyanobacterial Microcystis aeruginosa and its regeneration , 2009 .

[4]  Pedro C. Simões,et al.  Economic analysis of a plant for biodiesel production from waste cooking oil via enzymatic transesterification using supercritical carbon dioxide , 2014 .

[5]  Aikaterini Papazi,et al.  Harvesting Chlorella minutissima using cell coagulants , 2010, Journal of Applied Phycology.

[6]  Hui Wang,et al.  Attached cultivation technology of microalgae for efficient biomass feedstock production. , 2013, Bioresource technology.

[7]  M. Galbe,et al.  A review of the production of ethanol from softwood , 2002, Applied Microbiology and Biotechnology.

[8]  Rosalam Sarbatly,et al.  Conversion of microalgae to biofuel , 2012 .

[9]  Ling Xu,et al.  A simple and rapid harvesting method for microalgae by in situ magnetic separation. , 2011, Bioresource technology.

[10]  Fangwen Bai,et al.  Immobilized lipase of reconstructed oil bodies and its potential application in biodiesel production , 2014 .

[11]  Abdulkadir Baba Hassan,et al.  Salt stress culture of blue-green Algae Spirulina fusiformis , 2003 .

[12]  X. Miao,et al.  Biodiesel production from heterotrophic microalgal oil. , 2006, Bioresource technology.

[13]  Rashmi,et al.  Prospects of biodiesel production from microalgae in India , 2009 .

[14]  Lee R. Lynd,et al.  Conversion of paper sludge to ethanol in a semicontinuous solids-fed reactor , 2003, Bioprocess and biosystems engineering.

[15]  Elba Pinto da Silva Bon,et al.  Evaluation of Chlorella (Chlorophyta) as Source of Fermentable Sugars via Cell Wall Enzymatic Hydrolysis , 2011, Enzyme research.

[16]  J. Pittman,et al.  The potential of sustainable algal biofuel production using wastewater resources. , 2011, Bioresource technology.

[17]  Jelynne P. Tamayo And Ernesto J. Del Rosario Chemical Analysis and Utilization of Sargassum sp. as Substrate for Ethanol Production , 2014 .

[18]  D. Surendhiran,et al.  Microalgal Biodiesel - A Comprehensive Review on the Potential and Alternative Biofuel , 2012 .

[19]  Núria Marbà,et al.  Global unbalance in seaweed production, research effort and biotechnology markets. , 2014, Biotechnology advances.

[20]  Anoop Singh,et al.  Renewable fuels from algae: an answer to debatable land based fuels. , 2011, Bioresource technology.

[21]  Razif Harun,et al.  Exploring alkaline pre-treatment of microalgal biomass for bioethanol production , 2011 .

[22]  Dion Matthew Frederick Frampton,et al.  Production of microalgal concentrates by flocculation and their assessment as aquaculture feeds , 2006 .

[23]  Fathurrahman Lananan,et al.  Harvesting microalgae, Chlorella sp. by bio-flocculation of Moringa oleifera seed derivatives from aquaculture wastewater phytoremediation , 2014 .

[24]  F. Smith,et al.  COLORIMETRIC METHOD FOR DETER-MINATION OF SUGAR AND RELATED SUBSTANCE , 1956 .

[25]  Edgard Gnansounou,et al.  Cyanobacteria and microalgae: a positive prospect for biofuels. , 2011, Bioresource technology.

[26]  W. Liao,et al.  Hydrolysis of animal manure lignocellulosics for reducing sugar production. , 2004, Bioresource technology.

[27]  W. J. Dyer,et al.  A rapid method of total lipid extraction and purification. , 1959, Canadian journal of biochemistry and physiology.

[28]  Aditya M. Kunjapur,et al.  Photobioreactor Design for Commercial Biofuel Production from Microalgae , 2010 .

[29]  Zhiya Ma,et al.  Synthesis of Cibacron Blue F3GA-coupled magnetic PMMA nanospheres and their use for protein affinity separation , 2009 .

