Expression of the RT6 mono(ADP-ribosyl)transferases is regulated by two promoter regions.

The structure of the RT6 mono(ADP-ribosyl)transferase gene was studied. Analysis of cDNA clones revealed eight exons and suggested two independent transcriptional start sites. The existence of the downstream initiation site was confirmed by S1-nuclease protection and localized to position +29 of exon 2. The corresponding 5' flanking regions were found to contain typical promoter structures such as TATA- and CCAAT-boxes. Comparison with sequences deposited in the TRANSFAC database of transcription factor binding sites revealed few putative regulatory elements in the region associated with exon 1 (promoter 1). In contrast, several elements contained in the regulatory regions of other T cell-specific genes, such as ets, lyf-1 and ikaros were found in in promoter 2. Analysis of RT6-transcripts showed this region to be the most active promoter in spleen cells of adult rats. Finally, transient transfection assays with reporter gene constructs showed promoter 2 to mediate T-cell specific transcription.

[1]  S. Smale,et al.  LyF-1, a transcriptional regulator that interacts with a novel class of promoters for lymphocyte-specific genes , 1991, Molecular and cellular biology.

[2]  D. Greiner,et al.  Absence of the RT-6 T cell subset in diabetes-prone BB/W rats. , 1986, Journal of immunology.

[3]  R. Schwinzer,et al.  Expression of RT6 alloantigens and the T-cell receptor on intestinal intraepithelial lymphocytes of the rat. , 1990, Transplantation proceedings.

[4]  H. Gaskins,et al.  Defects in the structure and expression of the genes for the T cell marker Rt6 in NZW and (NZB x NZW)F1 mice. , 1995, International immunology.

[5]  J. Moss,et al.  Molecular characterization of NAD:arginine ADP-ribosyltransferase from rabbit skeletal muscle. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[6]  T. Mak,et al.  Structure of the two promoters of the human lck gene: differential accumulation of two classes of lck transcripts in T cells. , 1989, Molecular and cellular biology.

[7]  K. Iida,et al.  Expression of NAD glycohydrolase activity by rat mammary adenocarcinoma cells transformed with rat T cell alloantigen RT6.2. , 1994, The Journal of biological chemistry.

[8]  H. Hurst,et al.  Transcriptional repression by a novel member of the bZIP family of transcription factors , 1992, Molecular and cellular biology.

[9]  D. Klatzmann,et al.  Characterization of the human CD4 gene promoter: transcription from the CD4 gene core promoter is tissue-specific and is activated by Ets proteins. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[10]  K. Georgopoulos,et al.  The Ikaros gene encodes a family of functionally diverse zinc finger DNA-binding proteins , 1994, Molecular and cellular biology.

[11]  F. Koch-Nolte,et al.  Both allelic forms of the rat T cell differentiation marker RT6 display nicotinamide adenine dinucleotide (NAD)‐glycohydrolase activity, yet only RT6.2 is capable of automodification upon incubation with NAD , 1995, European journal of immunology.

[12]  T. Taniguchi,et al.  Recognition DNA sequences of interferon regulatory factor 1 (IRF-1) and IRF-2, regulators of cell growth and the interferon system , 1993, Molecular and cellular biology.

[13]  F. Haag,et al.  Primary structure of rat RT6.2, a nonglycosylated phosphatidylinositol-linked surface marker of postthymic T cells. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[14]  Holger Karas,et al.  TRANSFAC: a database on transcription factors and their DNA binding sites , 1996, Nucleic Acids Res..

[15]  E Wingender,et al.  Structure of the gene encoding the rat T cell ecto-ADP-ribosyltransferase RT6. , 1996, Journal of immunology.