The RuvABC proteins and Holliday junction processing in Escherichia coli

Until five years ago, little was known about the way in which bacteria processed recombination intermediates into mature recombinant products. Genetic studies of recombination, in combination with detailed biochemical studies of the Escherichia coli recombination protein RecA indicated that recombining chromosomes (and plasmids) were linked by a crossover, or Holliday junction (Fig. 1), and it was reasonable to assume that these junctions served as a substrate for a specific junction-resolving endonuclease. Unfortunately, biochemical attempts to detect this endonuclease had been unsuccessful. Similarly, genetic screens had failed to identify a mutant that exhibited a clear resolution-defective phenotype. The prospects were not looking good.

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