[30]  Hu Hong-Ying,et al.  Growth and lipid accumulation properties of a freshwater microalga Scenedesmus sp. under different cultivation temperature. , 2011 .

[31]  Enver Doruk Özdemir,et al.  Land substitution effects of biofuel side products and implications on the land area requirement for EU 2020 biofuel targets , 2009 .

[32]  C. Teixeira,et al.  Evaluation of Moringa oleifera seed flour as a flocculating agent for potential biodiesel producer microalgae , 2011, Journal of Applied Phycology.

[33]  Jo-Shu Chang,et al.  Effect of light intensity and nitrogen starvation on CO2 fixation and lipid/carbohydrate production of an indigenous microalga Scenedesmus obliquus CNW-N. , 2012, Bioresource technology.

[34]  Jie Chang,et al.  Synthesis of biodiesel from waste cooking oil using immobilized lipase in fixed bed reactor. , 2009 .

[35]  J R Ramalingam,et al.  Present scenario of seaweed exploitation and industry in India , 2004 .

[36]  Zhi-Long Ye,et al.  Production and characteristics of a bioflocculant produced by Bacillus sp. F19. , 2008, Bioresource technology.

[37]  Duraiarasan Surendhiran,et al.  Effect of Various Pretreatment for Extracting Intracellular Lipid from Nannochloropsis oculata under Nitrogen Replete and Depleted Conditions , 2014 .

[38]  Adam Harvey,et al.  Assessing the potential of algal biomass opportunities for bioenergy industry: A review , 2015 .

[39]  S. Miyachi,et al.  Wavelength effects on photosynthetic carbon metabolism in Chlorella , 1978 .

[40]  Michael K. Danquah,et al.  Marine microalgae flocculation and focused beam reflectance measurement , 2010 .

[41]  Ram Prasad,et al.  TRIGLYCERIDES-BASED DIESEL FUELS , 2000 .

[42]  A. Kumudha,et al.  Isolation, characterization and outdoor cultivation of green microalgae Botryococcus sp. , 2010 .

[43]  Michael Gross Algal biofuel hopes , 2008, Current Biology.

[44]  Dwi Setyaningsih,et al.  Bioethanol Production from Seaweed Eucheuma cottonii by Neutralization and Detoxification of Acidic Catalyzed Hydrolysate , 2014 .

[45]  M. Ghirardi,et al.  Microalgae: a green source of renewable H(2). , 2000, Trends in biotechnology.

[46]  Peng Wang,et al.  Study on saccharification techniques of seaweed wastes for the transformation of ethanol , 2011 .

[47]  N Kaliaperumal,et al.  Seaweed industry in India , 1999 .

[48]  Masoud Rahimi,et al.  Optimization of biodiesel production from soybean oil in a microreactor , 2014 .

[49]  Ishfaq Ahmad,et al.  A surfactant-coated lipase immobilized in magnetic nanoparticles for multicycle ethyl isovalerate enzymatic production , 2013 .

[50]  Seung Gon Wi,et al.  Bioethanol production from the nutrient stress-induced microalga Chlorella vulgaris by enzymatic hydrolysis and immobilized yeast fermentation. , 2014, Bioresource technology.

[51]  Teresa M. Mata,et al.  Microalgae for biodiesel production and other applications: A review , 2010 .

[52]  B. Dale,et al.  Global potential bioethanol production from wasted crops and crop residues , 2004 .

[53]  Weijie Liu,et al.  A novel bioflocculant produced by a salt-tolerant, alkaliphilic and biofilm-forming strain Bacillus agaradhaerens C9 and its application in harvesting Chlorella minutissima UTEX2341 , 2015 .

[54]  Song Qin,et al.  Transforming kelp into a marine bioreactor. , 2005, Trends in biotechnology.

