Simultaneous Measurement of Neural Spike Recordings and Multi-Photon Calcium Imaging in Neuroblastoma Cells

This paper proposes the design and implementation of a micro-electrode array (MEA) for neuroblastoma cell culturing. It also explains the implementation of a multi-photon microscope (MPM) customized for neuroblastoma cell excitation and imaging under ambient light. Electrical signal and fluorescence images were simultaneously acquired from the neuroblastoma cells on the MEA. MPM calcium images of the cultured neuroblastoma cell on the MEA are presented and also the neural activity was acquired through the MEA recording. A calcium green-1 (CG-1) dextran conjugate of 10,000 D molecular weight was used in this experiment for calcium imaging. This study also evaluated the calcium oscillations and neural spike recording of neuroblastoma cells in an epileptic condition. Based on our observation of neural spikes in neuroblastoma cells with our proposed imaging modality, we report that neuroblastoma cells can be an important model for epileptic activity studies.

[1]  W. Denk,et al.  Imaging in vivo: watching the brain in action , 2008, Nature Reviews Neuroscience.

[2]  H. Lutcke,et al.  Two-photon imaging and analysis of neural network dynamics , 2011, 1102.5528.

[3]  J. Anthony Movshon,et al.  Comparison of Recordings from Microelectrode Arrays and Single Electrodes in the Visual Cortex , 2007, The Journal of Neuroscience.

[4]  K. Svoboda,et al.  Photon Upmanship: Why Multiphoton Imaging Is More than a Gimmick , 1997, Neuron.

[5]  Kamran Khodakhah,et al.  The Therapeutic Mode of Action of 4-Aminopyridine in Cerebellar Ataxia , 2010, The Journal of Neuroscience.

[6]  O. Creutzfeldt,et al.  [Microphysiology of cortical neurons & their role in sensory and cerebral function]. , 1957, Deutsche medizinische Wochenschrift.

[7]  Hanry Yu,et al.  Cell Culture on MEMS Platforms: A Review , 2009, International journal of molecular sciences.

[8]  Woodrow L. Shew,et al.  Simultaneous multi-electrode array recording and two-photon calcium imaging of neural activity , 2010, Journal of Neuroscience Methods.

[9]  D. Hubel,et al.  Receptive fields of single neurones in the cat's striate cortex , 1959, The Journal of physiology.

[10]  W. Webb,et al.  Nonlinear magic: multiphoton microscopy in the biosciences , 2003, Nature Biotechnology.

[11]  G. Buzsáki.  Large-scale recording of neuronal ensembles , 2004, Nature Neuroscience.

[12]  Alexander J. Sadovsky,et al.  Heuristically optimal path scanning for high-speed multiphoton circuit imaging. , 2011, Journal of neurophysiology.

[13]  V. Mountcastle,et al.  Response properties of neurons of cat's somatic sensory cortex to peripheral stimuli. , 1957, Journal of neurophysiology.

[14]  W. Denk,et al.  Deep tissue two-photon microscopy , 2005, Nature Methods.

[15]  Raj Mutharasan,et al.  Cantilever biosensors in drug discovery , 2009, Expert opinion on drug discovery.