Modeling of the Urothelium with an Agent Based Approach

Novel models for cell differentiation and proliferation in the urothelium are presented. The models are simulated with the Glazier-Graner-Hogeweg technique using CompuCell3D. From a variety of tested models, the contact model is the best candidate to explain cell proliferation in the healthy urothelium. Based on this model, four variations were compared to highlight the key variations that best fit real urothelium. All simulations were quantified by a fitness function designed for the requirements of the urothelium. The findings suggest that adhesion and a nutrient dependent growth may play a crucial role in the maintenance of the urothelium. Aberrations in either adhesion or nutrient dependent growth led to the development of polyp-like formations. This work mimics the regeneration process and the steady state of the urothelium with a spatial and adhesion dependent approach for the first time.

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