论文信息 - Observation of terahertz absorption signatures in microliter DNA solutions

Observation of terahertz absorption signatures in microliter DNA solutions

Strong, repeatable Terahertz (THz) signatures are measured in small samples of DNA solution using a THz photomixing spectrometer with custom conical-horn coupling. The DNA specimen is linear, single-stranded (ss), 13-mer polynucleotide, and is diluted in buffer solution to have a concentration of 0.15 μmol/ml. The strongest signature is centered ∼717 GHz, and has a strength of 2.5 × 1016 cm−1/mol, linewidth 48 GHz, and damping time of 6.6 ps. The origin of the signature is attributed to the hydrogen bond of locally cross-linked ssDNAs.

[1] B. Gelmont,et al. THz-Spectroscopy of Biological Molecules , 2003, Journal of biological physics.

[2] Edgar A. Mendoza,et al. High-Resolution THz Spectroscopy to Measure Strong THz Absorption Signatures of si-RNA in Solution , 2011 .

[3] K. Chou,et al. The biological functions of low-frequency vibrations (phonons) 5. A phenomenological theory. , 1985, Biophysical chemistry.

[4] J L Hesler,et al. Submillimeter-wave phonon modes in DNA macromolecules. , 2002, Physical review. E, Statistical, nonlinear, and soft matter physics.

[5] Jagdish K. Vij,et al. Submillimetre wave spectroscopy of 4-n-alkyl-4′-cyano biphenyl liquid crystals , 1989 .

[6] C. Cantor,et al. DNA conformation on surfaces measured by fluorescence self-interference. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[7] Weidong Zhang,et al. Concentration methods for high-resolution THz spectroscopy of nucleic-acid biomolecules and crystals , 2012, BiOS.

[8] Kremer,et al. Far-infrared spectroscopy on oriented films of dry and hydrated DNA. , 1986, Physical review. A, General physics.

[9] Steven A. Corcelli,et al. Molecular Dynamics Simulations of DNA Solvation Dynamics , 2010 .

[10] E. Clementi,et al. Simulations of the solvent structure for macromolecules. III. Determination of the Na+ counter ion structure , 1982, Biopolymers.