Isoenzyme and Protein Patterns of In Vitro Micropropagated Plantlets of Gentiana Lutea L. after Application of Various Growth Regulators

ABSTRACT The electrophoretic patterns of reduced proteins and ADH, ACPH, EST and GOT from leaves of in vitro micropropagated plantlets of Gentiana lutea L. were investigated. Five variants of Murashige and Skoog (MS) media differing in the type and concentration of growth regulators were used. Qualitative isoenzyme variations between the in vitro plantlets were found for ADH, ACPH and EST, whereas the variations for GOT isoenzymes were only quantitative. The isoenzyme variation was discussed in respect to the differences in the type of growth regulators included in the nutrient media.

[1]  S. Raychaudhuri,et al.  Isozyme and RAPD markers in relation to in vitro morphogenesis and in vivo development ofVigna radiata (L.) Wilczek , 2003, The Botanical Review.

[2]  H. S. Chawla.  Regeneration responses of callus from different expiants and changes in isozymes during morphogenesis in wheat , 1989, Biologia Plantarum.

[3]  P. Das,et al.  Changes in Isozyme Patterns During in Vitro Regeneration from Cotyledon Explants of Brassica Species , 1999, Biologia Plantarum.

[4]  D. Astley,et al.  Effect of regeneration procedures on genetic diversity in Brassica napus and B. rapa as estimated by isozyme analysis , 1997, Genetic Resources and Crop Evolution.

[5]  I. Momčilović,et al.  Micropropagation of four Gentiana species (G. lutea, G. cruciata, G. purpurea and G. acaulis) , 1997, Plant Cell, Tissue and Organ Culture.

[6]  C. Shaw,et al.  Starch gel electrophoresis of enzymes—A compilation of recipes , 1970, Biochemical Genetics.

[7]  K. Majourhat,et al.  Morphological variations and isoenzyme polymorphism of date palm clones from in vitro culture acclimatized and established on soil in South Morocco , 2004, Euphytica.

[8]  N. Menković,et al.  Quantitative determination of secoiridoid and gamma-pyrone compounds in Gentiana lutea cultured in vitro. , 2009, Planta medica.

[9]  O. Gamborg,et al.  Plant Cell, Tissue and Organ Culture , 1995, Springer Lab Manual.

[10]  S. L. Bunting,et al.  Serum Protein Electrophoresis in Acrylamide Gel: Patterns from Normal Human Subjects , 1965, Science.

[11]  F. Skoog,et al.  A revised medium for rapid growth and bio assays with tobacco tissue cultures , 1962 .