[55]  Jorge Alberto Vieira Costa,et al.  The role of biochemical engineering in the production of biofuels from microalgae. , 2011, Bioresource technology.

[56]  Thi Hong Minh Nguyen,et al.  Bioethanol production from marine algae biomass: prospect and troubles , 2012 .

[57]  Azmi Telefoncu,et al.  Improving the stability of cellulase by immobilization on modified polyvinyl alcohol coated chitosan beads , 2007 .

[58]  M. Taherzadeh,et al.  Acid-based hydrolysis processes for ethanol from lignocellulosic materials: A review , 2007, BioResources.

[59]  Mika Sillanpää,et al.  Water purification using magnetic assistance: a review. , 2010, Journal of hazardous materials.

[60]  G. Charles Dismukes,et al.  Boosting Autofermentation Rates and Product Yields with Sodium Stress Cycling: Application to Production of Renewable Fuels by Cyanobacteria , 2010, Applied and Environmental Microbiology.

[61]  Duraiarasan Surendhiran,et al.  A green synthesis of antimicrobial compounds from marine microalgae Nannochloropsis oculata , 2014 .

[62]  C. B. Murphy Thermoanalytical Methods of Investigation , 1967 .

[63]  Hongli Zheng,et al.  Harvesting of microalgae by flocculation with poly (γ-glutamic acid). , 2012, Bioresource technology.

[64]  Ronald L. Crawford,et al.  Novel method for immobilization of enzymes to magnetic nanoparticles , 2008 .

[65]  Paul D. Cotter,et al.  Nucleic acid-based approaches to investigate microbial-related cheese quality defects , 2012, Front. Microbio..

[66]  I. Hong,et al.  Comparison of red, brown and green seaweeds on enzymatic saccharification process , 2014 .

[67]  Vaibhav A. Mantri,et al.  Indian seaweed resources and sustainable utilization: Scenario at the dawn of a new century , 2006 .

[68]  Bing Wang,et al.  Direct bioconversion of brown algae into ethanol by thermophilic bacterium Defluviitalea phaphyphila , 2016, Biotechnology for Biofuels.

[69]  Bai-cheng Zhou,et al.  Effect of iron on growth and lipid accumulation in Chlorella vulgaris. , 2008, Bioresource technology.

[70]  Chuanhong Chen,et al.  Bioflocculant produced by Chlamydomonas reinhardtii , 2012, Journal of Applied Phycology.

[71]  G. Mtui,et al.  Bioconversion of lignocellulosic waste from selected dumping sites in Dar es Salaam, Tanzania , 2005, Biodegradation.

[72]  M. Higgins,et al.  Examination of three theories for mechanisms of cation-induced bioflocculation. , 2002, Water research.

[73]  P. Spolaore,et al.  Commercial applications of microalgae. , 2006, Journal of bioscience and bioengineering.

[74]  E. E. Powell,et al.  Economic assessment of an integrated bioethanol–biodiesel–microbial fuel cell facility utilizing yeast and photosynthetic algae , 2009 .

[75]  Shui-Tein Chen,et al.  Catalysis of Rice Straw Hydrolysis by the Combination of Immobilized Cellulase from Aspergillus niger on β-Cyclodextrin-Fe3O4 Nanoparticles and Ionic Liquid , 2015, BioMed research international.

[76]  S. Kawai,et al.  Biofuel Production Based on Carbohydrates from Both Brown and Red Macroalgae: Recent Developments in Key Biotechnologies , 2016, International journal of molecular sciences.

[77]  Seung Gon Wi,et al.  Efficient approach for bioethanol production from red seaweed Gelidium amansii. , 2015, Bioresource technology.

[78]  Sandhya Mishra,et al.  Effects of different media composition, light intensity and photoperiod on morphology and physiology of freshwater microalgae Ankistrodesmus falcatus--a potential strain for bio-fuel production. , 2014, Bioresource technology.

