Effect of Ca antagonists on PGI2 generation in cultured human vascular endothelial cells

Endothelial cells were isolated from human umbilical cord and cultured by the modified method of Jaff e et al. 6-keto PGF1a content in supernatant over the monolayer cells was employed as the parameter of PGI2 generation. PGI2 generation was increased by addition of Nif edipine (x 2. 10) >Diltiazem (x 1. 78) Verapamil (x 1. 59). This effect was diminished when the cells were pretreated with each of Mepacrine or ASA, even when Arachidonic acid or PGH2 was added. The increase of PGI2 generation by Ca antagonist was considered to be due to activation of phospholipase A2. The PGI2 generation induced by A-23187, Arachidonic acid, or PGH2 was remarkably decreased by preincubating the cells with TMB-8. The dependency of PGI2 generation upon the intracellular Ca++ was considered to be dominant in the early step of AA cascade. The enhanced PGI2 generation by A-23187 or Diltiazem was almost diminished in Ca++ free medium. While preincubation with MIX caused increase in intracellular c-AMP concentration, and decrease in PGI2 generation, addition of Diltiazem had no

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