Biocompatible fluorescent silicon nanocrystals for single-molecule tracking and fluorescence imaging

Lack of photobleaching and blinking of new silicon nanocrystals allows direct observation of single-molecule receptor internalization and large-scale mosaicism in the plasma membrane.

[1]  S. Nishimura,et al.  Both MHC class II and its GPI-anchored form undergo hop diffusion as observed by single-molecule tracking. , 2008, Biophysical journal.

[2]  Z. Lenkei,et al.  Small and stable sulfobetaine zwitterionic quantum dots for functional live-cell imaging. , 2010, Journal of the American Chemical Society.

[3]  Akiyoshi Hoshino,et al.  Water-soluble photoluminescent silicon quantum dots. , 2005, Angewandte Chemie.

[4]  Ken-Tye Yong,et al.  Biocompatible luminescent silicon quantum dots for imaging of cancer cells. , 2008, ACS nano.

[5]  J. Valenta,et al.  Luminescence blinking of a Si quantum dot in a SiO2 shell , 2005 .

[6]  Colin Echeverría Aitken,et al.  An oxygen scavenging system for improvement of dye stability in single-molecule fluorescence experiments. , 2008, Biophysical journal.

[7]  Daniel S. Terry,et al.  Enhanced photostability of cyanine fluorophores across the visible spectrum , 2012, Nature Methods.

[8]  Akihiro Kusumi,et al.  Paradigm shift of the plasma membrane concept from the two-dimensional continuum fluid to the partitioned fluid: high-speed single-molecule tracking of membrane molecules. , 2005, Annual review of biophysics and biomolecular structure.

[9]  R. Rigler,et al.  Fluorescence correlation spectroscopy with high count rate and low background: analysis of translational diffusion , 1993, European Biophysics Journal.

[10]  Taekjip Ha,et al.  Photophysics of fluorescent probes for single-molecule biophysics and super-resolution imaging. , 2012, Annual review of physical chemistry.

[11]  Grace M. Credo,et al.  External quantum efficiency of single porous silicon nanoparticles , 1999 .

[12]  Tomonobu M. Watanabe,et al.  Reduction in nonfluorescence state of quantum dots on an immunofluorescence staining. , 2006, Biochemical and biophysical research communications.

[13]  Shuming Nie,et al.  Semiconductor nanocrystals: structure, properties, and band gap engineering. , 2010, Accounts of chemical research.

[14]  Daniel Choquet,et al.  Surface Mobility of Postsynaptic AMPARs Tunes Synaptic Transmission , 2008, Science.

[15]  R. Tsien,et al.  On/off blinking and switching behaviour of single molecules of green fluorescent protein , 1997, Nature.

[16]  A. Kusumi,et al.  Confined lateral diffusion of membrane receptors as studied by single particle tracking (nanovid microscopy). Effects of calcium-induced differentiation in cultured epithelial cells. , 1993, Biophysical journal.

[17]  M. Tamura,et al.  Microenvironment of endosomal aqueous phase investigated by the mobility of microparticles using fluorescence correlation spectroscopy. , 2001, Biochemical and biophysical research communications.

[18]  Y. Nodasaka,et al.  Preparation and characterization of thiacalix[4]arene coated water-soluble CdSe/ZnS quantum dots as a fluorescent probe for Cu2+ ions. , 2007, Combinatorial chemistry & high throughput screening.

[19]  G. Ya. Wiederschain,et al.  The Molecular Probes handbook. A guide to fluorescent probes and labeling technologies , 2011, Biochemistry (Moscow).

[20]  Dennis E. Koppel,et al.  Analysis of Macromolecular Polydispersity in Intensity Correlation Spectroscopy: The Method of Cumulants , 1972 .

[21]  C. Hopkins,et al.  Role of the human transferrin receptor cytoplasmic domain in endocytosis: localization of a specific signal sequence for internalization , 1990, The Journal of cell biology.

[22]  M. Nayfeh,et al.  Excitation of size selected nanocrystallites in porous silicon , 1998 .