[79]  R. M. Filho,et al.  Lime Pretreatment of Sugarcane Bagasse for Bioethanol Production , 2009, Applied biochemistry and biotechnology.

[80]  Arbakariya B. Ariff,et al.  Effect of different flocculants on the flocculation performance of microalgae, Chaetoceros calcitrans, cells , 2009 .

[81]  Yu-Shen Cheng,et al.  The impact of cell wall carbohydrate composition on the chitosan flocculation of Chlorella , 2011 .

[82]  Shu-wen Huang,et al.  Bioethanol production using carbohydrate-rich microalgae biomass as feedstock. , 2013, Bioresource technology.

[83]  Bo Hu,et al.  Microalgae Harvest through Fungal Pelletization—Co-Culture of Chlorella vulgaris and Aspergillus niger , 2014 .

[84]  Reinu E. Abraham,et al.  Suitability of magnetic nanoparticle immobilised cellulases in enhancing enzymatic saccharification of pretreated hemp biomass , 2014, Biotechnology for Biofuels.

[85]  B. Cheirsilp,et al.  Effect of nitrogen, salt, and iron content in the growth medium and light intensity on lipid production by microalgae isolated from freshwater sources in Thailand. , 2011, Bioresource technology.

[86]  Lei Wang,et al.  Ultrasound assisted extraction of carbohydrates from microalgae as feedstock for yeast fermentation. , 2013, Bioresource technology.

[87]  Katja Loos,et al.  Immobilization of biocatalysts for enzymatic polymerizations: possibilities, advantages, applications. , 2012, Bioresource technology.

[88]  Ryuji Nishiyama,et al.  Identification of a 4-Deoxy-l-erythro-5-hexoseulose Uronic Acid Reductase, FlRed, in an Alginolytic Bacterium Flavobacterium sp. Strain UMI-01 , 2015, Marine drugs.

[89]  Wataru Hashimoto,et al.  Bioethanol production from marine biomass alginate by metabolically engineered bacteria , 2011 .

[90]  Sirajunnisa Abdul Razack,et al.  Medium optimization for the production of exopolysaccharide by Bacillus subtilis using synthetic sources and agro wastes , 2013 .

[91]  Razif Harun,et al.  Microalgal biomass as a fermentation feedstock for bioethanol production , 2009 .

[92]  Giuseppe Torzillo,et al.  Scenedesmus incrassatulus CLHE-Si01: a potential source of renewable lipid for high quality biodiesel production. , 2013, Bioresource technology.

[93]  S. Mayfield,et al.  Exploiting diversity and synthetic biology for the production of algal biofuels , 2012, Nature.

[94]  D. L. Balkwill,et al.  Effects of Iron Starvation on the Physiology of the Cyanobacterium Agmenellum quadruplicatum , 1983, Applied and environmental microbiology.

[95]  Yan Zhang,et al.  Preparation and characterization of magnetic Fe3O4/CRGO nanocomposites for enzyme immobilization , 2012 .

[96]  M. Iqbal,et al.  Effects of photon flux density, CO2, aeration rate, and inoculum density on growth and extracellular polysaccharide production byPorphyridium cruentum , 1993, Folia Microbiologica.

[97]  S. J. Lee,et al.  Design and development of synthetic microbial platform cells for bioenergy , 2013, Front. Microbiol..

[98]  Song Xue,et al.  Identification of carbohydrates as the major carbon sink of the marine microalga Isochrysis zhangjiangensis (Haptophyta) and optimization of its productivity by nitrogen manipulation. , 2014, Bioresource technology.

[99]  Qingfang He,et al.  Assessment of Environmental Stresses for Enhanced Microalgal Biofuel Production – An Overview , 2014, Front. Energy Res..

[100]  René H. Wijffels,et al.  Ultrasound, a new separation technique to harvest microalgae , 2003, Journal of Applied Phycology.

[101]  Abdul Razack Sirajunnisa,et al.  An alternative method for production of microalgal biodiesel using novel Bacillus lipase , 2015, 3 Biotech.

