Single-Shot Quantitative Polarization Imaging of Complex Birefringent Structure Dynamics.

Polarization light microscopes are powerful tools for probing molecular order and orientation in birefringent materials. While a number of polarization microscopy techniques are available to access steady-state properties of birefringent samples, quantitative measurements of the molecular orientation dynamics on the millisecond time scale have remained a challenge. We propose polarized shearing interference microscopy (PSIM), a single-shot quantitative polarization imaging method, for extracting the retardance and orientation angle of the laser beam transmitting through optically anisotropic specimens with complex structures. The measurement accuracy and imaging performance of PSIM are validated by imaging a birefringent resolution target and a bovine tendon specimen. We demonstrate that PSIM can quantify the dynamics of a flowing lyotropic chromonic liquid crystal in a microfluidic channel at an imaging speed of 506 frames per second (only limited by the camera frame rate), with a field-of-view of up to 350 × 350 μm2 and a diffraction-limit spatial resolution of ~2 μm. We envision that PSIM will find a broad range of applications in quantitative material characterization under dynamical conditions.

[1]  Z. Yaqoob,et al.  Structures and topological defects in pressure-driven lyotropic chromonic liquid crystals , 2021, Proceedings of the National Academy of Sciences.

[2]  A. Sharma,et al.  Time Dependent Lyotropic Chromonic Textures in Microfluidic Confinements , 2020, Crystals.

[3]  Ruipeng Li,et al.  Shear-induced polydomain structures of nematic lyotropic chromonic liquid crystal disodium cromoglycate. , 2020, Soft matter.

[4]  Daniel A. Beller,et al.  Topological structure and dynamics of three-dimensional active nematics , 2019, Science.

[5]  Hanry Yu,et al.  Studying nucleic envelope and plasma membrane mechanics of eukaryotic cells using confocal reflectance interferometric microscopy , 2019, Nature Communications.

[6]  Ning-Jiun Jan,et al.  Polarized light microscopy for 3‐dimensional mapping of collagen fiber architecture in ocular tissues , 2018, Journal of biophotonics.

[7]  Peter T. C. So,et al.  Reference-free polarization-sensitive quantitative phase imaging using single-point optical phase conjugation. , 2018, Optics express.

[8]  P. So,et al.  Single‐Shot Optical Anisotropy Imaging with Quantitative Polarization Interference Microscopy , 2018, Laser & photonics reviews.

[9]  I. Aranson,et al.  Dynamic states of swimming bacteria in a nematic liquid crystal cell with homeotropic alignment , 2017 .

[10]  I. Aranson,et al.  Topological Defects in a Living Nematic Ensnare Swimming Bacteria , 2017 .

[11]  Shalin B. Mehta,et al.  Dissection of molecular assembly dynamics by tracking orientation and position of single molecules in live cells , 2016, Proceedings of the National Academy of Sciences.

[12]  S. Zhuang,et al.  Parallel detection experiment of fluorescence confocal microscopy using DMD. , 2016, Scanning.

[13]  O. Lavrentovich,et al.  Elasticity, viscosity, and orientational fluctuations of a lyotropic chromonic nematic liquid crystal disodium cromoglycate. , 2014, Soft matter.

[14]  Yukitoshi Otani,et al.  A development of two-dimensional birefringence distribution measurement system with a sampling rate of 1.3 MHz , 2014 .

[15]  S. D. Babacan,et al.  White-light diffraction tomography of unlabelled live cells , 2014, Nature Photonics.

[16]  I. Aranson,et al.  Living liquid crystals , 2013, Proceedings of the National Academy of Sciences.

[17]  R. Oldenbourg.  Polarized light microscopy: principles and practice. , 2013, Cold Spring Harbor protocols.

[18]  S. Herminghaus,et al.  Liquid crystal microfluidics for tunable flow shaping. , 2013, Physical review letters.

[19]  C. Bahr,et al.  Functionalization of microfluidic devices for investigation of liquid crystal flows , 2012 .

[20]  C. Depeursinge,et al.  Quantitative phase imaging in biomedicine , 2012, 2012 Conference on Lasers and Electro-Optics (CLEO).

[21]  Samantha J. Stehbens,et al.  Imaging intracellular protein dynamics by spinning disk confocal microscopy. , 2012, Methods in enzymology.

[22]  I. Smalyukh,et al.  Optical Microscopy of Soft Matter Systems , 2011, 1108.3287.

[23]  Zhuo Wang,et al.  Measuring the scattering parameters of tissues from quantitative phase imaging of thin slices. , 2011, Optics letters.

[24]  R. Hurt,et al.  Vertically Aligned Graphene Layer Arrays from Chromonic Liquid Crystal Precursors , 2011, Advanced materials.

[25]  O. Lavrentovich,et al.  Lyotropic chromonic liquid crystal semiconductors for water-solution processable organic electronics , 2010, 1101.0742.

[26]  Zhuo Wang,et al.  Jones phase microscopy of transparent and anisotropic samples. , 2008, Optics letters.

[27]  Ralf Wolleschensky,et al.  High-speed confocal fluorescence imaging with a novel line scanning microscope. , 2006, Journal of biomedical optics.

[28]  Shin-Tson Wu,et al.  Fundamentals of Liquid Crystal Devices: Yang/Fundamentals of Liquid Crystal Devices , 2006 .

[29]  O. Lavrentovich,et al.  Optical characterization of the nematic lyotropic chromonic liquid crystals: light absorption, birefringence, and scalar order parameter. , 2005, Physical review. E, Statistical, nonlinear, and soft matter physics.

[30]  Etienne Cuche,et al.  Polarization microscopy by use of digital holography: application to optical-fiber birefringence measurements. , 2005, Applied optics.

[31]  O. Lavrentovich,et al.  Lyotropic Chromonic Liquid Crystals for Biological Sensing Applications , 2005 .

[32]  Michael Shribak,et al.  Mapping polymer birefringence in 3D using a polarizing microscope with oblique illumination , 2004, SPIE Photonics Europe.

[33]  S. Casey,et al.  Molecularly Designed Chromonic Liquid Crystals for the Fabrication of Broad Spectrum Polarizing Materials , 2004 .

[34]  Rudolf Oldenbourg,et al.  Polarization microscopy with the LC-PolScope , 2003 .

[35]  O. Lavrentovich.  Fluorescence confocal polarizing microscopy: Three-dimensional imaging of the director , 2003 .

[36]  Michael Shribak,et al.  Techniques for fast and sensitive measurements of two-dimensional birefringence distributions. , 2003, Applied optics.

[37]  J. Kováč,et al.  Structural and magnetic properties of metastable Fe1-xSix (0.15 < x < 0.34) alloys prepared by a rapid-quenching technique , 2002 .

[38]  Oleg D. Lavrentovich,et al.  Three-dimensional imaging of orientational order by fluorescence confocal polarizing microscopy , 2001 .

[39]  S Inoué,et al.  Microtubule dynamics in mitotic spindle displayed by polarized light microscopy. , 1998, Molecular biology of the cell.

[40]  R. Oldenbourg.  A new view on polarization microscopy , 1996, Nature.

[41]  C. Werner,et al.  Satellite radar interferometry: Two-dimensional phase unwrapping , 1988 .

[42]  Salvatore J. Rodano,et al.  Elasticity of glass , 1979 .

[43]  Shuang Zhou.  Lyotropic Chromonic Liquid Crystals , 2017 .

[44]  E D Salmon,et al.  Birefringence of single and bundled microtubules. , 1998, Biophysical journal.