Single Live Cell Monitoring of Protein Turnover Reveals Intercellular Variability and Cell-Cycle Dependence of Degradation Rates.

[1]  D. Suter,et al.  Endogenous fluctuations of OCT4 and SOX2 bias pluripotent cell fate decisions , 2018, bioRxiv.

[2]  D. Suter,et al.  Single-Cell Quantification of Protein Degradation Rates by Time-Lapse Fluorescence Microscopy in Adherent Cell Culture. , 2018, Journal of visualized experiments : JoVE.

[3]  P. Cluzel,et al.  Systematic characterization of maturation time of fluorescent proteins in living cells , 2017, Nature Methods.

[4]  A. Raj,et al.  Mitotic transcription and waves of gene reactivation during mitotic exit , 2017, Science.

[5]  U. Alon,et al.  An Endogenously Tagged Fluorescent Fusion Protein Library in Mouse Embryonic Stem Cells , 2017, Stem cell reports.

[6]  L. Her,et al.  The Ubiquitin Receptor ADRM1 Modulates HAP40-Induced Proteasome Activity , 2016, Molecular Neurobiology.

[7]  S. Manley,et al.  A role for mitotic bookmarking of SOX2 in pluripotency and differentiation , 2016, Genes & development.

[8]  D. Largaespada,et al.  mTORC1 Coordinates Protein Synthesis and Immunoproteasome Formation via PRAS40 to Prevent Accumulation of Protein Stress. , 2016, Molecular cell.

[9]  W. Huber,et al.  TimerQuant: a modelling approach to tandem fluorescent timer design and data interpretation for measuring protein turnover in embryos , 2016, Development.

[10]  J. Weissman,et al.  Regulation of mRNA translation during mitosis , 2015, eLife.

[11]  B. Bukau,et al.  Incomplete proteasomal degradation of green fluorescent proteins in the context of tandem fluorescent protein timers , 2015, bioRxiv.

[12]  W. Huber,et al.  Protein quality control at the inner nuclear membrane , 2014, Nature.

[13]  D. Kwiatkowski,et al.  Coordinated regulation of protein synthesis and degradation by mTORC1 , 2014, Nature.

[14]  Felix Naef,et al.  Robust synchronization of coupled circadian and cell cycle oscillators in single mammalian cells , 2014, Molecular systems biology.

[15]  C. Niehrs,et al.  Mitotic wnt signaling promotes protein stabilization and regulates cell size. , 2014, Molecular cell.

[16]  Felix Naef,et al.  Stimulus-induced modulation of transcriptional bursting in a single mammalian gene , 2013, Proceedings of the National Academy of Sciences.

[17]  Adam B. Olshen,et al.  The translational landscape of the mammalian cell cycle. , 2013, Molecular cell.

[18]  S. Morley,et al.  Phosphorylation of eIF4GII and 4E-BP1 in response to nocodazole treatment: A reappraisal of translation initiation during mitosis , 2013, Cell cycle.

[19]  Tamar Geiger,et al.  Novel proteomic approach (PUNCH-P) reveals cell cycle-specific fluctuations in mRNA translation , 2013, Genes & development.

[20]  Johannes E. Schindelin,et al.  Fiji: an open-source platform for biological-image analysis , 2012, Nature Methods.

[21]  Philipp J. Keller,et al.  Tandem fluorescent protein timers for in vivo analysis of protein dynamics , 2012, Nature Biotechnology.

[22]  Zhiwei Wang,et al.  Protein Degradation in Cell Cycle , 2012 .

[23]  D. Trono,et al.  Measuring in vivo protein half-life. , 2011, Chemistry & biology.

[24]  Nacho Molina,et al.  Mammalian Genes Are Transcribed with Widely Different Bursting Kinetics , 2011, Science.

[25]  M. Frohman,et al.  Rapid amplification of cDNA ends (RACE). , 2011, Methods in molecular biology.

[26]  J. Plouhinec,et al.  Wnt Signaling Requires Sequestration of Glycogen Synthase Kinase 3 inside Multivesicular Endosomes , 2010, Cell.

[27]  R. Milo,et al.  Protein Dynamics in Individual Human Cells: Experiment and Theory , 2009, PloS one.

[28]  R. Milo,et al.  Dynamic Proteomics of Individual Cancer Cells in Response to a Drug , 2008, Science.

[29]  A. Oudenaarden,et al.  Nature, Nurture, or Chance: Stochastic Gene Expression and Its Consequences , 2008, Cell.

[30]  Marjeta Urh,et al.  HaloTag: a novel protein labeling technology for cell imaging and protein analysis. , 2008, ACS chemical biology.

[31]  Michael Z. Lin,et al.  Improving the photostability of bright monomeric orange and red fluorescent proteins , 2008, Nature Methods.

[32]  Hannah H. Chang,et al.  Transcriptome-wide noise controls lineage choice in mammalian progenitor cells , 2008, Nature.

[33]  Uri Alon,et al.  Generation of a fluorescently labeled endogenous protein library in living human cells , 2007, Nature Protocols.

[34]  K. Krause,et al.  Rapid Generation of Stable Transgenic Embryonic Stem Cell Lines Using Modular Lentivectors , 2006, Stem cells.

[35]  T. Elston,et al.  Stochasticity in gene expression: from theories to phenotypes , 2005, Nature Reviews Genetics.

[36]  H. Vogel,et al.  A general method for the covalent labeling of fusion proteins with small molecules in vivo , 2003, Nature Biotechnology.

[37]  C. Spencer,et al.  Mitotic repression of RNA polymerase II transcription is accompanied by release of transcription elongation complexes , 1997, Molecular and cellular biology.

[38]  J. Jarvik,et al.  CD-tagging: a new approach to gene and protein discovery and analysis. , 1996, BioTechniques.

[39]  E. V. Makeyev,et al.  Folding of firefly luciferase during translation in a cell‐free system. , 1994, The EMBO journal.

[40]  S. Rogers,et al.  Amino acid sequences common to rapidly degraded proteins: the PEST hypothesis. , 1986, Science.