[102]  Duraiarasan Surendhiran,et al.  Influence of bioflocculation parameters on harvesting Chlorella salina and its optimization using response surface methodology , 2013 .

[103]  Se-Kwon Kim,et al.  Statistical optimization of microalgae Pavlova lutheri cultivation conditions and its fermentation conditions by yeast, Candida rugopelliculosa. , 2012, Bioresource technology.

[104]  Jo-Shu Chang,et al.  Enzymatic transesterification of microalgal oil from Chlorella vulgaris ESP-31 for biodiesel synthesis using immobilized Burkholderia lipase. , 2012, Bioresource technology.

[105]  F. Bux,et al.  Dual role of microalgae: Phycoremediation of domestic wastewater and biomass production for sustainable biofuels production , 2011 .

[106]  R. P. John,et al.  Micro and macroalgal biomass: a renewable source for bioethanol. , 2011, Bioresource technology.

[107]  Y. Bashan,et al.  Heterotrophic cultures of microalgae: metabolism and potential products. , 2011, Water research.

[108]  Jack Legrand,et al.  Effects of nutrient deprivation on biochemical compositions and photo-hydrogen production of Tetraselmis subcordiformis , 2011 .

[109]  Wen‐Teng Wu,et al.  Hydrolysis of microalgae cell walls for production of reducing sugar and lipid extraction. , 2010, Bioresource technology.

[110]  P. Bisen,et al.  Biodiesel production with special emphasis on lipase-catalyzed transesterification , 2010, Biotechnology Letters.

[111]  Hyoung‐Chin Kim,et al.  Harvesting of Chlorella vulgaris using a bioflocculant from Paenibacillus sp. AM49 , 2001, Biotechnology Letters.

[112]  António A. Vicente,et al.  Third generation biofuels from microalgae , 2010 .

[113]  Ganti S. Murthy,et al.  Effects of Environmental Factors and Nutrient Availability on the Biochemical Composition of Algae for Biofuels Production: A Review , 2013 .

[114]  Aloys Hüttermann,et al.  Sustainable global energy supply based on lignocellulosic biomass from afforestation of degraded areas , 2009, Naturwissenschaften.

[115]  Mustafa Balat,et al.  Production of bioethanol from lignocellulosic materials via the biochemical pathway: a review. , 2011 .

[116]  Abdul Razack Sirajunnisa,et al.  Biodiesel production from marine microalga Chlorella salina using whole cell yeast immobilized on sugarcane bagasse , 2014 .

[117]  Yong-Ha Park,et al.  Harvest of Scenedesmus sp. with bioflocculant and reuse of culture medium for subsequent high-density cultures. , 2011, Bioresource technology.

[118]  X. Miao,et al.  High yield bio-oil production from fast pyrolysis by metabolic controlling of Chlorella protothecoides. , 2004, Journal of biotechnology.

[119]  S. Sim,et al.  Enzymatic pretreatment of Chlamydomonas reinhardtii biomass for ethanol production. , 2010, Bioresource technology.

[120]  Nei Pereira,et al.  Simultaneous saccharification and fermentation process of different cellulosic substrates using a recombinant Saccharomyces cerevisiae harbouring the β-glucosidase gene , 2010 .

[121]  Y. J. Kim,et al.  Use of Gelidium amansii as a promising resource for bioethanol: a practical approach for continuous dilute-acid hydrolysis and fermentation. , 2012, Bioresource technology.

[122]  Prasant Kumar Rout,et al.  Production of first and second generation biofuels: A comprehensive review , 2010 .

[123]  Y. Chisti Biodiesel from microalgae. , 2007, Biotechnology advances.

[124]  J. Folch,et al.  A simple method for the isolation and purification of total lipides from animal tissues. , 1957, The Journal of biological chemistry.

