Evaluation of Surface Microtopography Engineered by Direct Laser Interference for Bacterial Anti-Biofouling.

Modification of the biomaterial surface topography is a promising strategy to prevent bacterial adhesion and biofilm formation. In this study, we use direct laser interference patterning (DLIP) to modify polystyrene surface topography at sub-micrometer scale. The results revealed that three-dimensional micrometer structures have a profound impact on bacterial adhesion. Thus, line- and pillar-like patterns enhanced S. aureus adhesion, whereas complex lamella microtopography reduced S. aureus adhesion in static and continuous flow culture conditions. Interestingly, lamella-like textured surfaces retained the capacity to inhibit S. aureus adhesion both when the surface is coated with human serum proteins and when the material is implanted subcutaneously in a foreign-body associated infection model.

[1]  Alexander M. Tatara,et al.  Evolving strategies for preventing biofilm on implantable materials , 2013 .

[2]  J. Costerton,et al.  Bacterial biofilms: a common cause of persistent infections. , 1999, Science.

[3]  Robert D. Boyd,et al.  Use of the atomic force microscope to determine the effect of substratum surface topography on bacterial adhesion , 2002 .

[4]  Y. Missirlis,et al.  Concise review of mechanisms of bacterial adhesion to biomaterials and of techniques used in estimating bacteria-material interactions. , 2004, European cells & materials.

[5]  Andrés Fabián Lasagni,et al.  Control of cell growth direction by direct fabrication of periodic micro- and submicrometer arrays on polymers , 2012 .

[6]  C. Solano,et al.  Bap, a Staphylococcus aureus Surface Protein Involved in Biofilm Formation , 2001, Journal of bacteriology.

[7]  F. Götz.  Staphylococci in colonization and disease: prospective targets for drugs and vaccines. , 2004, Current opinion in microbiology.

[8]  Elena P Ivanova,et al.  Antibacterial surfaces: the quest for a new generation of biomaterials. , 2013, Trends in biotechnology.

[9]  Ilker S. Bayer,et al.  Advances in top-down and bottom-up surface nanofabrication: techniques, applications & future prospects. , 2012, Advances in colloid and interface science.

[10]  Lauren D. Zarzar,et al.  Structural transformation by electrodeposition on patterned substrates (STEPS): a new versatile nanofabrication method. , 2012, Nano letters.

[11]  L. Jespersen,et al.  Attachment behaviour of Escherichia coli K12 and Salmonella Typhimurium P6 on food contact surfaces for food transportation. , 2012, Food microbiology.

[12]  D. Nicolau,et al.  Micro-structures modulate bacterial cell viability and attachment , 2009 .

[13]  Christopher J. Long,et al.  Engineered nanoforce gradients for inhibition of settlement (attachment) of swimming algal spores. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[14]  James F. Schumacher,et al.  Impact of engineered surface microtopography on biofilm formation of Staphylococcus aureus , 2007, Biointerphases.

[15]  Timothy J. Foster,et al.  Adhesion, invasion and evasion: the many functions of the surface proteins of Staphylococcus aureus , 2013, Nature Reviews Microbiology.

[16]  C. Solano,et al.  Relevant Role of Fibronectin-Binding Proteins in Staphylococcus aureus Biofilm-Associated Foreign-Body Infections , 2009, Infection and Immunity.

[17]  Thomas R. Kiehl,et al.  Development of antifouling surfaces to reduce bacterial attachment , 2013 .

[18]  Adam W Feinberg,et al.  Engineered antifouling microtopographies – effect of feature size, geometry, and roughness on settlement of zoospores of the green alga Ulva , 2007, Biofouling.

[19]  Ronn S. Friedlander,et al.  Bacterial flagella explore microscale hummocks and hollows to increase adhesion , 2013, Proceedings of the National Academy of Sciences.

[20]  D. Nicolau,et al.  Arrays of nano-structured surfaces to probe the adhesion and viability of bacteria , 2012 .

[21]  D. Campoccia,et al.  Study of Staphylococcus Aureus Adhesion on a Novel Nanostructured Surface by Chemiluminometry , 2006, The International journal of artificial organs.

[22]  J. Colligon,et al.  Use of the atomic force microscope to determine the effect of substratum surface topography on the ease of bacterial removal. , 2006, Colloids and surfaces. B, Biointerfaces.

[23]  Francois Malherbe,et al.  Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus Attachment Patterns on Glass Surfaces with Nanoscale Roughness , 2009, Current Microbiology.

[24]  I. Lasa,et al.  SarA and not σB is essential for biofilm development by Staphylococcus aureus , 2003, Molecular microbiology.

[25]  I. Banerjee,et al.  Sustained release of antibiotic from polyurethane coated implant materials , 2009, Journal of materials science. Materials in medicine.

[26]  C. Siedlecki,et al.  Submicron-textured biomaterial surface reduces staphylococcal bacterial adhesion and biofilm formation. , 2012, Acta biomaterialia.

[27]  Leo H. Koole,et al.  New Strategies in the Development of Antimicrobial Coatings: The Example of Increasing Usage of Silver and Silver Nanoparticles , 2011 .

[28]  K. Whitehead,et al.  The effect of surface topography on the retention of microorganisms , 2006 .

[29]  Carmen I. Moraru,et al.  Effect of Micro- and Nanoscale Topography on the Adhesion of Bacterial Cells to Solid Surfaces , 2013, Applied and Environmental Microbiology.

[30]  Blaise R. Boles,et al.  Functional Amyloids Composed of Phenol Soluble Modulins Stabilize Staphylococcus aureus Biofilms , 2012, PLoS pathogens.

[31]  Joanna Verran,et al.  Retention of microbial cells in substratum surface features of micrometer and sub-micrometer dimensions. , 2005, Colloids and surfaces. B, Biointerfaces.

[32]  P. Stoodley,et al.  Developmental regulation of microbial biofilms. , 2002, Current opinion in biotechnology.

[33]  J. Ghigo.  Natural conjugative plasmids induce bacterial biofilm development , 2001, Nature.

[34]  Nathaniel C. Cady,et al.  Nano and Microscale Topographies for the Prevention of Bacterial Surface Fouling , 2014 .

[35]  S. Shiratori,et al.  Porous surface structure fabricated by breath figures that suppresses Pseudomonas aeruginosa biofilm formation. , 2013, ACS applied materials & interfaces.

[36]  V. Nizet,et al.  Role of Staphylococcus aureus Catalase in Niche Competition against Streptococcus pneumoniae , 2008, Journal of bacteriology.

[37]  G. Donelli,et al.  Polyurethane anionomers containing metal ions with antimicrobial properties: thermal, mechanical and biological characterization. , 2010, Acta biomaterialia.

[38]  James M. Anderson,et al.  S. epidermidis biofilm formation: effects of biomaterial surface chemistry and serum proteins. , 2007, Journal of biomedical materials research. Part A.

[39]  Paul Stoodley,et al.  Biofilm formation and dispersal and the transmission of human pathogens. , 2005, Trends in microbiology.

[40]  R. Kolter,et al.  Biofilm formation as microbial development. , 2000, Annual review of microbiology.