Tropic Virus Prediction Is Associated with a Nadir CD 4 T-Cell Count below 100 Cells / mm 3

Objective: The aim of this study was to evaluate tropism prediction performances of three algorithms [geno2pheno false-positive rate 10% (G2P10), position-specific scoring matrix (PSSM) and a combination of the 11/25 and net charge rules] and to investigate the viral and host factors potentially involved in the X4 or R5 prediction in human immunodeficiency virus-1 (HIV-1) patients. Methods: Viral tropism was determined in 179 HIV-1-infected patients eligible for CCR5 antagonist therapy. HIV-1 RNA or DNA was extracted and amplified for env gp120 sequencing. In parallel, demographic, viral, immunological and clinical determinants were analyzed. Results: According to the G2P10 algorithm, 48 patients harbored X4 or X4R5 virus. The tropism prediction was concordant for 87.7 and 88.2% of samples when comparing G2P10 with PSSM or with a combination of the 11/25 and net Received: September 10, 2014 Accepted after revision: April 9, 2015 Published online: May 21, 2015 Sandrine Castelain Laboratoire de Virologie, Centre de Biologie Humaine CHU Hôpital Sud FR–80054 Amiens Cedex (France) E-Mail sandrine.castelain @ u-picardie.fr © 2015 S. Karger AG, Basel 0300–5526/15/0583–0155$39.50/0