[125]  Boudewijn Meesschaert,et al.  Flocculation of microalgae using cationic starch , 2009, Journal of Applied Phycology.

[126]  Tianwei Tan,et al.  Pilot-scale production of microbial lipid using starch wastewater as raw material. , 2010, Bioresource technology.

[127]  Duraiarasan Surendhiran,et al.  Exploration on Bioflocculation of Nannochloropsis oculata Using Response Surface Methodology for Biodiesel Production , 2014, TheScientificWorldJournal.

[128]  Archana Tiwari,et al.  Algae as a feedstock for bioethanol production: new entrance in biofuel world. , 2014 .

[129]  Jorge Alberto,et al.  Increment of carbohydrate concentration of Chlorella minutissima microalgae for bioethanol production , 2014 .

[130]  Keat-Teong Lee,et al.  Enzymatic hydrolysis and fermentation of seaweed solid wastes for bioethanol production: An optimization study , 2014 .

[131]  Rishi Gupta,et al.  Bioethanol production from Gracilaria verrucosa, a red alga, in a biorefinery approach. , 2013, Bioresource technology.

[132]  Mohammad J. Taherzadeh,et al.  ENZYMATIC-BASED HYDROLYSIS PROCESSES FOR ETHANOL , 2007 .

[133]  S. Kaul,et al.  Nano‐adsorbents for the removal of metallic pollutants from water and wastewater , 2009, Environmental technology.

[134]  Chiun-Hsun Chen,et al.  Lipid accumulation and CO2 utilization of Nannochloropsis oculata in response to CO2 aeration. , 2009, Bioresource technology.

[135]  Stanley M. Barnett,et al.  Effect of light quality on production of extracellular polysaccharides and growth rate of Porphyridium cruentum , 2004 .

[136]  A. Demain,et al.  Cellulase, Clostridia, and Ethanol , 2005, Microbiology and Molecular Biology Reviews.

[138]  Xianliang Zheng,et al.  A novel and robust recombinant Pichia pastoris yeast whole cell biocatalyst with intracellular overexpression of a Thermomyces lanuginosus lipase: preparation, characterization and application in biodiesel production. , 2014, Bioresource technology.

[139]  Yangyang Jiang,et al.  Magnetic nanoparticles supported ionic liquids for lipase immobilization: Enzyme activity in catalyzing esterification , 2009 .

[140]  F. Bux,et al.  Bioprospecting for hyper-lipid producing microalgal strains for sustainable biofuel production. , 2011, Bioresource technology.

[141]  W. Zhang,et al.  The Isolation and Performance Studies of an Alginate Degrading and Ethanol Producing Strain , 2014 .

[142]  Virendra K Rathod,et al.  Ultrasound assisted enzyme catalyzed transesterification of waste cooking oil with dimethyl carbonate. , 2013, Ultrasonics sonochemistry.

[143]  Zhi‐hua Liu,et al.  Simultaneous saccharification and fermentation of steam-exploded corn stover at high glucan loading and high temperature , 2014, Biotechnology for Biofuels.

[144]  B. Sivaprakash,et al.  Kinetic modeling of microalgal growth and lipid synthesis for biodiesel production , 2014, 3 Biotech.

[145]  Jing Gao,et al.  Pickering emulsion stabilized by lipase-containing periodic mesoporous organosilica particles: a robust biocatalyst system for biodiesel production. , 2014, Bioresource technology.

[146]  Sirajunnisa Abdul Razack,et al.  Medium optimization and in vitro antioxidant activity of exopolysaccharide produced by Bacillus subtilis , 2013, Korean Journal of Chemical Engineering.

[147]  A. Ahmad,et al.  Microalgae as a sustainable energy source for biodiesel production: A review , 2011 .

[148]  I. Ross,et al.  Selection, breeding and engineering of microalgae for bioenergy and biofuel production. , 2012, Trends in biotechnology.

[149]  René H. Wijffels,et al.  Harvesting of microalgae by bio-flocculation , 2010, Journal of Applied Phycology.

