Delivery of picosecond lasers in multimode fibers for coherent anti-Stokes Raman scattering imaging.

We investigated the possibility of using standard commercial multimode fibers (MMF), Corning SMF28 fibers, to deliver picosecond excitation lasers for coherent anti-Stokes Raman scattering (CARS) imaging. We theoretically and/or experimentally analyzed issues associated with the fiber delivery, such as dispersion length, walk-off length, nonlinear length, average threshold power for self-phase modulations, and four-wave mixing (FWM). These analyses can also be applied to other types of fibers. We found that FWM signals are generated in MMF, but they can be filtered out using a long-pass filter for CARS imaging. Finally, we demonstrated that MMF can be used for delivery of picosecond excitation lasers in the CARS imaging system without any degradation of image quality.

[1]  X. Xie,et al.  Vibrational imaging of lipid droplets in live fibroblast cells with coherent anti-Stokes Raman scattering microscopy Published, JLR Papers in Press, August 16, 2003. DOI 10.1194/jlr.D300022-JLR200 , 2003, Journal of Lipid Research.

[2]  Robert R. Alfano,et al.  Intensity effects on the stimulated four photon spectra generated by picosecond pulses in optical fibers , 1987 .

[3]  Siddharth Ramachandran,et al.  Analysis of optical response of long period fiber gratings to nm-thick thin-film coating. , 2005, Optics express.

[4]  Ji-Xin Cheng,et al.  Coherent Anti-Stokes Raman Scattering Microscopy , 2007, 2008 Conference on Lasers and Electro-Optics and 2008 Conference on Quantum Electronics and Laser Science.

[5]  Andreas Zumbusch,et al.  Coherent anti-Stokes Raman scattering microscopy , 1999 .

[6]  X. Xie,et al.  Coherent Anti-Stokes Raman Scattering Microscopy: Instrumentation, Theory, and Applications , 2004 .

[7]  R. Stolen Phase-matched-stimulated four-photon mixing in silica-fiber waveguides , 1975 .

[8]  J. Fujimoto,et al.  In vivo endoscopic optical biopsy with optical coherence tomography. , 1997, Science.

[9]  Gangjun Liu,et al.  Fiber delivered probe for efficient CARS imaging of tissues. , 2010, Optics express.

[10]  Andreas Volkmer,et al.  Multiplex Coherent Anti-Stokes Raman Scattering Microspectroscopy and Study of Lipid Vesicles , 2002 .

[11]  E. Vicaut,et al.  Fibered Confocal Fluorescence Microscopy (Cell-viZio™) Facilitates Extended Imaging in the Field of Microcirculation , 2004, Journal of Vascular Research.

[12]  Masaharu Ohashi,et al.  Four-wave mixing suppression effect of dispersion distributed fibers , 1999 .

[13]  Jane A Dickerson,et al.  Coherent anti-stokes Raman scattering microscopy: chemical imaging for biology and medicine. , 2008, Annual review of analytical chemistry.

[14]  E. Cocker,et al.  Fiber-optic fluorescence imaging , 2005, Nature Methods.

[15]  X. Xie,et al.  Towards CARS Endoscopy. , 2006, Optics express.

[16]  Jin U. Kang,et al.  All-fiber common-path optical coherence tomography: sensitivity optimization and system analysis , 2005, IEEE Journal of Selected Topics in Quantum Electronics.

[17]  Feruz Ganikhanov,et al.  High-sensitivity vibrational imaging with frequency modulation coherent anti-Stokes Raman scattering (FM CARS) microscopy. , 2006, Optics letters.

[18]  Ji-Xin Cheng,et al.  Coherent anti-Stokes Raman scattering imaging with a laser source delivered by a photonic crystal fiber. , 2006, Optics letters.

[19]  Juleon M. Schins,et al.  Imaging the Thermodynamic State of Lipid Membranes with Multiplex CARS Microscopy , 2002 .

[20]  Alistair Elfick,et al.  A versatile CARS microscope for biological imaging , 2009 .

[21]  Conor L Evans,et al.  Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[22]  Stephen T. C. Wong,et al.  Chemically-selective imaging of brain structures with CARS microscopy. , 2007, Optics express.

[23]  K. Inoue Four-wave mixing in an optical fiber in the zero-dispersion wavelength region , 1992 .