SUCROSE‐INDOLE‐3‐ACETIC ACID INTERACTIONS ON ROOT REGENERATION BY PINUS LAMBERTIANA EMBRYO CUTTINGS

A B S T R A C T Interactions affecting root formation by IAA and sucrose, supplied to Pinus lambertiana embryo cuttings through their cotyledons, were explored. The sucrose optimum for rooting and dry weight increase is approximately 8 %. Osmotic substitutes for sucrose, applied alone or concomitantly with sucrose, were unable to duplicate its effects on regeneration. One AM IAA increased the number of roots per cutting with all sucrose concentrations. However, with suboptimal sucrose concentrations it only increased the total number of cuttings forming roots. Because IAA accumulates at the site of root formation, the possibility that it mobilizes sucrose or its derivatives to the site of root formation was explored. No evidence was found for hormone-directed transport of sucrose in this system. IAA had no effect on either the basal accumulation or transport of label derived from U-14C-sucrose. IN A PREVIOUS PAPER Greenwood and Goldsmith (1970) showed that accumulation of polarly transported indole-3-acetic acid (IAA) precedes basal root regeneration by Pinus lambertiana embryo cuttings. The purpose of this paper is to correlate the movement and accumulation of sucrose and its derivatives at the site of root regeneration with the results of similar studies with IAA.

[1]  C. Rizzi Statistical Methods , 2020, Springer Theses.

[2]  R. Ernst,et al.  CARBOHYDRATE PHYSIOLOGY OF ORCHID SEEDLINGS. II. HYDROLYSIS AND EFFECTS OF OLIGOSACCHARIDES , 1971 .

[3]  M. Jain,et al.  Effect of Glucose and Auxins in Rooting Etiolated Stem Segments of Populus nigra , 1971 .

[4]  M. G. Mullins Hormone-directed Transport of Assimilates in Decapitated Internodes of Phaseolus vulgaris L. , 1970 .

[5]  M. Greenwood The developmental physiology and morphology of root regeneration or studies on the regeneration of roots by hypocotyl segments from the dormant, mature embryo of Pinus lambertiana Dougl. , 1970 .

[6]  D. Doley,et al.  EFFECTS OF GROWTH REGULATING SUBSTANCES AND WATER POTENTIAL ON THE DEVELOPMENT OF WOUND CALLUS IN FRAXINUS , 1970 .

[7]  B. E. Michel DIFFERENT SPECIES, SUCROSE, AND LIGHT IN PLANT SECTION ELONGATION TESTS , 1968 .

[8]  Z. Eshhar,et al.  Transport of 3-o-Methylglucose Into and Out of Storage Cells of Daucus carota. , 1968, Plant physiology.

[9]  G. W. Snedecor STATISTICAL METHODS , 1967 .

[10]  P. Wareing,et al.  Hormone-directed Transport of Metabolites and its possible Role in Plant Senescence , 1967 .

[11]  J. Miksche,et al.  GROWTH OF EXCISED PINE EMBRYOS AND THE ROLE OF THE COTYLEDONS DURING GERMINATION IN VITRO , 1965 .

[12]  G. Berlyn,et al.  REGENERATION OF ACTIVE ROOT MERISTEMS IN VITRO BY HYPOCOTYL SECTIONS FROM DORMANT PINUS LAMBERTIANA EMBRYOS , 1965 .

[13]  J. Torrey,et al.  GROWTH AND MORPHOGENESIS OF GLOBULAR AND OLDER EMBRYOS OF CAPSELLA IN CULTURE , 1963 .

[14]  J. G. Kirchner,et al.  Thin Layer Chromatography , 1963 .

[15]  H. Street,et al.  The Effects of Gibberellins on the Growth of Excised Tomato Roots , 1960 .

[16]  H. Street,et al.  The Carbohydrate Nutrition of Tomato Roots , 1958 .

[17]  J. W. Mitchell,et al.  Mineral Composition of Bean Stems Treated with 3-Indoleacetic Acid , 1948, Botanical Gazette.

[18]  S. A. Gordon,et al.  AN ANALYSIS OF THE FUNCTION OF THE LEAF IN THE PROCESS OF ROOT FORMATION IN CUTTINGS , 1946 .

[19]  A. Blakeslee,et al.  CULTIVATION IN VITRO OF SMALL DATURA EMBRYOS , 1942 .