[23]  Daniel T Chiu,et al.  Deconvolving single-molecule intensity distributions for quantitative microscopy measurements. , 2007, Biophysical journal.

[24]  J. Valenta,et al.  Narrow luminescence linewidth of a silicon quantum dot. , 2005, Physical review letters.

[25]  D. F. Eaton.  International Union of Pure and Applied Chemistry Organic Chemistry Division Commission on Photochemistry. Reference materials for fluorescence measurement. , 1988, Journal of photochemistry and photobiology. B, Biology.

[26]  N. Andrews,et al.  Comparison of the Interactions of Transferrin Receptor and Transferrin Receptor 2 with Transferrin and the Hereditary Hemochromatosis Protein HFE* , 2000, The Journal of Biological Chemistry.

[27]  D. Choquet,et al.  Multiple Routes for Glutamate Receptor Trafficking: Surface Diffusion and Membrane Traffic Cooperate to Bring Receptors to Synapses , 2006, Science's STKE.

[28]  M. Howarth,et al.  Quantum dot targeting with lipoic acid ligase and HaloTag for single-molecule imaging on living cells. , 2012, ACS nano.

[29]  Hong Ding,et al.  In vivo targeted cancer imaging, sentinel lymph node mapping and multi-channel imaging with biocompatible silicon nanocrystals. , 2011, ACS nano.

[30]  Wilfried van Sark,et al.  Blueing, bleaching, and blinking of single CdSe/ZnS quantum dots , 2002 .

[31]  K. A. Smith,et al.  Permeability studies with cellulosic membranes. , 1971, Journal of biomedical materials research.

[32]  Hong Ding,et al.  Bioconjugation of luminescent silicon quantum dots for selective uptake by cancer cells. , 2011, Bioconjugate chemistry.

[33]  Maxime Dahan,et al.  Multiple association states between glycine receptors and gephyrin identified by SPT analysis. , 2007, Biophysical journal.

[34]  W. Melhuish,et al.  QUANTUM EFFICIENCIES OF FLUORESCENCE OF ORGANIC SUBSTANCES: EFFECT OF SOLVENT AND CONCENTRATION OF THE FLUORESCENT SOLUTE1 , 1961 .

[35]  Michael D. Cahalan,et al.  A Decade of Imaging Cellular Motility and Interaction Dynamics in the Immune System , 2012, Science.

[36]  A Kusumi,et al.  Single molecule imaging of green fluorescent proteins in living cells: E-cadherin forms oligomers on the free cell surface. , 2001, Biophysical journal.

[37]  P. Debenedetti,et al.  Stability of proteins in the presence of carbohydrates; experiments and modeling using scaled particle theory. , 2007, Biophysical chemistry.

[38]  Akihiro Kusumi,et al.  Single-molecule imaging analysis of Ras activation in living cells. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[39]  L Wang,et al.  Silicon nanoparticles as a luminescent label to DNA. , 2004, Bioconjugate chemistry.

[40]  S. Mayor,et al.  Cholesterol‐Sensitive Cdc42 Activation Regulates Actin Polymerization for Endocytosis via the GEEC Pathway , 2007, Traffic.

[41]  Christopher L. Brown,et al.  Rapid purification of EGFP, EYFP, and ECFP with high yield and purity. , 2005, Protein expression and purification.

[42]  Eli Ruckenstein,et al.  Water-Soluble Poly(acrylic acid) Grafted Luminescent Silicon Nanoparticles and Their Use as Fluorescent Biological Staining Labels , 2004 .

[43]  Akihiro Kusumi,et al.  Phospholipids undergo hop diffusion in compartmentalized cell membrane , 2002, The Journal of cell biology.

[44]  Eric R. Prossnitz,et al.  Full characterization of GPCR monomer–dimer dynamic equilibrium by single molecule imaging , 2011, The Journal of cell biology.

[45]  L. Canham.  Silicon quantum wire array fabrication by electrochemical and chemical dissolution of wafers , 1990 .

