Effects of sludge pretreatments and organic acids on hydrogen production by anaerobic fermentation.

Using apple pomace (AP) as substrate, a series of batch experiments were conducted to investigate the effects of activated sludge pretreated by ultraviolet and ultrasonic on the anaerobic fermentative bio-H(2) production. The results show that a maximum cumulative H(2) production (CHP(m)) of 107.0mlg(-1)-TS and an average H(2) production rate (AHPR) of 15.0mlg(-1)-TSh(-1) were obtained by using the pretreated sludge that was irradiated by ultraviolet lamp (25w) for 15min as inoculant. Based on the optimal sludge pretreatment method, the experiments of bio-H(2) production under the optimal substrate pretreatment condition (soaked in the ammonia liquor of 6% for 24h) were further carried out, and the CHP(m) of 138.8mlg(-1)-TS was achieved, increased by 80.6% compared to the un-pretreated group (76.8mlg(-1)-TS). The effects of the various organic acids on the fermentative H(2) production were also investigated under the above optimal sludge pretreatment condition.

[1]  D. Lee,et al.  The effects of pH on carbon material and energy balances in hydrogen-producing Clostridium tyrobutyricum JM1. , 2008, Bioresource technology.

[2]  Lawrence Pitt,et al.  Biohydrogen production: prospects and limitations to practical application , 2004 .

[3]  Hui Wang,et al.  Biohydrogen production from cornstalk wastes by anaerobic fermentation with activated sludge , 2010 .

[4]  Debabrata Das,et al.  Hydrogen production by biological processes: a survey of literature , 2001 .

[5]  Baikun Li,et al.  Biohydrogen production from molasses by anaerobic fermentation with a pilot-scale bioreactor system , 2006 .

[6]  R. Dinsdale,et al.  Hydrogen production from sewage sludge using mixed microflora inoculum: effect of pH and enzymatic pretreatment. , 2008, Bioresource technology.

[7]  S. Summerfelt,et al.  Inactivation of bacteria using ultraviolet irradiation in a recirculating salmonid culture system , 2005 .

[8]  Chiu-Yue Lin,et al.  Biohydrogen production from sucrose using base-enriched anaerobic mixed microflora , 2006 .

[9]  Bo Xiao,et al.  Effect of low power ultrasonic radiation on anaerobic biodegradability of sewage sludge. , 2009, Bioresource technology.

[10]  Dae Sung Lee,et al.  Biological hydrogen production by immobilized cells of Clostridium tyrobutyricum JM1 isolated from a food waste treatment process. , 2008, Bioresource technology.

[11]  Jianlong Wang,et al.  Comparison of different pretreatment methods for enriching hydrogen-producing bacteria from digested sludge , 2008 .

[12]  Patrick C. Hallenbeck,et al.  Biological hydrogen production; fundamentals and limiting processes , 2002 .

[13]  Hui Wang,et al.  Effects of pH value and substrate concentration on hydrogen production from the anaerobic fermentation of glucose , 2008 .

[14]  Debabrata Das,et al.  ADVANCES IN BIOLOGICAL HYDROGEN PRODUCTION PROCESSES , 2008 .

[15]  G. Nakhla,et al.  The effect of heat pretreatment temperature on fermentative hydrogen production using mixed cultures , 2008 .

[16]  Baikun Li,et al.  Anaerobic biohydrogen production from monosaccharides by a mixed microbial community culture. , 2008, Bioresource technology.

[17]  P. N. Sarma,et al.  Effect of various pretreatment methods on anaerobic mixed microflora to enhance biohydrogen production utilizing dairy wastewater as substrate. , 2008, Bioresource technology.

[18]  Hui Wang,et al.  Biohydrogen production from apple pomace by anaerobic fermentation with river sludge , 2010 .