Morphometric and dendritic analysis of fascia dentata granule cells in human aging and senile dementia

In this study the cellular morphology in the human fascia dentata of 5 very old demented cases (4 Alzheimer's disease and 1 multi-infarct dementia patients) was compared with 5 (very) old controls cases. The postmortem delay in fixation was for all cases within 3.5 h. In the demented group, a significant reduction in thickness of the molecular layer, density of dendritic spines in the middle third of the molecular layer and total dendritic length (+/- 30%) was found. The number of dendritic segments, indicative of the branching frequency showed no difference. In both the control and the demented group, three-quarters of all dendritic bifurcations of granule cells occurred in the inner third of the molecular layer in which the commissural and associational fibers terminate. The size of the dendrites in the demented group could be the result of at least 3 independent processes: a regressive change due to partial denervation of the outer two-thirds of the molecular layer as axons from the perforant pathway are lost; a dendritic regrowth in response to sprouting of the commissural-associational fiber systems and septal afferents, which is presumed to occur in response to degeneration of perforant path axons; a dendritic regrowth in response to the loss of the dendrites of neighboring cells which have died. Analysis of our material suggests that dendritic degeneration is the predominant factor in the demented group.

[1]  A. Damasio,et al.  Chapter 20 Cell-specific pathology in neural systems of the temporal lobe in Alzheimer's disease , 1986 .

[2]  H. Gundersen.  Stereology of arbitrary particles * , 1986, Journal of microscopy.

[3]  P. Coleman,et al.  Dendritic proliferation in the aging brain as a compensatory repair mechanism. , 1986, Progress in brain research.

[4]  M. Hofman,et al.  Morphometry of size/volume variables and comparison of their bivariate relations in the nervous system under different conditions , 1986, Journal of Neuroscience Methods.

[5]  Paul D. Coleman,et al.  Age-related dendritic growth in dentate gyrus of human brain is followed by regression in the ‘oldest old’ , 1985, Brain Research.

[6]  S. J. Buell.  Golgi‐Cox and Rapid Golgi Methods as Applied to Autopsied Human Brain Tissue: Widely Disparate Results , 1982, Journal of neuropathology and experimental neurology.

[7]  D. Mann,et al.  Some morphometric observations on the cerebral cortex and hippocampus in presenile Alzheimer's disease, senile dementia of Alzheimer type and Down's syndrome in middle age , 1985, Journal of the Neurological Sciences.

[8]  R. S. Williams,et al.  THE GOLGI RAPID METHOD IN CLINICAL NEUROPATHOLOGY: THE MORPHOLOGIC CONSEQUENCES OF SUBOPTIMAL FIXATION , 1978, Journal of neuropathology and experimental neurology.

[9]  Jan Voogd,et al.  The human central nervous system : a synopsis and atlas , 1978 .

[10]  Arnold B. Scheibel,et al.  Quantitative analysis of dendritic branching pattern of granular cells from human dentate gyrus , 1976, Experimental Neurology.

[11]  P. Coleman,et al.  Dendritic extent in human dentate gyrus granule cells in normal aging and senile dementia , 1987, Brain Research.

[12]  William B. Levy,et al.  Granule cell dendritic spine density in the rat hippocampus varies with spine shape and location , 1985, Neuroscience Letters.

[13]  J. D. Ruiter.  The influence of post-mortem fixation delay on the reliability of the Golgi silver impregnation , 1983, Brain Research.

[14]  G. V. Van Hoesen,et al.  Alzheimer's disease: cell-specific pathology isolates the hippocampal formation. , 1984, Science.

[15]  R. Katzman.,et al.  Pathological verification of ischemic score in differentiation of dementias , 1980, Annals of neurology.

[16]  C. P. Hughes,et al.  Mild senile dementia of Alzheimer type: research diagnostic criteria, recruitment, and description of a study population. , 1982, Journal of neurology, neurosurgery, and psychiatry.

[17]  C. P. Hughes,et al.  A New Clinical Scale for the Staging of Dementia , 1982, British Journal of Psychiatry.

[18]  Haase Gr,et al.  Diseases presenting as dementia. , 1971 .

[19]  C. Ribak,et al.  Five types of basket cell in the hippocampal dentate gyrus: a combined Golgi and electron microscopic study , 1983, Journal of neurocytology.

[20]  Paul D. Coleman,et al.  Quantitative evidence for selective dendritic growth in normal human aging but not in senile dementia , 1981, Brain Research.

[21]  M. Mishkin.  A memory system in the monkey. , 1982, Philosophical transactions of the Royal Society of London. Series B, Biological sciences.

[22]  Uylings Hb,et al.  Ordering methods in quantitative analysis of branching structures of dendritic trees. , 1975 .

[23]  C. Cotman,et al.  Plasticity of hippocampal circuitry in Alzheimer's disease. , 1985, Science.

[24]  J. Cammermeyer,et al.  Artifactual displacement of neuronal nucleoli in paraffin sections. , 1967, Journal fur Hirnforschung.

[25]  H. Uylings,et al.  Morphometry of cortical dendrites. , 1981, Progress in clinical and biological research.

[26]  G. W. Hoesen,et al.  Neural Systems of the Non‐human Primate Forebrain Implicated in Memory a , 1985 .

[27]  R. S. Williams,et al.  Age-related changes in Down syndrome brain and the cellular pathology of Alzheimer disease. , 1986, Progress in brain research.

[28]  H. Vahlsing,et al.  Basal lamina at the site of spinal cord injury in normal, immunotolerant and immunosuppressed rats , 1985, Neuroscience Letters.

[29]  W. J. Conover,et al.  Practical Nonparametric Statistics , 1972 .

[30]  W. Penfield,et al.  Memory. Autopsy findings and comments on the role of hippocampus in experiential recall. , 1974, Archives of neurology.

[31]  R. DeTeresa,et al.  Some morphometric aspects of the brain in senile dementia of the alzheimer type , 1981, Annals of neurology.

[32]  H. J. G. Gundersen,et al.  The impact of recent stereological advances on quantitative studies of the nervous system , 1986, Journal of Neuroscience Methods.

[33]  K. Kuypers,et al.  An economical, semi‐automatic system for measuring cellular tree structures in three dimensions, with special emphasis on Golgi‐impregnated neurons , 1978, Journal of microscopy.

[34]  H. B. M. Uylings,et al.  The metric analysis of three-dimensional dendritic tree patterns: a methodological review , 1986, Journal of Neuroscience Methods.

[35]  M. Roth,et al.  The Association Between Quantitative Measures of Dementia and of Senile Change in the Cerebral Grey Matter of Elderly Subjects , 1968, British Journal of Psychiatry.

[36]  L. Nadel,et al.  The Hippocampus as a Cognitive Map , 1978 .

[37]  B. Winblad,et al.  Dementia of the Alzheimer Type and Multi‐infarct Dementia , 1984, Journal of the American Geriatrics Society.

[38]  M. Berry,et al.  Dendritic growth and the control of neuronal form. , 1980, Current topics in developmental biology.