Genetic relationships among turnip (Brassica rapa var. rapa) genotypes.

Turnip (Brassica rapa var. rapa) is one of the main vegetables consumed by people living in Eastern Anatolia in Turkey. In this region, farmers obtain their own seeds for production, which results in considerable morphological variability. We examined the genetic variation and relationships among 11 turnip genotypes sampled from diverse environments of the Erzurum region located in Eastern Anatolia in Turkey. Thirty-two Operon RAPD primers were screened; among them, 20 gave reproducible and clear DNA fragments after amplification. The average polymorphism ratio was 90.4%. The genetic distance between turnip genotypes were found to range from 0.302 to 0.733, indicating high genetic variability. Eleven genotypes were divided into three main clusters in a dendrogram; ETS2 and ETS8 genotypes were the most distant. We conclude that RAPD analysis would be useful for genotyping turnip genotypes.

[1]  S. Ercişli,et al.  RAPD-based study of genetic variation and relationships among wild fig genotypes in Turkey. , 2009, Genetics and molecular research : GMR.

[2]  S. Ercişli,et al.  Molecular Characterization of Plum Cultivars by AFLP Markers , 2009 .

[3]  S. Ercişli,et al.  Interspecific variability of RAPD and fatty acid composition of some pomegranate cultivars (Punica granatum L.) growing in Southern Anatolia Region in Turkey , 2007 .

[4]  T. Kuang,et al.  A rapid and efficient DNA minipreparation suitable for screening transgenic plants , 2001, Plant Molecular Biology Reporter.

[5]  A. Ordás,et al.  Genetic diversity in a germplasm collection of Brassica rapa subsp rapa L. from northwestern Spain , 2005, Euphytica.

[6]  Muhammad Zubair,et al.  Effect of seedling age and water depth on morphological and physiological aspects of transplanted rice under high temperature. , 2005, Journal of Zhejiang University. Science. B.

[7]  ZubairMuhammad,et al.  Effect of seedling age and water depth on morphological and physiological aspects of transplanted rice under high temperature , 2005 .

[8]  S. Ercişli,et al.  Genetic relationships among grapevine cultivars native to Croatia, Greece and Turkey , 2005 .

[9]  J. Reif,et al.  Wheat genetic diversity trends during domestication and breeding , 2005, Theoretical and Applied Genetics.

[10]  S. Ercişli A short review of the fruit germplasm resources of Turkey , 2004, Genetic Resources and Crop Evolution.

[11]  Tohru Suzuki,et al.  Genetic diversity in mustard (Brassica juncea L.) germplasm from Pakistan as determined by RAPDs , 1998, Euphytica.

[12]  M. Badenes,et al.  Analysis of apricot germplasm from the European ecogeographical group , 1998, Euphytica.

[13]  C. Halldén,et al.  Evaluation of RFLP and RAPD markers in a comparison of Brassica napus breeding lines , 1994, Theoretical and Applied Genetics.

[14]  J. Nienhuis,et al.  Comparison of RAPD and RFLP genetic markers in determining genetic similarity among Brassica oleracea L. genotypes , 1994, Theoretical and Applied Genetics.

[15]  J. Williams,et al.  Characterization of genetic identities and relationships of Brassica oleracea L. via a random amplified polymorphic DNA assay , 1992, Theoretical and Applied Genetics.

[16]  B. Fristensky,et al.  Discrimination among cultivars of rapeseed (Brassica napus L.) using DNA polymorphisms amplified from arbitrary primers , 2004, Theoretical and Applied Genetics.

[17]  A. Ordás,et al.  Relationships Among Brassica napus (L.) Germplasm from Spain and Great Britain as Determined by RAPD Markers , 2005, Genetic Resources and Crop Evolution.

[18]  W. Friedt,et al.  Molecular characterization of novel resynthesized rapeseed (Brassica napus) lines and analysis of their genetic diversity in comparison with spring rapeseed cultivars , 2003 .

[19]  A. Schneider,et al.  Genetic relationships among grapevine cultivars from North-Western Italy , 2003 .

[20]  W. Köhler,et al.  Population Genetics: Aspects of Biodiversity , 2003 .

[21]  R. Cansian,et al.  Discrimination among cultivars of cabbage using randomly amplified polymorphic DNA markers. , 2000 .

[22]  M. Micheli,et al.  Random Amplified Polymorphic DNA Assay , 1997 .

[23]  S. Kresovich,et al.  Identities and Relationships among Chinese Vegetable Brassicas as Determined by Random Amplified Polymorphic DNA Markers , 1995 .

[24]  P. Tanhuanpää,et al.  Genetic polymorphism at RAPD loci in spring turnip rape (Brassica rapa ssp. oleifera) , 1993 .

[25]  L. Gottlieb,et al.  Genetics and Morphological Evolution in Plants , 1984, The American Naturalist.