[46]  N. Wang,et al.  Conjugation of the photoluminescent silicon nanoparticles to streptavidin. , 2008, Bioconjugate chemistry.

[47]  John Silcox,et al.  Non-blinking semiconductor nanocrystals , 2009, Nature.

[48]  Jonathan A Javitch,et al.  Cyanine fluorophore derivatives with enhanced photostability , 2011, Nature Methods.

[49]  D. Toomre,et al.  A new wave of cellular imaging. , 2010, Annual review of cell and developmental biology.

[50]  C. J. Lewis,et al.  Cyanine dye labeling reagents: sulfoindocyanine succinimidyl esters. , 1993, Bioconjugate chemistry.

[51]  M. Swihart,et al.  Surface functionalization of silicon nanoparticles produced by laser-driven pyrolysis of silane followed by HF-HNO3 etching. , 2004, Langmuir : the ACS journal of surfaces and colloids.

[52]  Mike Heilemann,et al.  A reducing and oxidizing system minimizes photobleaching and blinking of fluorescent dyes. , 2008, Angewandte Chemie.

[53]  Watt W Webb,et al.  Blinking and nonradiant dark fraction of water-soluble quantum dots in aqueous solution. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[54]  K. Jacobson,et al.  Detection of temporary lateral confinement of membrane proteins using single-particle tracking analysis. , 1995, Biophysical journal.

[55]  Daniel Choquet,et al.  The Interaction between Stargazin and PSD-95 Regulates AMPA Receptor Surface Trafficking , 2007, Neuron.

[56]  D. J. Lockwood,et al.  Stabilization of porous silicon electroluminescence by surface passivation with controlled covalent bonds , 2003 .

[57]  T. Gregorkiewicz,et al.  Red spectral shift and enhanced quantum efficiency in phonon-free photoluminescence from silicon nanocrystals. , 2010, Nature nanotechnology.

[58]  Markus Sauer,et al.  Spectroscopic study and evaluation of red-absorbing fluorescent dyes. , 2003, Bioconjugate chemistry.

[59]  Derek Toomre,et al.  Spatial control of EGF receptor activation by reversible dimerization on living cells , 2010, Nature.

[60]  B. Dubertret,et al.  Towards non-blinking colloidal quantum dots. , 2008, Nature materials.

[61]  Akihiro Kusumi,et al.  Fluorescence imaging for monitoring the colocalization of two single molecules in living cells. , 2005, Biophysical journal.

[62]  R. Tsien,et al.  Partitioning of Lipid-Modified Monomeric GFPs into Membrane Microdomains of Live Cells , 2002, Science.

[63]  J F Murray,et al.  Determination of sulfhydryl groups with 2,2'- or 4,4'-dithiodipyridine. , 1967, Archives of biochemistry and biophysics.

[64]  B. Baird,et al.  Nanobiotechnology and cell biology: micro- and nanofabricated surfaces to investigate receptor-mediated signaling. , 2008, Annual review of biophysics.

[65]  Louis E. Brus,et al.  Electronic Structure and Luminescence of 1.1- and 1.4-nm Silicon Nanocrystals: Oxide Shell versus Hydrogen Passivation , 2003 .

[66]  Enrico Gratton,et al.  Detection of luminescent single ultrasmall silicon nanoparticles using fluctuation correlation spectroscopy , 2000 .

[67]  Yuling Yan,et al.  Optical switch probes and optical lock-in detection (OLID) imaging microscopy: high-contrast fluorescence imaging within living systems. , 2011, The Biochemical journal.

[68]  J. Vela,et al.  "Giant" multishell CdSe nanocrystal quantum dots with suppressed blinking. , 2008, Journal of the American Chemical Society.

[69]  K Dane Wittrup,et al.  Monovalent, reduced-size quantum dots for imaging receptors on living cells , 2008, Nature Methods.

[70]  John C. Thomas,et al.  The determination of log normal particle size distributions by dynamic light scattering , 1987 .

[71]  L. Mitas,et al.  Observation of a magic discrete family of ultrabright Si nanoparticles , 2002 .