Behavior of Cell in Uniform Shear Flow Field between Rotating Cone and Stationary Plate

The behavior of biological cell in the uniform shear flow between the rotating cone and the stationary plate has been observed in vitro. The cone and plate device is mounted on the stage of an inverted phase contrast microscope to observe cells adhered on the plate under the shear flow. The deformation and the migration of cells during exposure to the shear flow for 24 hours were traced at the time lapse images. Two kinds of cells were used in the test: C2C12 (mouse myoblast cell line), and HUVEC (human umbilical vein endothelial cells). The experimental results show that HUVEC tends to migrate to the downstream at the shear stress of 1 Pa. C2C12 tends to tilt to the perpendicular direction of the flow for 18 hours between 1 Pa and 2 Pa. HUVEC tends to be rounded at the shear stress of 2 Pa. The behavior of each isolated cell in the shear flow field has been able to be observed quantitatively by the cone and plate device in vitro.

[1]  S. Hashimoto,et al.  Effect of Ultrasonic Vibration on Proliferation and Differentiation of Cells , 2016 .

[2]  Min-Ah Koo,et al.  Stimulated migration and penetration of vascular endothelial cells into poly (L-lactic acid) scaffolds under flow conditions , 2014, Biomaterials Research.

[3]  Guoqing Hu,et al.  A Microfluidic-Based Multi-Shear Device for Investigating the Effects of Low Fluid-Induced Stresses on Osteoblasts , 2014, PloS one.

[4]  Semion K. Saikin,et al.  The nucleus of endothelial cell as a sensor of blood flow direction , 2013, Biology Open.

[5]  Jianping Fu,et al.  Elastomeric microposts integrated into microfluidics for flow-mediated endothelial mechanotransduction analysis. , 2012, Lab on a chip.

[6]  Jeremy J Mao,et al.  Shear stress induces osteogenic differentiation of human mesenchymal stem cells. , 2010, Regenerative medicine.

[7]  S. Hashimoto.  Erythrocyte destruction under periodically fluctuating shear rate: comparative study with constant shear rate. , 2008, Artificial organs.

[8]  Dana M Spence,et al.  Fluorescence monitoring of ATP-stimulated, endothelium-derived nitric oxide production in channels of a poly(dimethylsiloxane)-based microfluidic device. , 2006, Analytical chemistry.

[9]  M. Nieuwdorp,et al.  Vasculoprotective properties of the endothelial glycocalyx: effects of fluid shear stress , 2006, Journal of internal medicine.

[10]  E S Grood,et al.  Alignment and proliferation of MC3T3-E1 osteoblasts in microgrooved silicone substrata subjected to cyclic stretching. , 2000, Journal of biomechanics.

[11]  S. Hashimoto,et al.  Clot growth under periodically fluctuating shear rate. , 1994, Biorheology.

[12]  S. Hashimoto,et al.  Effect of shear rate on clot growth at foreign surfaces. , 1985, Artificial organs.

[13]  R M Nerem,et al.  The elongation and orientation of cultured endothelial cells in response to shear stress. , 1985, Journal of biomechanical engineering.

[14]  S. Hashimoto,et al.  Effect of Shear Stress in Flow on Cultured Cell : Using Rotating Disk at Microscope , 2017 .

[15]  S. Hashimoto,et al.  Effect of Couette Type of Shear Flow by Rotating Disk on Migration of Cell , 2017 .

[16]  S. Hashimoto,et al.  Effect of Flow on Cultured Cell at Micro-pattern of Ridge Lines , 2017 .

[17]  S. Hashimoto,et al.  Design of Cross Type of Flow Channel to Control Orientation of Cell , 2016 .

[18]  S. Hashimoto,et al.  Deformation of Cell Passing through Micro Slit between Micro Ridges , 2016 .

[19]  Shigehiro.  Detect of Sublethal Damage with Cyclic Deformation of Erythrocyte in Shear Flow , 2014 .

[20]  S. Hashimoto,et al.  Effect of Micro Ridges on Orientation of Cultured Cell , 2014 .

[21]  S. Hashimoto,et al.  Effect of Flow Stimulation on Cultured Osteoblast , 2014 .

[22]  S. Hashimoto,et al.  Responses of Cells to Flow in Vitro , 2013 .

[23]  S. Hashimoto,et al.  Observation of Biological Cells in Rhombus Parallelepiped Flow Channel , 2013 .

[24]  Joachim P Spatz,et al.  Myoblast morphology and organization on biochemically micro-patterned hydrogel coatings under cyclic mechanical strain. , 2010, Biomaterials.

[25]  S. Hashimoto,et al.  Effect of Medium Flow on Cultured Cells , 2010 .

[26]  S. Hashimoto,et al.  Effect of Aging on Deformability of Erythrocytes in Shear Flow , 2006 .

[27]  K. Hayakawa,et al.  Orientation of Smooth Muscle-Derived A10 Cells in Culture by Cyclic Stretching: Relationship between Stress Fiber Rearrangement and Cell Reorientation. , 2000, Zoological science.