Ommatidia of blow fly, house fly, and flesh fly: implication of their vision efficiency

This work aims to elucidate the number of ommatidia or facets (the outwardly visible units of each ommatidium) for compound eyes in blow flies [Chrysomya megacephala (F.), Chrysomya rufifacies (Macquart), Chrysomya nigripes (Aubertin), Lucilia cuprina (Wiedemann)], house flies (Musca domestica L.), and flesh flies (Liosarcophaga dux Thomson) by manual counts of the corneal spreads. The head of the fly in each species was soaked in 20% potassium hydroxide solution at room temperature for 7 days, and the clear compound eye was dissected into six small parts, each of which was placed onto a slide and flattened using a coverslip. Images of each part were obtained using a microscope connected to a computer. The printed images of each part were magnified, and the total number of ommatidia per eye was manually counted. For males, the mean number of ommatidia was statistically different among all flies examined: L. dux (6,032) > C. rufifacies (5,356) > C. nigripes (4,798) > C. megacephala (4,376) > L. cuprina (3,665) > M. domestica (3,484). Likewise, the mean number of facets in females was statistically different: L. dux (6,086) > C. megacephala (5,641) > C. rufifacies (5,208) > C. nigripes (4,774) > L. cuprina (3,608) > M. domestica (3433). Scanning electron microscopy analysis of adult flies revealed the sexual dimorphism in the compound eye. Male C. megacephala had large ommatidia in the upper two thirds part and small ommatidia in the lower one third part, whereas only small ommatidia were detected in females. Dense postulate appearance was detected in the external surface of the corneal lens of the ommatidia of C. megacephala, C. rufifacies, and C. nigripes, while a mix of dense postulate appearance and variable groove array length was detected in L. cuprina and M. domestica. The probable functions of ommatidia are discussed with reference to other literature.

[1]  J. Byrd,et al.  Forensic Entomology: The Utility of Arthropods in Legal Investigations , 2000 .

[2]  C. Bernhard,et al.  Comparative ultrastructure of corneal surface topography in insects with aspects on phylogenesis and function , 1970, Zeitschrift für vergleichende Physiologie.

[3]  B. Greenberg,et al.  FLIES AND DISEASE. , 1965, Scientific American.

[4]  S. Shinonaga.  The altitudinal distribution of flies on Mt. Fuji in summer season , 1965 .

[5]  W. Gordon,et al.  Variation of ommatidia number as a function of worker size inCamponotus pennsylvanicus (DeGeer) (Hymenoptera: Formicidae) , 1992, Insectes Sociaux.

[6]  J. Byrd,et al.  Insects of Forensic Importance , 2009, Forensic Entomology.

[7]  B. Greenberg.  Ecology, classification and biotic associations , 1971 .

[8]  H. Kurahashi,et al.  Forensic entomology cases in Thailand: a review of cases from 2000 to 2006 , 2007, Parasitology Research.

[9]  V. Meyer-Rochow,et al.  Fine structure of the compound eye of the fungus beetle Neotriplax lewisi (Coleoptera : Cucujiformia : Erotylidae) , 2006 .

[10]  G. Baker,et al.  Morphology and number of Ommatidia in the compound eyes of Solenopsis invicta, Solenopsis richteri, and their hybrid (Hymenoptera: Formicidae) , 2006 .

[11]  M. Anderson,et al.  Raman-atomic force microscopy of the ommatidial surfaces of Dipteran compound eyes. , 2003, Journal of structural biology.

[12]  Bernard Greenberg,et al.  Flies and Disease, Vol. 1: Ecology, Classification and Biotic Associations , 1971 .

[13]  H. Kurahashi,et al.  External morphology of Chrysomya pinguis (Walker) (Diptera: Calliphoridae) revealed by scanning electron microscopy. , 2008, Micron.

[14]  J. Kunich,et al.  Entomology and the Law: Flies as Forensic Indicators , 2002 .

[15]  K. Sukontason,et al.  Comparison between Musca domestica and Chrysomya megacephala as carriers of bacteria in northern Thailand. , 2007, The Southeast Asian journal of tropical medicine and public health.

[16]  Fine structure of the compound eye of Mallada basalis (Neuroptera: Chrysopidae) , 1998 .

[17]  Sexual Dimorphism in Eye Morphology in a Butterfly (Asterocampa leilia; Lepidoptera, Nymphalidae) , 1997 .

