Aspergillus‐PCR in bronchoalveolar lavage ‐ diagnostic accuracy for invasive pulmonary aspergillosis in critically ill patients

The diagnosis of invasive pulmonary aspergillosis (IPA) in intensive care unit (ICU) patients is challenging, and the role of Aspergillus‐PCR in bronchoalveolar lavage (BAL) is unknown.

[1]  P. Pelosi,et al.  Risk factors and outcome of pulmonary aspergillosis in critically ill coronavirus disease 2019 patients—a multinational observational study by the European Confederation of Medical Mycology , 2021, Clinical Microbiology and Infection.

[2]  T. Calandra,et al.  EORTC/MSGERC Definitions of Invasive Fungal Diseases: Summary of Activities of the Intensive Care Unit Working Group. , 2021, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[3]  E. Kuijper,et al.  Systematic screening for COVID‐19 associated invasive aspergillosis in ICU patients by culture and PCR on tracheal aspirate , 2021, Mycoses.

[4]  O. Bilevich,et al.  COVID19 associated pulmonary aspergillosis , 2021, Clinical Microbiology and Antimicrobial Chemotherapy.

[5]  J. Perfect,et al.  Defining and managing COVID-19-associated pulmonary aspergillosis: the 2020 ECMM/ISHAM consensus criteria for research and clinical guidance , 2020, The Lancet Infectious Diseases.

[6]  M. Lederlin,et al.  Is the COVID-19 Pandemic a Good Time to Include Aspergillus Molecular Detection to Categorize Aspergillosis in ICU Patients? A Monocentric Experience , 2020, Journal of fungi.

[7]  Russell E. Lewis,et al.  Review of influenza-associated pulmonary aspergillosis in ICU patients and proposal for a case definition: an expert opinion , 2020, Intensive Care Medicine.

[8]  T. Qu,et al.  Clinical characteristics of invasive pulmonary aspergillosis in patients with COVID-19 in Zhejiang, China: a retrospective case series , 2020, Critical Care.

[9]  B. Mégarbane,et al.  Prevalence of putative invasive pulmonary aspergillosis in critically ill patients with COVID-19 , 2020, The Lancet Respiratory Medicine.

[10]  M. Hallek,et al.  COVID‐19 associated pulmonary aspergillosis , 2020, Mycoses.

[11]  M. Bassetti,et al.  Performance of existing definitions and tests for the diagnosis of invasive aspergillosis in critically ill, adult patients: a systematic review with qualitative evidence synthesis. , 2020, The Journal of infection.

[12]  J. Perfect,et al.  Revision and Update of the Consensus Definitions of Invasive Fungal Disease From the European Organization for Research and Treatment of Cancer and the Mycoses Study Group Education and Research Consortium , 2019, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[13]  P. Kreidl,et al.  Candida in the Respiratory Tract Potentially Triggers Galactomannan Positivity in Nonhematological Patients , 2019, Antimicrobial Agents and Chemotherapy.

[14]  C. Viscoli,et al.  Use of Aspergillus fumigatus real-time PCR in bronchoalveolar lavage samples (BAL) for diagnosis of invasive aspergillosis, including azole-resistant cases, in high risk haematology patients: the need for a combined use with galactomannan , 2019, Medical mycology.

[15]  C. Breynaert,et al.  Invasive pulmonary aspergillosis complicating severe influenza: epidemiology, diagnosis and treatment , 2018, Current opinion in infectious diseases.

[16]  E. Andrinopoulou,et al.  Invasive aspergillosis in patients admitted to the intensive care unit with severe influenza: a retrospective cohort study. , 2018, The Lancet. Respiratory medicine.

[17]  P. Meybohm,et al.  Detection of invasive pulmonary aspergillosis in critically ill patients by combined use of conventional culture, galactomannan, 1‐3‐beta‐D‐glucan and Aspergillus specific nested polymerase chain reaction in a prospective pilot study , 2018, Journal of critical care.

[18]  O. Dekkers,et al.  The burden of Invasive Aspergillosis in patients with haematological malignancy: A meta-analysis and systematic review. , 2018, The Journal of infection.

[19]  J. Cornelissen,et al.  PCR-based detection of Aspergillus fumigatus Cyp51A mutations on bronchoalveolar lavage: a multicentre validation of the AsperGenius assay® in 201 patients with haematological disease suspected for invasive aspergillosis. , 2016, The Journal of antimicrobial chemotherapy.

[20]  G. Simons,et al.  Validation of a New Aspergillus Real-Time PCR Assay for Direct Detection of Aspergillus and Azole Resistance of Aspergillus fumigatus on Bronchoalveolar Lavage Fluid , 2015, Journal of Clinical Microbiology.

[21]  J. Rello,et al.  A clinical algorithm to diagnose invasive pulmonary aspergillosis in critically ill patients. , 2012, American journal of respiratory and critical care medicine.

[22]  L. Leibovici,et al.  Diagnostic Accuracy of PCR Alone Compared to Galactomannan in Bronchoalveolar Lavage Fluid for Diagnosis of Invasive Pulmonary Aspergillosis: a Systematic Review , 2012, Journal of Clinical Microbiology.

[23]  P. White,et al.  An update on the molecular diagnosis of invasive fungal disease. , 2009, FEMS microbiology letters.

[24]  C. Viscoli,et al.  Invasive aspergillosis: diagnosis, prophylaxis and treatment , 2008, Current opinion in hematology.

[25]  Patricia Muñoz,et al.  Revised definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) Consensus Group. , 2008, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[26]  D. Denning,et al.  Defining opportunistic invasive fungal infections in immunocompromised patients with cancer and hematopoietic stem cell transplants: an international consensus. , 2002, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.