Protein adsorption on crosslinked polydimethylsiloxane using total internal reflection fluorescence

Abstract Total internal reflection fluorescence (TIRF) is used to examine the adsorption of bovine serum albumin (BSA) and bovine fibrinogen on crosslinked polydimethylsiloxane (silicone rubber) surfaces from flowing solutions. By comparing experimentally observed adsorption rates with the predictions of a convection/diffusion model, it is shown that the adsorption of both BSA and fibrinogen on silicone rubber is diffusion-controlled over the range of solution concentrations (0.01

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