[18]  K. Sukontason,et al.  Survey of forensically-relevant fly species in Chiang Mai, Northern Thailand. , 2003, Journal of vector ecology : journal of the Society for Vector Ecology.

[19]  W. Tumrasvin.  Studies on medically important flies in Thailand , 1980 .

[20]  Cornelia Fermüller,et al.  Depth estimation using the compound eye of dipteran flies , 2006, Biological Cybernetics.

[21]  S. Wada.  Spezielle randzonale ommatidien von Calliphora Erythrocephala meig. (diptera calliphoridae): Architektur der zentralen rhabdomeren-kolumne und topographie im komplexauge , 1974 .

[22]  E. Buschbeck,et al.  The compound lens eye of Strepsiptera: morphological development of larvae and pupae , 2005 .

[23]  B. Greenberg.  Flics and disease. Vol. II. II. Biology and disease transmission. , 1973 .

[24]  Fritz Zumpt,et al.  Myiasis in Man and Animals in the Old World. A Textbook for Physicians, Veterinarians and Zoologists. , 1965 .

[25]  S. Sucharit,et al.  Studies on medically important flies in Thailand. IV. Altitudinal distribution of flies belonging to Muscidae and Calliphoridae in Doi Indhanondh Mountain, Chiengmai, in early summer season. , 1978, The Bulletin of Tokyo Medical and Dental University.

[26]  E. Warrant,et al.  The eyes of Macrosoma sp. (Lepidoptera: Hedyloidea): a nocturnal butterfly with superposition optics. , 2007, Arthropod structure & development.

[27]  R. Jander,et al.  Allometry and resolution of bee eyes (Apoidea). , 2002, Arthropod structure & development.

[28]  Robin Andrews,et al.  The Science of Entomology , 1975 .

[29]  R. Chapman.  The Insects: Structure and Function , 1969 .

[30]  Richard P. Lane,et al.  Medical Insects and Arachnids , 1993, Springer Netherlands.

[31]  R. G. Davies,et al.  Imms' General textbook of entomology , 1977 .

[32]  An unusual type of corneal nipple in the earwig, forficula sp., with a possible anti-reflection role , 1992 .

[33]  G. Anderson,et al.  Insect Succession on Carrion and Its Relationship to Determining Time of Death , 2000 .

[34]  Kenneth G. V. Smith.  A manual of forensic entomology , 1988 .

[35]  J. Edeson,et al.  The role of non-biting flies in the transmission of enteric pathogens (Salmonella species and Shigella species) in Beirut, Lebanon. , 1978, Annals of tropical medicine and parasitology.

[36]  P. Brandmayr,et al.  Morphometry and eye morphology in three species of Carabus (Coleoptera: Carabidae) in relation to habitat demands , 2007 .

[37]  H. L. Lee,et al.  Mechanical transport of rotavirus by the legs and wings of Musca domestica (Diptera: Muscidae). , 1997, Journal of medical entomology.

[38]  B. Greenberg.  Flies as forensic indicators. , 1991, Journal of medical entomology.

[39]  V. Meyer-Rochow,et al.  Structure and putative function of dark- and light-adapted as well as UV-exposed eyes of the food store pest Psyllipsocus ramburi Sélys-longchamps (Insecta: Psocoptera: Psyllipsocidae). , 2007, Journal of insect physiology.

[40]  D. Burkhardt,et al.  Fine structure of the ommatidia and the occurrence of rhabdomeric twist in the dorsal eye of male Bibio marci (Diptera, Nematocera, Bibionidae) , 2004, Cell and Tissue Research.

[41]  R. Jander,et al.  Numerical allometric growth of the ommatidia, antennal sensilla, and teeth of foretibial combs in the milkweed bug Oncopeltus fasciatus Dallas (Heteroptera: Lygaeidae) , 1994 .

[42]  R. Ngern-klun,et al.  Differentiation of the Third Instar of Forensically Important Fly Species in Thailand , 2004 .

[43]  R. J. Elzinga.  Fundamentals of entomology , 1978 .

[44]  R. G. Davies,et al.  Imms' general textbook of entomology. Tenth edition. Volume 1: structure, physiology and development. Volume 2: classification and biology. , 1977 .

[45]  W. Choochote,et al.  Seasonal fluctuations in populations of the two most forensically important fly species in northern Thailand , 2003, Annals of tropical medicine and parasitology.

[46]  M. L. Goff.  A fly for the prosecution: how insect evidence helps solve crimes. , 2000 .