The Carboxyl-Terminus of Human Immunodeficiency Virus Type 2 Circulating Recombinant form 01_AB Capsid Protein Affects Sensitivity to Human TRIM5α

Human immunodeficiency virus (HIV) type 2 shows limited geographical distribution compared with HIV type 1. Although 8 genetic groups of HIV type 2 (HIV-2) have been described, recombinant viruses between these groups are rarely observed. Recently, three HIV-2 patients in Japan were described with rapidly progressive, acquired immunodeficiency. These patients were infected with an A/B inter-group recombinant designated CRF01_AB. Here, we characterize the capsid protein (CA) encoded by the viruses from these patients. HIV-2 CRF01_AB CA showed unique amino acid sequence almost equally distinct from group A and group B viruses. Notably, HIV-2 CRF01_AB CA showed potent resistance to human TRIM5α. In addition to the previously identified amino acid position 119 in the N-terminal domain of CA, we found that HIV-2 CRF01_AB-specific amino acid substitutions in the C-terminal domain also were necessary for resistance to human TRIM5α. These results indicate that retroviruses can evade TRIM5α by substitution at residues within the C-terminal domain of CA.

[1]  A. Mitra,et al.  A structural model for the generation of continuous curvature on the surface of a retroviral capsid. , 2012, Journal of molecular biology.

[2]  T. Miyamoto,et al.  A Single Amino Acid of Human Immunodeficiency Virus Type 2 Capsid Protein Affects Conformation of Two External Loops and Viral Sensitivity to TRIM5α , 2011, PloS one.

[3]  J. Luban,et al.  Vpx rescues HIV-1 transduction of dendritic cells from the antiviral state established by type 1 interferon , 2011, Retrovirology.

[4]  Jeremy Luban,et al.  TRIM5 is an innate immune sensor for the retrovirus capsid lattice , 2011, Nature.

[5]  A. Ryo,et al.  A structural constraint for functional interaction between N-terminal and C-terminal domains in simian immunodeficiency virus capsid proteins , 2010, Retrovirology.

[6]  T. Shioda,et al.  Multiple sites in the N-terminal half of simian immunodeficiency virus capsid protein contribute to evasion from rhesus monkey TRIM5α-mediated restriction , 2010, Retrovirology.

[7]  W. Sugiura,et al.  HIV-2 CRF01_AB: First Circulating Recombinant Form of HIV-2 , 2010, Journal of acquired immune deficiency syndromes.

[8]  T. Shioda,et al.  Anti‐retroviral activity of TRIM5α , 2010, Reviews in medical virology.

[9]  S. Rowland-Jones,et al.  HIV-2 capsids distinguish high and low virus load patients in a West African community cohort. , 2009, Vaccine.

[10]  L. James,et al.  Active site remodeling switches HIV specificity of antiretroviral TRIMCyp , 2009, Nature Structural &Molecular Biology.

[11]  Mark Yeager,et al.  X-Ray Structures of the Hexameric Building Block of the HIV Capsid , 2009, Cell.

[12]  Toshihiro Sato,et al.  Phosphorylation of APOBEC3G by protein kinase A regulates its interaction with HIV-1 Vif , 2008, Nature Structural &Molecular Biology.

[13]  T. Shioda,et al.  Comparison of anti-viral activity of rhesus monkey and cynomolgus monkey TRIM5alphas against human immunodeficiency virus type 2 infection. , 2008, Virology.

[14]  Mark Yeager,et al.  The structural biology of HIV assembly. , 2008, Current opinion in structural biology.

[15]  David P Lane,et al.  Molecular simulations of protein dynamics: new windows on mechanisms in biology , 2008, EMBO reports.

[16]  J. Levy,et al.  A Single Amino Acid of the Human Immunodeficiency Virus Type 2 Capsid Affects Its Replication in the Presence of Cynomolgus Monkey and Human TRIM5αs , 2007, Journal of Virology.

[17]  S. Rowland-Jones,et al.  Out of Africa: what can we learn from HIV-2 about protective immunity to HIV-1? , 2007, Nature Immunology.

[18]  J. Levy,et al.  A single amino acid of the human immunodeficiency virus type 2 capsid affects its replication in the presence of cynomolgus monkey and human TRIM5alphas. , 2007, Journal of virology.

[19]  V. Hornak,et al.  Comparison of multiple Amber force fields and development of improved protein backbone parameters , 2006, Proteins.

