Gas-phase spectroscopy of a protein

We present preliminary results on gas phase photoionization of electrospray-produced multiply protonated cytochrome c protein (104 amino acids;≈12.4 kDa), which has been achieved with an experimental system for spectroscopy of electrosprayed ions in the ion trap using synchrotron radiation. The present results are the first reported on photoionization of kDa species in the gas phase and are valuable regarding fundamental interest of accessing physical properties of large biological species isolated in vacuo.