Interaction between human topoisomerase I and a novel RING finger/arginine-serine protein.

The N-terminus of human topoisomerase I participates in the binding of this enzyme to helicases and other proteins. Using the N-terminal 250 amino acids of human topoisomerase I and a yeast two-hybrid/ in vitro binding screen, a novel arginine-serine-rich peptide was identified as a human topoisomerase I-binding protein. The corresponding full-length protein, named topors, contains a consensus RING zinc finger domain and nuclear localization signals in addition to the arginine-serine-rich region. The RING finger domain of topors is homologous to a similar domain in a family of viral proteins that are involved in the regulation of viral transcription. When expressed in HeLa cells as a green fluorescent protein fusion, topors localizes in the nucleus in a punctate pattern and co-immunoprecipitates with topoisomerase I. These data suggest that topors is involved in trans-cription, possibly recruiting topoisomerase I to RNA polymerase II transcriptional complexes.

[1]  T. Straub,et al.  The RNA-splicing Factor PSF/p54 Controls DNA-Topoisomerase I Activity by a Direct Interaction* , 1998, The Journal of Biological Chemistry.

[2]  C. Panagiotidis,et al.  The NH2 Terminus of the Herpes Simplex Virus Type 1 Regulatory Protein ICP0 Contains a Promoter-Specific Transcription Activation Domain , 1998, Journal of Virology.

[3]  P. Freemont,et al.  The Human Polycomb Group Complex Associates with Pericentromeric Heterochromatin to Form a Novel Nuclear Domain , 1998, The Journal of cell biology.

[4]  D. Simmons,et al.  The Activity of Topoisomerase I Is Modulated by Large T Antigen during Unwinding of the SV40 Origin* , 1998, The Journal of Biological Chemistry.

[5]  P. Haluska,et al.  A role for the amino terminus of human topoisomerase I. , 1998, Advances in enzyme regulation.

[6]  W. Shaiu,et al.  Targeting to Transcriptionally Active Loci by the Hydrophilic N-Terminal Domain of Drosophila DNA Topoisomerase I , 1998, Molecular and Cellular Biology.

[7]  J. Tazi,et al.  Interaction between the N-terminal domain of human DNA topoisomerase I and the arginine-serine domain of its substrate determines phosphorylation of SF2/ASF splicing factor. , 1998, Nucleic acids research.

[8]  P. Haluska,et al.  Interaction between the N-terminus of human topoisomerase I and SV40 large T antigen. , 1998, Nucleic acids research.

[9]  Y. Pommier,et al.  Mammalian DNA topoisomerase I activity and poisoning by camptothecin are inhibited by simian virus 40 large T antigen. , 1998, Biochemistry.

[10]  P. Bouvet,et al.  Nucleolin functions in the first step of ribosomal RNA processing , 1998, The EMBO journal.

[11]  S. Silverstein,et al.  Mutational analysis of the herpes simplex virus type 1 ICP0 C3HC4 zinc ring finger reveals a requirement for ICP0 in the expression of the essential alpha27 gene , 1997, Journal of virology.

[12]  G. Schuler.  Pieces of the puzzle: expressed sequence tags and the catalog of human genes , 1997, Journal of Molecular Medicine.

[13]  R. Jordan,et al.  Activation of gene expression by herpes simplex virus type 1 ICP0 occurs at the level of mRNA synthesis , 1997, Journal of virology.

[14]  Thomas L. Madden,et al.  Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. , 1997, Nucleic acids research.

[15]  Tom Misteli,et al.  The dynamics of a pre-mRNA splicing factor in living cells , 1997, Nature.

[16]  P. Sharp,et al.  Topoisomerase I enhances TFIID-TFIIA complex assembly during activation of transcription. , 1997, Genes & development.

[17]  S. Morham,et al.  Targeted disruption of the mouse topoisomerase I gene by camptothecin selection , 1996, Molecular and cellular biology.

[18]  P. Brzoska,et al.  Mitotic chromosome condensation in the rDNA requires TRF4 and DNA topoisomerase I in Saccharomyces cerevisiae. , 1996, Genes & development.

[19]  B. Stillman,et al.  Simian virus 40 large T antigen binds to topoisomerase I. , 1996, Virology.

[20]  J. Tazi,et al.  Specific phosphorylation of SR proteins by mammalian DNA topoisomerase I , 1996, Nature.

[21]  Y. Shimura,et al.  A human RNA helicase-like protein, HRH1, facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome. , 1996, Genes & development.

[22]  Z. Wang,et al.  Differential control of transcription‐induced and overall DNA supercoiling by eukaryotic topoisomerases in vitro. , 1996, The EMBO journal.

[23]  D. Kufe,et al.  Identification of a Nucleolin Binding Site in Human Topoisomerase I (*) , 1996, The Journal of Biological Chemistry.

[24]  T Pawson,et al.  The Clk/Sty protein kinase phosphorylates SR splicing factors and regulates their intranuclear distribution. , 1996, The EMBO journal.

[25]  R. Everett,et al.  HSV‐1 IE protein Vmw110 causes redistribution of PML. , 1994, The EMBO journal.

[26]  M. Farrer,et al.  The SON gene encodes a conserved DNA binding protein mapping to human chromosome 21 , 1994, Annals of human genetics.

[27]  D. Reinberg,et al.  DNA topoisomerase I is involved in both repression and activation of transcription , 1993, Nature.

[28]  A. Zahler,et al.  Human SR proteins and isolation of a cDNA encoding SRp75 , 1993, Molecular and cellular biology.

[29]  S. Fields,et al.  Elimination of false positives that arise in using the two-hybrid system. , 1993, BioTechniques.

[30]  Y. Cheng,et al.  Characterization of camptothecin-resistant Chinese hamster lung cells. , 1992, Biochemical pharmacology.

[31]  S. Nagpal,et al.  Characterization of a potent varicella-zoster virus-encoded trans-repressor , 1991, Journal of virology.

[32]  D. Kufe,et al.  Activation of the c-jun protooncogene in human myeloid leukemia cells treated with etoposide. , 1991, Molecular pharmacology.

[33]  R. Laskey,et al.  Nuclear targeting sequences--a consensus? , 1991, Trends in biochemical sciences.

[34]  R. Herrera,et al.  Rapid induction of c-fos transcription reveals quantitative linkage of RNA polymerase II and DNA topoisomerase I enzyme activities , 1990, Cell.

[35]  Nicholas R. Cozzarelli,et al.  DNA topology and its biological effects , 1990 .

[36]  S. Fields,et al.  A novel genetic system to detect protein–protein interactions , 1989, Nature.

[37]  L. Liu,et al.  DNA topoisomerase poisons as antitumor drugs. , 1989, Annual review of biochemistry.

[38]  E. Egyházi,et al.  Effects of anti-C23 (nucleolin) antibody on transcription of ribosomal DNA in Chironomus salivary gland cells. , 1988, Experimental cell research.

[39]  Leroy F. Liu,et al.  Transcription generates positively and negatively supercoiled domains in the template , 1988, Cell.

[40]  M. Kozak.  An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs. , 1987, Nucleic acids research.

[41]  R. Sternglanz,et al.  Need for DNA topoisomerase activity as a swivel for DNA replication for transcription of ribosomal RNA , 1987, Nature.

[42]  L. Breeden,et al.  Regulation of the yeast HO gene. , 1985, Cold Spring Harbor symposia on quantitative biology.

[43]  F. Amalric,et al.  Interrelations between the maturation of a 100 kDa nucleolar protein and pre rRNA synthesis in CHO cells. , 1984, Nucleic acids research.

[44]  V. Georgiev.  Virology , 1955, Nature.