Application of fluorescent and vibration spectroscopy for septic serum human albumin structure deformation during pathology

In this paper we perform results of conformational analysis of septic human serum albumin (HSA) carried out by Raman spectroscopy (RS), infrared (IR) spectroscopy and fluorescent spectroscopy. The main vibrational groups were identified and analyzed for septic HSA and its health control. Comparison between Raman and IR results were done. Fluorescent spectral changes of Trp-214 group were analyzed. Application of Raman, IR spectroscopy, fluorescent spectroscopy for conformational changes study of HSA during pathology were shown.

[1]  I. G. Samusev,et al.  Plasmon enhancement of Raman scattering and fluorescence for rhodamine 6G molecules in the porous glass and PVA films with nanoparticles of silver citrate hydrosol , 2016 .

[2]  G. Weber,et al.  Ultraviolet fluorescence of the aromatic amino acids. , 1957, The Biochemical journal.

[3]  Yukihiro Ozaki,et al.  Adsorption of S—S Containing Proteins on a Colloidal Silver Surface Studied by Surface-Enhanced Raman Spectroscopy , 2004, Applied spectroscopy.

[4]  M. Abu-Taha,et al.  Spectroscopic approach of the interaction study of ceftriaxone and human serum albumin , 2014 .

[5]  J. Lakowicz.  Principles of fluorescence spectroscopy , 1983 .

[6]  Werner Schmidt,et al.  Optical spectroscopy in chemistry and life sciences , 2005 .

[7]  Yukihiro Ozaki,et al.  Part I: Surface-Enhanced Raman Spectroscopy Investigation of Amino Acids and Their Homodipeptides Adsorbed on Colloidal Silver , 2004, Applied spectroscopy.

[8]  B. Gaber,et al.  Laser Raman scattering as a probe of protein structure. , 1977, Annual review of biochemistry.

[9]  M. Xie,et al.  Characterization of the interaction between human serum albumin and morin. , 2006, Biochimica et biophysica acta.

[10]  S. Boxer,et al.  Stark effect spectroscopy of tryptophan. , 1995, Biophysical journal.

[11]  J. Kong,et al.  Fourier transform infrared spectroscopic analysis of protein secondary structures. , 2007, Acta biochimica et biophysica Sinica.

[12]  Y. Ozaki,et al.  Surface-Enhanced Raman Spectroscopy , 2005 .

[13]  E. Sikorska,et al.  Raman and surface-enhanced Raman spectroscopy investigation of vasopressin analogues containing 1-aminocyclohexane-1-carboxylic acid residue. , 2006, Biopolymers.

[14]  Zai-Qing Wen,et al.  Raman spectroscopy of protein pharmaceuticals. , 2007, Journal of pharmaceutical sciences.

[15]  H. Možina,et al.  Near-infrared spectroscopy for evaluation of global and skeletal muscle tissue oxygenation. , 2011, World journal of cardiology.

[16]  Sang J. Chung,et al.  Intrinsic Tryptophan Fluorescence in the Detection and Analysis of Proteins: A Focus on Förster Resonance Energy Transfer Techniques , 2014, International journal of molecular sciences.

[17]  P. Vandenabeele,et al.  Reference database of Raman spectra of biological molecules , 2007 .

[18]  Jin Wang,et al.  Affinity and specificity of levamlodipine-human serum albumin interactions: insights into its carrier function. , 2009, Biophysical journal.

[19]  Jürgen Popp,et al.  SERS: a versatile tool in chemical and biochemical diagnostics , 2008, Analytical and bioanalytical chemistry.

[20]  E. Podstawka.  Investigation of molecular structure of bombesin and its modified analogues nonadsorbed and adsorbed on electrochemically roughened silver surface , 2008, Biopolymers.

[21]  Jia-Xin Dong,et al.  Fluorometric investigation of the interaction between methylene blue and human serum albumin. , 2005, Journal of pharmaceutical and biomedical analysis.

[22]  L. Vogler.  Lehninger Principles Of Biochemistry 5th Edition , 2016 .

[23]  Y. Ozaki,et al.  Part II: Surface-Enhanced Raman Spectroscopy Investigation of Methionine Containing Heterodipeptides Adsorbed on Colloidal Silver , 2004, Applied spectroscopy.

[24]  M. Eftink.  Fluorescence techniques for studying protein structure. , 2006, Methods of biochemical analysis.

[25]  Dean E. Myers,et al.  Near-infrared spectroscopy in patients with severe sepsis: correlation with invasive hemodynamic measurements. , 2008, Surgical infections.

[26]  Peter M. Fredericks,et al.  Surface-enhanced Raman spectroscopy of peptides and proteins adsorbed on an electrochemically prepared silver surface , 1999 .

[27]  A. Barth.  Infrared spectroscopy of proteins. , 2007, Biochimica et biophysica acta.

[28]  Eckhard Koglin,et al.  Surface enhanced raman scattering of biomolecules , 1986 .