[150]  Robert J. Anderson,et al.  South African seaweed aquaculture: A sustainable development example for other African coastal countries , 2013 .

[151]  Madan Lal Verma,et al.  Nanobiotechnology as a novel paradigm for enzyme immobilisation and stabilisation with potential applications in biodiesel production , 2012, Applied Microbiology and Biotechnology.

[152]  Christine Nicole S. Santos,et al.  An Engineered Microbial Platform for Direct Biofuel Production from Brown Macroalgae , 2012, Science.

[153]  Imogen Foubert,et al.  Optimization of an Analytical Procedure for Extraction of Lipids from Microalgae , 2012 .

[154]  I. Chatzipavlidis,et al.  Carbohydrates Production and Bio-flocculation Characteristics in Cultures of Arthrospira (Spirulina) platensis: Improvements Through Phosphorus Limitation Process , 2012, BioEnergy Research.

[155]  C. Posten,et al.  Second Generation Biofuels: High-Efficiency Microalgae for Biodiesel Production , 2008, BioEnergy Research.

[156]  N. Trivedi,et al.  Enzymatic hydrolysis and production of bioethanol from common macrophytic green alga Ulva fasciata Delile. , 2013, Bioresource technology.

[157]  Michimasa Kishimoto,et al.  Effective cell harvesting of the halotolerant microalga Dunaliella tertiolecta with pH control. , 2003, Journal of bioscience and bioengineering.

[158]  Xiaoyan Yuan,et al.  Immobilization of cellulase in nanofibrous PVA membranes by electrospinning , 2005 .

[159]  B. Riaño,et al.  Optimization of chitosan flocculation for microalgal-bacterial biomass harvesting via response surface methodology , 2012 .

[160]  C. Posten,et al.  Microalgae and terrestrial biomass as source for fuels--a process view. , 2009, Journal of biotechnology.

[161]  Gwi-Taek Jeong,et al.  Bioethanol production from the acid hydrolysate of the carrageenophyte Kappaphycus alvarezii (cottonii) , 2011, Journal of Applied Phycology.

[162]  Jufang Wang,et al.  A Comparison of the Production of Ethanol between Simultaneous Saccharification and Fermentation and Separate Hydrolysis and Fermentation Using Unpretreated Cassava Pulp and Enzyme Cocktail , 2012, Bioscience, biotechnology, and biochemistry.

[163]  Yu Cao,et al.  Effect of nitrogen-starvation, light intensity and iron on triacylglyceride/carbohydrate production and fatty acid profile of Neochloris oleoabundans HK-129 by a two-stage process. , 2014, Bioresource technology.

[164]  Zhenhong Yuan,et al.  Ethanol production from high solids loading of alkali-pretreated sugarcane bagasse with an SSF process. , 2014 .

[165]  Mitsuyoshi Ueda,et al.  Molecular Breeding of Advanced Microorganisms for Biofuel Production , 2011, Journal of biomedicine & biotechnology.

[166]  D. Vandamme,et al.  Flocculation of Chlorella vulgaris induced by high pH: role of magnesium and calcium and practical implications. , 2012, Bioresource technology.

[167]  C. Ganesh Kumar,et al.  Characterization of an extracellular biopolymer flocculant from a haloalkalophilic Bacillus isolate , 2004 .

[168]  K. Gao,et al.  Optimization of growth and fatty acid composition of a unicellular marine picoplankton, Nannochloropsis sp., with enriched carbon sources , 2003, Biotechnology Letters.

[169]  Manuel Ferrer,et al.  Environmental biocatalysis: from remediation with enzymes to novel green processes. , 2006, Trends in biotechnology.

[170]  W. Verstraete,et al.  Wet oxidation pre-treatment of woody yard waste: parameter optimization and enzymatic digestibility for ethanol production , 2004 .

