Two-step chromatographic purification of glutathione S-transferase-tagged human papillomavirus type 16 E6 protein and its application for serology.

[1]  R. Chiaraluce,et al.  Production of functional, stable, unmutated recombinant human papillomavirus E6 oncoprotein: implications for HPV-tumor diagnosis and therapy , 2016, Journal of Translational Medicine.

[2]  W. Ju,et al.  A lectin-based diagnostic system using circulating antibodies to detect cervical intraepithelial neoplasia and cervical cancer. , 2015, Glycobiology.

[3]  S. Franceschi,et al.  Antibodies against high‐risk human papillomavirus proteins as markers for invasive cervical cancer , 2014, International journal of cancer.

[4]  R. Verma,et al.  E6 protein of human papillomavirus 16 (HPV16) expressed in Escherichia coli sans a stretch of hydrophobic amino acids, enables purification of GST-ΔE6 in the soluble form and retains the binding ability to p53. , 2013, Protein expression and purification.

[5]  Goo-Young Kim,et al.  Improved recovery of active GST-fusion proteins from insoluble aggregates: solubilization and purification conditions using PKM2 and HtrA2 as model proteins. , 2011, BMB reports.

[6]  Cary A Moody,et al.  Human papillomavirus oncoproteins: pathways to transformation , 2010, Nature Reviews Cancer.

[7]  P. Lambert,et al.  Human papillomavirus 16 E6 variants differ in their dysregulation of human keratinocyte differentiation and apoptosis. , 2009, Virology.

[8]  S. Franceschi,et al.  Human papillomavirus type distribution in invasive cervical cancer and high‐grade cervical lesions: A meta‐analysis update , 2007, International journal of cancer.

[9]  C. Deber,et al.  Optimizing synthesis and expression of transmembrane peptides and proteins. , 2007, Methods.

[10]  M. Garcia-Alai,et al.  High-risk HPV E6 oncoproteins assemble into large oligomers that allow localization of endogenous species in prototypic HPV-transformed cell lines. , 2007, Biochemistry.

[11]  C. Meijer,et al.  HPV‐mediated cervical carcinogenesis: concepts and clinical implications , 2006, The Journal of pathology.

[12]  E. Yim,et al.  The role of HPV E6 and E7 oncoproteins in HPV-associated cervical carcinogenesis. , 2005, Cancer research and treatment : official journal of Korean Cancer Association.

[13]  M. Stefani.  Protein misfolding and aggregation: new examples in medicine and biology of the dark side of the protein world. , 2004, Biochimica et biophysica acta.

[14]  H. Bernard,et al.  Chelating agents stabilize the monomeric state of the zinc binding human papillomavirus 16 E6 oncoprotein. , 2003, Biochemistry.

[15]  K. Cooper,et al.  Biotinyl-Tyramide-Based In Situ Hybridization Signal Patterns Distinguish Human Papillomavirus Type and Grade of Cervical Intraepithelial Neoplasia , 2002, Modern Pathology.

[16]  J. Lefèvre,et al.  Formation of soluble inclusion bodies by hpv e6 oncoprotein fused to maltose-binding protein. , 2001, Protein expression and purification.

[17]  G. Amidon,et al.  Protein denaturation during freezing and thawing in phosphate buffer systems: monomeric and tetrameric beta-galactosidase. , 2000, Archives of biochemistry and biophysics.

[18]  D. Wazer,et al.  E7 protein of human papilloma virus-16 induces degradation of retinoblastoma protein through the ubiquitin-proteasome pathway. , 1996, Cancer research.

[19]  C. A. Janeway.  Immunobiology: The Immune System in Health and Disease , 1996 .

[20]  R. Burk,et al.  Persistent genital human papillomavirus infection as a risk factor for persistent cervical dysplasia. , 1995, Journal of the National Cancer Institute.

[21]  V. Moreno,et al.  Prevalence of Human Papillomavirus in Cervical Cancer: a Worldwide Perspective , 1995 .

[22]  G. Demers,et al.  The ability of human papillomavirus E6 proteins to target p53 for degradation in vivo correlates with their ability to abrogate actinomycin D-induced growth arrest , 1994, Journal of virology.

[23]  D. Lowy,et al.  HPV16 E6 and E7 proteins cooperate to immortalize human foreskin keratinocytes. , 1989, The EMBO journal.

[24]  T. Sugimura,et al.  Purification and DNA-binding properties of human papillomavirus type 16 E6 protein expressed in Escherichia coli. , 1989, Biochemical and biophysical research communications.

[25]  R. Schlegel,et al.  The E6 and E7 genes of the human papillomavirus type 16 together are necessary and sufficient for transformation of primary human keratinocytes , 1989, Journal of virology.

[26]  T. Yokotsuka,et al.  Some properties of acid protease from the thermophilic fungus, Penicillium duponti K1014. , 1973, Applied microbiology.

[27]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[28]  K. Yasunobu,et al.  BACILLUS SUBTILIS NEUTRAL PROTEINASE. I. A ZINC ENZYME OF HIGH SPECIFIC ACTIVITY. , 1964, The Journal of biological chemistry.

[29]  D. Speicher,et al.  Purification of proteins fused to glutathione S-transferase. , 2011, Methods in molecular biology.

[30]  Magnus von Knebel Doeberitz,et al.  Type-dependent integration frequency of human papillomavirus genomes in cervical lesions. , 2008, Cancer research.