[20]  A. Engelman,et al.  Proteasome Inhibition Reveals that a Functional Preintegration Complex Intermediate Can Be Generated during Restriction by Diverse TRIM5 Proteins , 2006, Journal of Virology.

[21]  T. Hope,et al.  Proteasome inhibitors uncouple rhesus TRIM5alpha restriction of HIV-1 reverse transcription and infection. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[22]  Joseph Sodroski,et al.  Specific recognition and accelerated uncoating of retroviral capsids by the TRIM5alpha restriction factor. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[23]  J. Luban,et al.  Cyclophilin A and TRIM5α Independently Regulate Human Immunodeficiency Virus Type 1 Infectivity in Human Cells , 2006, Journal of Virology.

[24]  Y. Nagai,et al.  A Specific Region of 37 Amino Acid Residues in the SPRY (B30.2) Domain of African Green Monkey TRIM5α Determines Species-Specific Restriction of Simian Immunodeficiency Virus SIVmac Infection , 2005, Journal of Virology.

[25]  J. Luban,et al.  TRIM5α selectively binds a restriction-sensitive retroviral capsid , 2005, Retrovirology.

[26]  K. Ikuta,et al.  Amino Acid 36 in the Human Immunodeficiency Virus Type 1 gp41 Ectodomain Controls Fusogenic Activity: Implications for the Molecular Mechanism of Viral Escape from a Fusion Inhibitor , 2005, Journal of Virology.

[27]  J. Sodroski,et al.  TRIM5alpha mediates the postentry block to N-tropic murine leukemia viruses in human cells. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[28]  G. Towers,et al.  The human and African green monkey TRIM5alpha genes encode Ref1 and Lv1 retroviral restriction factor activities. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[29]  A. Yang,et al.  Retrovirus resistance factors Ref1 and Lv1 are species-specific variants of TRIM5alpha. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[30]  F. Brun-Vézinet,et al.  Identification of a highly divergent HIV type 2 and proposal for a change in HIV type 2 classification. , 2004, AIDS research and human retroviruses.

[31]  C. M. Owens,et al.  The cytoplasmic body component TRIM5α restricts HIV-1 infection in Old World monkeys , 2004, Nature.

[32]  J. Ponder,et al.  Force fields for protein simulations. , 2003, Advances in protein chemistry.

[33]  M. Malim,et al.  Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein , 2002, Nature.

[34]  G. Gottlieb,et al.  Equal plasma viral loads predict a similar rate of CD4+ T cell decline in human immunodeficiency virus (HIV) type 1- and HIV-2-infected individuals from Senegal, West Africa. , 2002, The Journal of infectious diseases.

[35]  A. Sali,et al.  Protein Structure Prediction and Structural Genomics , 2001, Science.

[36]  Alessandro Guffanti,et al.  The tripartite motif family identifies cell compartments , 2001, The EMBO journal.

[37]  C. K. Chu,et al.  Molecular Modeling Approach to Understanding the Mode of Action of l-Nucleosides as Antiviral Agents , 2001, Antimicrobial Agents and Chemotherapy.

[38]  F. Gao,et al.  Origin of HIV-1 in the chimpanzee Pan troglodytes troglodytes , 1999, Nature.

[39]  W. Sundquist,et al.  Structure of the carboxyl-terminal dimerization domain of the HIV-1 capsid protein. , 1997, Science.

[40]  I. Thior,et al.  Reduced rate of disease development after HIV-2 infection as compared to HIV-1. , 1994, Science.

[41]  J. Bull,et al.  An Empirical Test of Bootstrapping as a Method for Assessing Confidence in Phylogenetic Analysis , 1993 .

[42]  G. Nolan,et al.  Production of high-titer helper-free retroviruses by transient transfection. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[43]  F. Gao,et al.  Human infection by genetically diverse SIVSM-related HIV-2 in West Africa , 1992, Nature.

[44]  Andreas Meyerhans,et al.  Genetic organization of a chimpanzee lentivirus related to HIV-1 , 1990, Nature.

[45]  G. Ciccotti,et al.  Numerical Integration of the Cartesian Equations of Motion of a System with Constraints: Molecular Dynamics of n-Alkanes , 1977 .

[46]  A. Dion,et al.  Experimental infection of a cat kidney cell line with the mouse mammary tumor virus. , 1976, Cancer research.