[171]  C. Largeau,et al.  Botryococcus braunii: a rich source for hydrocarbons and related ether lipids , 2005, Applied Microbiology and Biotechnology.

[172]  Eun Yeol Lee,et al.  Highly efficient extraction and lipase-catalyzed transesterification of triglycerides from Chlorella sp. KR-1 for production of biodiesel. , 2013, Bioresource technology.

[173]  Milton Sommerfeld,et al.  Photosynthetic carbon partitioning and lipid production in the oleaginous microalga Pseudochlorococcum sp. (Chlorophyceae) under nitrogen-limited conditions. , 2011, Bioresource technology.

[174]  Rao Y. Surampalli,et al.  Biodiesel production from heterotrophic microalgae through transesterification and nanotechnology application in the production , 2013 .

[175]  M. Demirbas,et al.  IMPORTANCE OF ALGAE OIL AS A SOURCE OF BIODIESEL , 2011 .

[176]  Havva Balat,et al.  Progress in biodiesel processing , 2010 .

[177]  Illman,et al.  Increase in Chlorella strains calorific values when grown in low nitrogen medium. , 2000, Enzyme and microbial technology.

[178]  Jane-Yii Wu,et al.  Sequential acid and enzymatic hydrolysis in situ and bioethanol production from Gracilaria biomass. , 2014, Bioresource technology.

[179]  Zsófia Kádár,et al.  Simultaneous saccharification and fermentation (SSF) of industrial wastes for the production of ethanol , 2004 .

[180]  F. P. Healey,et al.  Indicators of Phosphorus and Nitrogen Deficiency in Five Algae in Culture , 1979 .

[181]  Dongzhi Wei,et al.  Lipase-catalyzed irreversible transesterification of vegetable oils for fatty acid methyl esters production with dimethyl carbonate as the acyl acceptor , 2007 .

[182]  N. De Pauw,et al.  Potential of electrolytic flocculation for recovery of micro-algae , 1997 .

[183]  R. Rengasamy,et al.  Mass culture of Botryococcus braunii Kutz. under open raceway pond for biofuel production. , 2012, Bioresource technology.

[184]  Tao Bai,et al.  Characterization and flocculating properties of a novel bioflocculant produced by Bacillus circulans , 2009 .

[185]  Susan E. Powers,et al.  Sustainable Algae Biodiesel Production in Cold Climates , 2010 .

[186]  Kemka H. Ogbonda,et al.  Influence of temperature and pH on biomass production and protein biosynthesis in a putative Spirulina sp. , 2007, Bioresource technology.

[187]  Ledwoch Katarzyna,et al.  Non-enclosure methods for non-suspended microalgae cultivation: literature review and research needs , 2015 .

[188]  Peter McKendry,et al.  Energy production from biomass (Part 2): Conversion technologies. , 2002, Bioresource technology.

[189]  Philip Owende,et al.  Biofuels from microalgae—A review of technologies for production, processing, and extractions of biofuels and co-products , 2010 .

[190]  Feng Wang,et al.  pH-Sensitive Magnetic Ion Exchanger for Protein Separation , 2009 .

[191]  Bo Zhang,et al.  Alkali Pretreatment and Enzymatic Hydrolysis of Cattails from Constructed Wetlands , 2010 .

[192]  J. J. Liu,et al.  Industrial-scale bioethanol production from brown algae: Effects of pretreatment processes on plant economics , 2015 .

[193]  Céline Rebours,et al.  Seaweeds: an opportunity for wealth and sustainable livelihood for coastal communities , 2014, Journal of Applied Phycology.

[194]  Yuan-Kun Lee,et al.  Secondary carotenoids formation by the green alga Chlorococcum sp. , 2000, Journal of Applied Phycology.

[195]  S D Varfolomeev,et al.  Production of biofuels from pretreated microalgae biomass by anaerobic fermentation with immobilized Clostridium acetobutylicum cells. , 2012, Bioresource technology.