Kinetic model for the simulation of hen egg white lysozyme adsorption at solid/water interface

A simulation model for adsorption kinetics of hen egg white lysozyme (HEW) adsorption to hydrophilic silica is proposed. The adsorption kinetic data were monitored by usingin-situ ellipsometry. The model is based on an irreversible adsorption mechanism allowing two different adsorbed states. The adsorbed states were differentiated based on binding strengths resistant to the concentration gradient exerted by rinse. Molecules desorbing and remaining upon rinse were identified as loosely bound (state 1) and tightly bound (state 2) states, respectively. The adsorption rate constants were assumed to be a time-dependent nonlinear function in order to account for the change in surface properties originating from the protein layer formed on the surface. The parameters of adsorption rate constants were evaluated by using adsorption kinetic data at different protein concentrations, and the relationships between the adsorption parameters and protein concentration were established which eventually demonstrated a linear relationship. The established relations between the adsorption parameters and concentration elucidated the effect of protein concentration on adsorption to hydrophilic silica.

[1]  B. Matthews,et al.  Letter: crystallographic data fro lysoxyme from bacteriophage T4. , 1973, Journal of molecular biology.

[2]  J. Janson.  Optimization of large-scale chromatography of proteins , 2001 .

[3]  A. Gast,et al.  Manipulation of hydrophobic interactions in protein adsorption , 1991 .

[4]  Adamczyk.  Kinetics of Diffusion-Controlled Adsorption of Colloid Particles and Proteins. , 2000, Journal of colloid and interface science.

[5]  M. Wahlgren,et al.  Structural Stability Effects on the Adsorption and Dodecyltrimethylammonium Bromide-Mediated Elutability of Bacteriophage T4 Lysozyme at Silica Surfaces , 1995 .

[6]  Adsorption of globular model proteins to silica and methylated silica surfaces and their elutability by dodecyltrimethylammonium bromide , 1993 .

[7]  E. Leonard,et al.  Sorption kinetics of binary protein solutions: General approach to multicomponent systems , 1982 .

[8]  Stuart L. Cooper,et al.  Protein adsorption on polymeric biomaterials I. Adsorption isotherms , 1988 .

[9]  B. Matthews,et al.  Structure of bacteriophage T4 lysozyme refined at 1.7 A resolution. , 1987, Journal of molecular biology.

[10]  J. Andrade.  Surface and Interfacial Aspects of Biomedical Polymers , 1985 .

[11]  Arunan Nadarajah,et al.  A Comprehensive Model of Multiprotein Adsorption on Surfaces , 1994 .

[12]  S. Cooper,et al.  Protein adsorption on polymeric biomaterials: II. Adsorption kinetics , 1988 .

[13]  W. Norde,et al.  The adsorption of human plasma albumin and bovine pancreas ribonuclease at negatively charged polystyrene surfaces: I. Adsorption isotherms. Effects of charge, ionic strength, and temperature , 1978 .

[14]  M. Malmsten.  Ellipsometry studies of the effects of surface hydrophobicity on protein adsorption , 1995 .

[15]  M. Daeschel,et al.  Interfacial tension kinetics of nisin and β-Casein at an oil-water interface , 2000 .

[16]  A. Kondo,et al.  Structural changes in protein molecules adsorbed on ultrafine silica particles , 1991 .

[17]  K E Healy,et al.  The role of vitronectin in the attachment and spatial distribution of bone-derived cells on materials with patterned surface chemistry. , 1997, Journal of biomedical materials research.

[18]  J. Ko,et al.  Effect of electrostatic interaction on the adsorption of globular proteins on octacalcium phosphate crystal film. , 2002, Journal of colloid and interface science.

[19]  M. Bothwell,et al.  Structural Stability Effects on Adsorption of Bacteriophage T4 Lysozyme to Colloidal Silica , 1998 .

[20]  J. Séquaris,et al.  Principles of Protein , 1980 .

[21]  T. Groth,et al.  Reorganization of substratum-bound fibronectin on hydrophilic and hydrophobic materials is related to biocompatibility , 1994 .

[22]  I. Lundström,et al.  The Adsorption of Lysozyme to Hydrophilic Silicon Oxide Surfaces: Comparison between Experimental Data and Models for Adsorption Kinetics , 1995 .

[23]  Ingemar Lundström,et al.  A wettability gradient method for studies of macromolecular interactions at the liquid/solid interface , 1987 .

[24]  T. Horbett.  Adsorption of proteins from plasma to a series of hydrophilic-hydrophobic copolymers. II. Compositional analysis with the prelabeled protein technique. , 1981, Journal of biomedical materials research.

[25]  A Mechanistic Approach to Modeling Single Protein Adsorption at Solid-Water Interfaces. , 1999, Journal of colloid and interface science.

[26]  W. Norde,et al.  The behavior of some model proteins at solid-liquid interfaces 1. Adsorption from single protein solutions , 1990 .

[27]  H C Hemker,et al.  The adsorption of prothrombin to phosphatidylserine multilayers quantitated by ellipsometry. , 1983, The Journal of biological chemistry.