Optimization of the dacarbazine liposones preparation with Box-Behnken central composite design

To optimize the DTIC liposome preparation process and study the shape and the properties,we have the experiments according to Box-Behnken central composite principle,with indexes of the encapsulation efficiency,appearance,in vitro release.The Results show that Liposome encapsulation rate is 94.20% which is 0.02% higher than the predicted and the particle size is in 20~50 μm.We come to a conclusion that there are both the burst release and sustained-release in the Liposome,the predicted process conditions is similar to that of the real.The Box-Behnken central composite design can be applied to DTIC liposomes preparation.