Design of a biological cell fusion device

A cell fusion device is designed and fabricated in order to electrofuse two cells between electrodes. Dielectrophoretic force is used to attract each cell and make a pearl chain of two cells. The cells are fed from two anisotropically etched cavities of a silicon substrate. This method is helpful to integrate other devices: micro pump, valves and selectors. Also two kinds of the electrode array are fabricated and tested the: feasibility of the proposed device. The attraction of two radish cells on the electrodes of the electrode array is observed when AC voltage(1 MHz, V/sub p-p/ =8V) is applied to the electrodes.

[1]  M. Washizu.  Manipulation of biological objects in micromachined structures , 1992, [1992] Proceedings IEEE Micro Electro Mechanical Systems.

[2]  E. Neumann,et al.  Electroporation and Electrofusion in Cell Biology , 1989, Springer US.

[3]  Yong-Kweon Kim,et al.  Development of cell-grabber by using piezo actuator , 1994, Proceedings of 16th Annual International Conference of the IEEE Engineering in Medicine and Biology Society.

[4]  P. Bergveld,et al.  Polymer bonding of micro-machined silicon structures , 1992, [1992] Proceedings IEEE Micro Electro Mechanical Systems.

[5]  S. D. Collins,et al.  Micromachined packaging for chemical microsensors , 1988 .

[6]  Mark G. Allen,et al.  A planar variable reluctance magnetic micromotor with fully integrated stator and coils , 1993 .

[7]  Kazuo Sato,et al.  Individual and mass operation of biological cells using micromechanical silicon devices , 1990 .

[8]  S. Masuda,et al.  Novel method of cell fusion in field constriction area in fluid integration circuit , 1989 .