Quantification of acetaldehyde released by lung cancer cells in vitro using selected ion flow tube mass spectrometry.

The production of volatile compounds from cancer cell lines in vitro has been investigated using selected ion flow tube mass spectrometry (SIFT-MS). This technique enables on-line quantitative analyses of the headspace above cell/medium cultures. This paper reports the discovery that acetaldehyde is released by the lung cancer cell lines SK-MES and CALU-1. The concentration of acetaldehyde in the headspace of the medium/cell culture was measured after 16 h incubation at 37 degrees C and found to be proportional to the number of cancer cells in the medium (typically 10(8)). From these data, the acetaldehyde production rates of the SK-MES cells and the CALU-1 cells in vitro are determined to be 1 x 10(6) and 1.5-3 x 10(6) molecules/cell/min, respectively. The potential value of this new technique in cell biology and in industrial cell biotechnology is discussed.

[1]  N. Homann.  Alcohol and upper gastrointestinal tract cancer: the role of local acetaldehyde production , 2001, Addiction biology.

[2]  M. Phillips,et al.  Ion-trap detection of volatile organic compounds in alveolar breath. , 1992, Clinical chemistry.

[3]  D. Taatjes,et al.  Mass spectrometric measurement of formaldehyde generated in breast cancer cells upon treatment with anthracycline antitumor drugs. , 2000, Chemical research in toxicology.

[4]  D. Tuma,et al.  Inhibition of glycoprotein secretion by ethanol and acetaldehyde in rat liver slices. , 1980, Biochemical pharmacology.

[5]  P. Sobotka,et al.  Expired hydrocarbons in patients with acute myocardial infarction. , 1995, Free radical research.

[6]  P. Španěl,et al.  The novel selected-ion flow tube approach to trace gas analysis of air and breath. , 1996, Rapid communications in mass spectrometry : RCM.

[7]  Ari J. S. Ferreira,et al.  Elucidation of the Metabolic Fate of Glucose in the Filamentous Fungus Trichoderma reesei Using Expressed Sequence Tag (EST) Analysis and cDNA Microarrays* , 2002, The Journal of Biological Chemistry.

[8]  V. Bierbaum,et al.  Formaldehyde in human cancer cells: detection by preconcentration-chemical ionization mass spectrometry. , 2001, Analytical chemistry.

[9]  A. Manolis,et al.  The diagnostic potential of breath analysis. , 1983, Clinical chemistry.

[10]  A. Hołownia,et al.  Acetaldehyde cytotoxicity in cultured rat astrocytes , 1999, Brain Research.

[11]  Tianshu Wang,et al.  Quantification of volatile compounds in the headspace of aqueous liquids using selected ion flow tube mass spectrometry. , 2002, Rapid communications in mass spectrometry : RCM.

[12]  P. Španěl,et al.  A new 'online' method to measure increased exhaled isoprene in end-stage renal failure. , 2001, Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association.

[13]  Tianshu Wang,et al.  Kinetics and isotope patterns of ethanol and acetaldehyde emissions from yeast fermentations of glucose and glucose-6,6-d2 using selected ion flow tube mass spectrometry: a case study. , 2002, Rapid communications in mass spectrometry : RCM.

[14]  D. Tuma,et al.  Relationship between acetaldehyde levels and cell survival in ethanol‐metabolizing hepatoma cells , 2002, Hepatology.

[15]  Taylor Murray,et al.  Cancer statistics, 1999 , 1999, CA: a cancer journal for clinicians.

[16]  David Smith,et al.  Time variation of ammonia, acetone, isoprene and ethanol in breath: a quantitative SIFT-MS study over 30 days. , 2003, Physiological measurement.

[17]  A. Jones.  Measuring and reporting the concentration of acetaldehyde in human breath. , 1995, Alcohol and alcoholism.

[18]  David Smith,et al.  On-line, simultaneous quantification of ethanol, some metabolites and water vapour in breath following the ingestion of alcohol. , 2002, Physiological measurement.

[19]  P. Mortensen,et al.  Kinetic studies on colonocyte metabolism of short chain fatty acids and glucose in ulcerative colitis. , 1995, Gut.

[20]  H. J. O’neill,et al.  A computerized classification technique for screening for the presence of breath biomarkers in lung cancer. , 1988, Clinical chemistry.

[21]  P. Španěl,et al.  Quantitative analysis of ammonia on the breath of patients in end-stage renal failure. , 1997, Kidney international.

[22]  Patrik Španěl,et al.  Application of ion chemistry and the SIFT technique to the quantitative analysis of trace gases in air and on breath , 1996 .

[23]  P. Španěl,et al.  Trace gases in breath of healthy volunteers when fasting and after a protein-calorie meal: a preliminary study. , 1999, Journal of applied physiology.

[24]  T. Holland,et al.  Analysis of formaldehyde in the headspace of urine from bladder and prostate cancer patients using selected ion flow tube mass spectrometry. , 1999, Rapid communications in mass spectrometry : RCM.

[25]  T. Adrian,et al.  A factor from pancreatic and colonic cancer cells stimulates glucose uptake and lactate production in myoblasts. , 1999, Biochemical and biophysical research communications.

[26]  G. Crawford,et al.  Mechanisms of acetaldehyde-mediated growth inhibition: delayed cell cycle progression and induction of apoptosis. , 1995, Alcoholism, clinical and experimental research.

[27]  D. Danks,et al.  Profiles of urinary volatiles from metabolic disorders characterized by unusual odors. , 1983, Clinical chemistry.

[28]  G. A. Dawson,et al.  Tropospheric light alcohols, carbonyls, and acetonitrile: Concentrations in the southwestern United States and Henry's Law data , 1985 .

[29]  T. Risby,et al.  Breath isoprene: temporal changes in respiratory output after exposure to ozone. , 1996, Journal of applied physiology.

[30]  T. Orfeo,et al.  One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. , 1977, Journal of the National Cancer Institute.