Original Article Correlation of HSP70, P53 and Bmi-1 in hepatitis, cirrhosis and hepatocellular carcinoma

Objective: To investigate the correlation of HSP70, P53 and Bmi-1 in hepatitis, cirrhosis and hepatocellular carcinoma patients. Methods: Hepatitis (30 cases), cirrhosis (30 cases), early stage HCC (30 cases), and advanced stage HCC (80 cases) patients were recruited in this study, and we used immunohistochemistry S-P method to de- tect the protein expression levels of HSP70, P53 and Bmi-1, which were then correlated with clinical pathological parameters. Results: (1) HSP70, P53 and Bmi 1-expression levels were increased in early stage HCC and advanced HCC, compared to the hepatitis and cirrhosis patients. The result was statistically significant (P < 0.05); and P53 expression in advanced HCC patients was significantly higher than early stage HCC. (2) HSP70 expression level was positively correlated with P53 and Bmi-1 in advanced HCC, (r 1 = 0.45, r 2 = 0.57). P53 expression level was positively correlated with Bmi-1 in advanced HCC (r 3 = 0.45); (3) In advanced HCC patients, HSP70 and P53 expression is correlated with tumor differentiation grade (P < 0.5), regardless of the patient gender, age, tumor size, thrombus status; Bmi-1 expression level correlates with the TNM stage (P < 0.5), regardless of the patient gender, age, tumor size, thrombosis status or tumor differentiation grade. (4) Patients with positive P53, HSP70 and Bmi-1 would have poor prognosis, but the difference was not statistically significant. Conclusion: HSP70, P53 and Bmi-1 are expressed at higher levels in HCC patients. They are probably involved in the development and progression of HCC, and they are playing a synergestic role in the cell proliferation in HCC.